Restriction map for pF1KE0208
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AfaI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvrII       
BamHI       BbeI        BbrPI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiWI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrDI       
BsrGI       BssSI       Bst1107I    BstAPI      BstEII      
BstPI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      Eam1105I    Eco47III    
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MroI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NlaIII      NotI        NruI        
NsiI        NspI        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfoI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        SwaI        Tth111I     XbaI        
XmaCI       XmaI        


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# ONE CUT ENZYMES (   60 enzymes) #
###################################

AccI          910  GTCTAC               GT^[AC][GT]AC
AflIII        233  ACGCGT               A^C[GA][CT]GT
ApaI          313  GGGCCC               GGGCC^C
Asp700I       818  GAAATATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         370  GTGCTC               G[AT]GC[AT]^C
AviII         278  TGCGCA               TGC^GCA
BalI          290  TGGCCA               TGG^CCA
BanI          402  GGTGCC               G^G[CT][GA]CC
BanII         313  GGGCCC               G[GA]GC[CT]^C
BbsI          653  GTCTTC               GAAGAC(2/6)
BfaI         1073  CTAG                 C^TAG
BpuAI         653  GTCTTC               GAAGAC(2/6)
BsiEI          29  CGGCCG               CG[GA][CT]^CG
BsiHKAI       370  GTGCTC               G[AT]GC[AT]^C
BsmAI          76  GAGAC                GTCTC(1/5)
BsmBI          76  GAGACG               CGTCTC(1/5)
BsmFI          54  GTCCC                GGGAC(10/14)
BsmI         1091  GAATGC               GAATGC(1/-1)
BsrFI         302  ACCGGC               [GA]^CCGG[CT]
BstBI         815  TTCGAA               TT^CGAA
BstF5I        471  CATCC                GGATG(2/0)
BstXI         329  CCAAGGGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         689  AGATCC               [GA]^GATC[CT]
Cfr10I        302  ACCGGC               [GA]^CCGG[CT]
DraII         332  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DsaI          178  CCACGG               C^C[GA][CT]GG
EagI           29  CGGCCG               C^GGCCG
EarI          712  GAAGAG               CTCTTC(1/4)
EclXI          29  CGGCCG               C^GGCCG
Eco52I         29  CGGCCG               C^GGCCG
EcoO109I      332  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       329  CCAAGG               C^C[AT][AT]GG
FokI          471  CATCC                GGATG(9/13)
FspI          278  TGCGCA               TGC^GCA
HgaI           78  GACGC                GACGC(5/10)
HincII        237  GTTGAC               GT[CT]^[GA]AC
HindII        237  GTTGAC               GT[CT]^[GA]AC
HphI          994  TCACC                GGTGA(8/7)
Ksp632I       712  GAAGAG               CTCTTC(1/4)
MaeI         1073  CTAG                 C^TAG
MaeII         221  ACGT                 A^CGT
MfeI          882  CAATTG               C^AATTG
MflI          689  AGATCC               [GA]^GATC[CT]
MluI          233  ACGCGT               A^CGCGT
MluNI         290  TGGCCA               TGG^CCA
MscI          290  TGGCCA               TGG^CCA
MslI          995  CACCAGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          882  CAATTG               C^AATTG
NspV          815  TTCGAA               TT^CGAA
PaeR7I        683  CTCGAG               C^TCGAG
PvuII         211  CAGCTG               CAG^CTG
SfiI           30  GGCCGGGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI          815  TTCGAA               TT^CGAA
SmlI          683  CTCGAG               C^T[CT][GA]AG
SspI          820  AATATT               AAT^ATT
StyI          329  CCAAGG               C^C[AT][AT]GG
XcmI           71  CCACCGAGACGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          683  CTCGAG               C^TCGAG
XhoII         689  AGATCC               [GA]^GATC[CT]
XmnI          818  GAAATATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcsI          525  AAATTT               [GA]^AATT[CT]
              940  AAATTT              
AlwI          690  GATCC                GGATC(4/5)
              772  GATCC               
AlwNI         211  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              436  CAGACCCTG           
ApoI          525  AAATTT               [GA]^AATT[CT]
              940  AAATTT              
AvaI          247  CTCGGG               C^[CT]CG[GA]G
              683  CTCGAG              
AvaII          53  GGTCC                G^G[AT]CC
              803  GGACC               
BbvCI          60  CCTCAGC              CCTCAGC(-5/-2)
              378  CCTCAGC             
BcnI           32  CCGGG                CC^[GC]GG
              474  CCCGG               
BglI           31  GCCGGGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              446  GCCTGGGCGGC         
BmyI          313  GGGCCC               G[AGT]GC[ACT]^C
              370  GTGCTC              
BsgI          797  CTGCAC               GTGCAG(16/14)
             1082  CTGCAC              
BsoBI         247  CTCGGG               C^[CT]CG[GA]G
              683  CTCGAG              
Bsp1286I      313  GGGCCC               G[AGT]GC[ACT]^C
              370  GTGCTC              
BssHII        426  GCGCGC               G^CGCGC
              428  GCGCGC              
DrdI           78  GACGCTGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              919  GACTGCTGTGTC        
EaeI           29  CGGCCG               [CT]^GGCC[GA]
              290  TGGCCA              
HaeII         132  GGCGCT               [GA]GCGC^[CT]
              183  GGCGCT              
NciI           32  CCGGG                CC^[GC]GG
              474  CCCGG               
PflMI         210  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              895  CCAAAGTGTGG         
PstI          433  CTGCAG               CTGCA^G
              463  CTGCAG              
SfaNI         978  GATGC                GCATC(5/9)
              470  GCATC               
SfcI          433  CTGCAG               C^T[GA][CT]AG
              463  CTGCAG              
TfiI          813  GATTC                G^A[AT]TC
             1051  GAATC               
Tsp45I         25  GTCAC                ^GT[GC]AC
              590  GTGAC               
TspRI         318  CAGTG                CA[GC]TG[ACGT][ACGT]^
              974  CACTG               
Van91I        210  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              895  CCAAAGTGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AccII       AciI        AluI        BbvI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BsrBI       
BsrI        BssKI       BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EcoRII      
FauI        Fnu4HI      HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       ItaI        MaeIII      
MboI        MboII       MnlI        MseI        MspA1I      
MspI        MvaI        MvnI        MwoI        NdeII       
NlaIV       PleI        Sau3AI      Sau96I      ScrFI       
TaqI        Tru9I       TseI        Tsp509I     TthHB8I     
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