Restriction map for pF1KB6764
 (Circular) (Six-base) MAPSORT of: pF1KB6764.seq  Check: 3101  from: 1  to: 3557



pF1KB6764  3557 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AccI GT'mk_AC

Cuts at:    595     595 
   Size:       3557
 
Acc65I G'GTAC_C

Cuts at:    573     573 
   Size:       3557
 
AclI AA'CG_TT

Cuts at:   3397    3397 
   Size:       3557
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1382    1814    2561    2829    1382 
   Size:        432     747     268    2110

  Fragments arranged by size: 

                2110     747     432     268
 
AflIII A'CryG_T

Cuts at:    909    2013    2990     909 
   Size:       1104     977    1476

  Fragments arranged by size: 

                1476    1104     977
 
AgeI A'CCGG_T

Cuts at:   1945    1945 
   Size:       3557
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    427     427 
   Size:       3557
 
AlwNI CAG_nnn'CTG

Cuts at:    862    2429     862 
   Size:       1567    1990
 
ApaLI G'TGCA_C

Cuts at:   2327    2327 
   Size:       3557
 
ApoI r'AATT_y

Cuts at:    561    2910    3166     561 
   Size:       2349     256     952

  Fragments arranged by size: 

                2349     952     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3557
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3557
 
AvaI C'yCGr_G

Cuts at:    577     577 
   Size:       3557
 
BamHI G'GATC_C

Cuts at:    582     582 
   Size:       3557
 
BanI G'GyrC_C

Cuts at:    573     987    1235    1270     573 
   Size:        414     248      35    2860

  Fragments arranged by size: 

                2860     414     248      35
 
BanII G_rGCy'C

Cuts at:    571    1601     571 
   Size:       1030    2527
 
BbeI G_GCGC'C

Cuts at:    991    1239     991 
   Size:        248    3309
 
BciVI GTATCCnnnnn_n'

Cuts at:   1455    2216    3557    1455 
   Size:        761    1341    1455

  Fragments arranged by size: 

                1455    1341     761
 
BclI T'GATC_A

Cuts at:   1077    1077 
   Size:       3557
 
BglI GCCn_nnn'nGGC

Cuts at:    112     112 
   Size:       3557
 
BglII A'GATC_T

Cuts at:   1072    3109    1072 
   Size:       2037    1520
 
BlpI GC'TnA_GC

Cuts at:    669     669 
   Size:       3557
 
Bme1580I G_kGCm'C

Cuts at:   1182    1275    2331    1182 
   Size:         93    1056    2408

  Fragments arranged by size: 

                2408    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    769     850    1185    3329     769 
   Size:         81     335    2144     997

  Fragments arranged by size: 

                2144     997     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1924    1924 
   Size:       3557
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    323     323 
   Size:       3557
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    733    1612    2104    2402     733 
   Size:        879     492     298    1888

  Fragments arranged by size: 

                1888     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3261    3261 
   Size:       3557
 
BsaAI yAC'GTr

Cuts at:    910     910 
   Size:       3557
 
BsaBI GATnn'nnATC

Cuts at:      6    1071    3108       6 
   Size:       1065    2037     455

  Fragments arranged by size: 

                2037    1065     455
 
BsaHI Gr'CG_yC

Cuts at:    988    1236    1938    3434     988 
   Size:        248     702    1496    1111

  Fragments arranged by size: 

                1496    1111     702     248
 
BsaWI w'CCGG_w

Cuts at:    746    1267    1945    2219    2366    2777     746 
   Size:        521     678     274     147     411    1526

  Fragments arranged by size: 

                1526     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    489     519     489 
   Size:         30    3527
 
BseYI C'CCAG_C

Cuts at:    982    1740    2317    2813     982 
   Size:        758     577     496    1726

  Fragments arranged by size: 

                1726     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1099    1145    2353     104 
   Size:        995      46    1208    1308

  Fragments arranged by size: 

                1308    1208     995      46
 
BsiHKAI G_wGCw'C

Cuts at:    571    1349    1539    2331     571 
   Size:        778     190     792    1797

  Fragments arranged by size: 

                1797     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    571    1182    1275    1349    1539    1601    2331     571 
   Size:        611      93      74     190      62     730    1797

  Fragments arranged by size: 

                1797     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2777    2777 
   Size:       3557
 
BspHI T'CATG_A

Cuts at:   2733    2733 
   Size:       3557
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    607     821    1123    1504     607 
   Size:        214     302     381    2660

  Fragments arranged by size: 

                2660     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1849    1903    3387    3552      50 
   Size:       1799      54    1484     165      55

  Fragments arranged by size: 

                1799    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1469    1469 
   Size:       3557
 
BsrFI r'CCGG_y

Cuts at:   1555    1736    1945    1555 
   Size:        181     209    3167

  Fragments arranged by size: 

                3167     209     181
 
BssSI C'ACGA_G

Cuts at:    281    1828    2186     281 
   Size:       1547     358    1652

  Fragments arranged by size: 

                1652    1547     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    385     934     385 
   Size:        549    3008
 
BstBI TT'CG_AA

Cuts at:   1918    1918 
   Size:       3557
 
BstYI r'GATC_y

Cuts at:      1     582    1049    1072    1407    1653    2665    2751    2763 
   Size:        581     467      23     335     246    1012      86      12

Cuts at:   2763    3109       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     581     467     449     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    411    1507    1688    1851     411 
   Size:       1096     181     163    2117

  Fragments arranged by size: 

                2117    1096     181     163
 
BtsI GCAGTG_nn'

Cuts at:    411     800     411 
   Size:        389    3168
 
DraI TTT'AAA

Cuts at:    556     556 
   Size:       3557
 
DrdI GACnn_nn'nnGTC

Cuts at:   1263    2121    1263 
   Size:        858    2699
 
EaeI y'GGCC_r

Cuts at:    185     254    1142    1316    1707    1734    3079     185 
   Size:         69     888     174     391      27    1345     663

  Fragments arranged by size: 

                1345     888     663     391     174      69      27
 
EagI C'GGCC_G

Cuts at:   1142    1142 
   Size:       3557
 
EarI CTCTTCn'nnn_

Cuts at:   1580    1790    1580 
   Size:        210    3347
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1765    2075    2221    3367    1765 
   Size:        310     146    1146    1955

  Fragments arranged by size: 

                1955    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    569     569 
   Size:       3557
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    323    1382    1814    2561    2829     323 
   Size:       1059     432     747     268    1051

  Fragments arranged by size: 

                1059    1051     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    696     696 
   Size:       3557
 
EcoRI G'AATT_C

Cuts at:    561     561 
   Size:       3557
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1576    1576 
   Size:       3557
 
FspI TGC'GCA

Cuts at:   1338    1338 
   Size:       3557
 
HaeII r_GCGC'y

Cuts at:    991    1239    2261    3240     991 
   Size:        248    1022     979    1308

  Fragments arranged by size: 

                1308    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1399    1429    1431    1461    1399 
   Size:         30       2      30    3495

  Fragments arranged by size: 

                3495      30      30       2
 
HincII GTy'rAC

Cuts at:    596     596 
   Size:       3557
 
KasI G'GCGC_C

Cuts at:    987    1235     987 
   Size:        248    3309
 
KpnI G_GTAC'C

Cuts at:    577     577 
   Size:       3557
 
MluI A'CGCG_T

Cuts at:   2990    2990 
   Size:       3557
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2228    2412    2228 
   Size:        184    3373
 
MscI TGG'CCA

Cuts at:    187     256    1318     187 
   Size:         69    1062    2426

  Fragments arranged by size: 

                2426    1062      69
 
MslI CAynn'nnrTG

Cuts at:   1546    1673    1546 
   Size:        127    3430
 
MspA1I CmG'CkG

Cuts at:    668     982    1342    2355    2600     668 
   Size:        314     360    1013     245    1625

  Fragments arranged by size: 

                1625    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1738    1738 
   Size:       3557
 
NarI GG'CG_CC

Cuts at:    988    1236     988 
   Size:        248    3309
 
NgoMIV G'CCGG_C

Cuts at:   1736    1736 
   Size:       3557
 
NspI r_CATG'y

Cuts at:    610    1110    2017     610 
   Size:        500     907    2150

  Fragments arranged by size: 

                2150     907     500
 
PciI A'CATG_T

Cuts at:   2013    2013 
   Size:       3557
 
PmeI GTTT'AAAC

Cuts at:    556     556 
   Size:       3557
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2696    2728    2696 
   Size:         32    3525
 
PstI C_TGCA'G

Cuts at:    406     604    1289     406 
   Size:        198     685    2674

  Fragments arranged by size: 

                2674     685     198
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3557
 
PvuII CAG'CTG

Cuts at:    982    1342     982 
   Size:        360    3197
 
RsrII CG'GwC_CG

Cuts at:   1752    1752 
   Size:       3557
 
SacI G_AGCT'C

Cuts at:    571     571 
   Size:       3557
 
SalI G'TCGA_C

Cuts at:    594     594 
   Size:       3557
 
SapI GCTCTTCn'nnn_

Cuts at:   1580    1790    1580 
   Size:        210    3347
 
SbfI CC_TGCA'GG

Cuts at:    604     604 
   Size:       3557
 
ScaI AGT'ACT

Cuts at:   3074    3074 
   Size:       3557
 
SfcI C'TryA_G

Cuts at:     33     219     402     437     600    1285    2278    2469      33 
   Size:        186     183      35     163     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     191     186     183     163      35
            
 
SfoI GGC'GCC

Cuts at:    989    1237     989 
   Size:        248    3309
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3557
 
SmaI CCC'GGG

Cuts at:    579     579 
   Size:       3557
 
SmlI C'TyrA_G

Cuts at:    712    1627    2119    2381     712 
   Size:        915     492     262    1888

  Fragments arranged by size: 

                1888     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3557
 
SphI G_CATG'C

Cuts at:    610    1110     610 
   Size:        500    3057
 
StyI C'CwwG_G

Cuts at:    691    1045     691 
   Size:        354    3203
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    477    1140    1273    1732    1941     477 
   Size:        663     133     459     209    2093

  Fragments arranged by size: 

                2093     663     459     209     133
 
TatI w'GTAC_w

Cuts at:   3072    3072 
   Size:       3557
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3074    3106    3074 
   Size:         32    3525
 
Tth111I GACn'n_nGTC

Cuts at:   1354    1354 
   Size:       3557
 
XbaI T'CTAG_A

Cuts at:    588     588 
   Size:       3557
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1052    1052 
   Size:       3557
 
XmaI C'CCGG_G

Cuts at:    577     577 
   Size:       3557
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI   NgoMIV     NspI     PciI     PmeI     PpiI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI    BmgBI
     BmtI   Bpu10I    BseRI     BsgI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI
   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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