Restriction map for pF1KE0208
 (Linear) (Six-base) MAPSORT of: KIEE0208.seq  Check: 7653  from: 1  to: 1113



KIEE0208.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AccI GT'mk_AC

Cuts at:      0     911    1113 
   Size:        911     202
 
AflIII A'CryG_T

Cuts at:      0     233    1113 
   Size:        233     880
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     478    1113 
   Size:        478     635
 
AlwNI CAG_nnn'CTG

Cuts at:      0     216     441    1113 
   Size:        216     225     672

  Fragments arranged by size: 

                 672     225     216
 
ApaI G_GGCC'C

Cuts at:      0     317    1113 
   Size:        317     796
 
ApoI r'AATT_y

Cuts at:      0     525     940    1113 
   Size:        525     415     173

  Fragments arranged by size: 

                 525     415     173
 
AvaI C'yCGr_G

Cuts at:      0     247     683    1113 
   Size:        247     436     430

  Fragments arranged by size: 

                 436     430     247
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     855     888    1113 
   Size:        855      33     225

  Fragments arranged by size: 

                 855     225      33
 
BanI G'GyrC_C

Cuts at:      0     402    1113 
   Size:        402     711
 
BanII G_rGCy'C

Cuts at:      0     317    1113 
   Size:        317     796
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     646    1113 
   Size:        646     467
 
BbvCI CC'TCA_GC

Cuts at:      0      61     379    1113 
   Size:         61     318     734

  Fragments arranged by size: 

                 734     318      61
 
BglI GCCn_nnn'nGGC

Cuts at:      0      37     452    1113 
   Size:         37     415     661

  Fragments arranged by size: 

                 661     415      37
 
Bme1580I G_kGCm'C

Cuts at:      0     317    1113 
   Size:        317     796
 
BmgBI CAC'GTC

Cuts at:      0     222    1113 
   Size:        222     891
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     311     336    1113 
   Size:        311      25     777

  Fragments arranged by size: 

                 777     311      25
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     709    1113 
   Size:        709     404
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     115     193     349    1113 
   Size:        103      12      78     156     764

  Fragments arranged by size: 

                 764     156     103      78      12
 
Bpu10I CC'TnA_GC

Cuts at:      0      61     379     442    1113 
   Size:         61     318      63     671

  Fragments arranged by size: 

                 671     318      63      61
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       5      35    1113 
   Size:          5      30    1078

  Fragments arranged by size: 

                1078      30       5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     124     605     920    1113 
   Size:         19     105     481     315     193

  Fragments arranged by size: 

                 481     315     193     105      19
 
BseYI C'CCAG_C

Cuts at:      0     186    1113 
   Size:        186     927
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     782    1067    1113 
   Size:        782     285      46

  Fragments arranged by size: 

                 782     285      46
 
BsiEI CG_ry'CG

Cuts at:      0      32    1113 
   Size:         32    1081
 
BsiHKAI G_wGCw'C

Cuts at:      0     374    1113 
   Size:        374     739
 
BsmI GAATG_Cn'

Cuts at:      0    1097    1113 
   Size:       1097      16
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      70    1113 
   Size:         70    1043
 
Bsp1286I G_dGCh'C

Cuts at:      0     317     374    1113 
   Size:        317      57     739

  Fragments arranged by size: 

                 739     317      57
 
BsrBI CCG'CTC

Cuts at:      0     114     227     351    1113 
   Size:        114     113     124     762

  Fragments arranged by size: 

                 762     124     114     113
 
BsrFI r'CCGG_y

Cuts at:      0     302    1113 
   Size:        302     811
 
BssHII G'CGCG_C

Cuts at:      0     426     428    1113 
   Size:        426       2     685

  Fragments arranged by size: 

                 685     426       2
 
BstBI TT'CG_AA

Cuts at:      0     816    1113 
   Size:        816     297
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     336    1113 
   Size:        336     777
 
BstYI r'GATC_y

Cuts at:      0     689    1113 
   Size:        689     424
 
BtgI C'CryG_G

Cuts at:      0     178    1113 
   Size:        178     935
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      84     925    1113 
   Size:         84     841     188

  Fragments arranged by size: 

                 841     188      84
 
EaeI y'GGCC_r

Cuts at:      0      29     290    1113 
   Size:         29     261     823

  Fragments arranged by size: 

                 823     261      29
 
EagI C'GGCC_G

Cuts at:      0      29    1113 
   Size:         29    1084
 
EarI CTCTTCn'nnn_

Cuts at:      0     707    1113 
   Size:        707     406
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     115     193     349    1113 
   Size:        103      12      78     156     764

  Fragments arranged by size: 

                 764     156     103      78      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0     333    1113 
   Size:        333     780
 
FspI TGC'GCA

Cuts at:      0     280    1113 
   Size:        280     833
 
HaeII r_GCGC'y

Cuts at:      0     136     187    1113 
   Size:        136      51     926

  Fragments arranged by size: 

                 926     136      51
 
HincII GTy'rAC

Cuts at:      0     239    1113 
   Size:        239     874
 
MfeI C'AATT_G

Cuts at:      0     882    1113 
   Size:        882     231
 
MluI A'CGCG_T

Cuts at:      0     233    1113 
   Size:        233     880
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     802    1113 
   Size:        802     311
 
MscI TGG'CCA

Cuts at:      0     292    1113 
   Size:        292     821
 
MslI CAynn'nnrTG

Cuts at:      0     999    1113 
   Size:        999     114
 
MspA1I CmG'CkG

Cuts at:      0      19     194     213     383    1113 
   Size:         19     175      19     170     730

  Fragments arranged by size: 

                 730     175     170      19      19
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     216     901    1113 
   Size:        216     685     212

  Fragments arranged by size: 

                 685     216     212
 
PfoI T'CCnGG_A

Cuts at:      0     395     785     846    1113 
   Size:        395     390      61     267

  Fragments arranged by size: 

                 395     390     267      61
 
PspOMI G'GGCC_C

Cuts at:      0     313    1113 
   Size:        313     800
 
PstI C_TGCA'G

Cuts at:      0     437     467    1113 
   Size:        437      30     646

  Fragments arranged by size: 

                 646     437      30
 
PvuII CAG'CTG

Cuts at:      0     213    1113 
   Size:        213     900
 
SfcI C'TryA_G

Cuts at:      0     433     463    1113 
   Size:        433      30     650

  Fragments arranged by size: 

                 650     433      30
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      37    1113 
   Size:         37    1076
 
SmlI C'TyrA_G

Cuts at:      0     683    1113 
   Size:        683     430
 
SspI AAT'ATT

Cuts at:      0     822    1113 
   Size:        822     291
 
StyI C'CwwG_G

Cuts at:      0     329    1113 
   Size:        329     784
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       5      37    1113 
   Size:          5      32    1076

  Fragments arranged by size: 

                1076      32       5
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      78    1113 
   Size:         78    1035
 
XhoI C'TCGA_G

Cuts at:      0     683    1113 
   Size:        683     430
 
XmnI GAAnn'nnTTC

Cuts at:      0     822    1113 
   Size:        822     291
 

 Enzymes that do cut:

     AccI   AflIII     AlfI    AlwNI     ApaI     ApoI     AvaI     BaeI
     BanI    BanII     BbsI    BbvCI     BglI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BsrBI    BsrFI   BssHII    BstBI
    BstXI    BstYI     BtgI     DrdI     EaeI     EagI     EarI  Eco57MI
 EcoO109I     FspI    HaeII   HincII     MfeI     MluI     MmeI     MscI
     MslI   MspA1I    PflMI     PfoI   PspOMI     PstI    PvuII     SfcI
     SfiI     SmlI     SspI     StyI     TstI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
    BamHI     BbeI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
     BmtI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsiWI
    BspEI    BspHI    BspMI    BsrDI    BsrGI    BssSI   BstAPI   BstEII
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI
    Hin4I  HindIII     HpaI     KasI     KpnI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TatI
  Tth111I     XbaI     XmaI     ZraI
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