Restriction map for pF1KE0449
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AfaI        AflII       AflIII      AgeI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbsI        
BcgI        BciVI       BclI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EaeI        EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HaeII       
HincII      HindII      HpaI        KasI        KpnI        
KspI        MaeIII      MamI        MfeI        MluI        
MluNI       MroI        MscI        MslI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        Tsp45I      Tsp509I     Tth111I     TthHB8I     
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          281  GTAGAC               GT^[AC][GT]AC
AccII          13  CGCG                 CG^CG
AhdI          284  GACGCCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         284  GACGCCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII         188  GAGCTC               G[GA]GC[CT]^C
BbvCI         747  GCTGAGG              CCTCAGC(-5/-2)
BcnI          740  CCGGG                CC^[GC]GG
BglII         803  AGATCT               A^GATCT
BsaI          341  GGTCTC               GGTCTC(1/5)
BseRI         532  GAGGAG               GAGGAG(10/8)
BsmAI         342  GTCTC                GTCTC(1/5)
BspMI         560  ACCTGC               ACCTGC(4/8)
BsrBI         582  GAGCGG               CCGCTC(-3/-3)
BstAPI        701  GCAGCCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          13  CGCG                 CG^CG
BstYI         803  AGATCT               [GA]^GATC[CT]
DpnII         804  GATC                 ^GATC
DsaI          612  CCGTGG               C^C[GA][CT]GG
Eam1105I      284  GACGCCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI           95  CTCTTC               CTCTTC(1/4)
EcoNI          94  CCTCTTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII         740  CCGG                 C^CGG
HindIII       141  AAGCTT               A^AGCTT
HpaII         740  CCGG                 C^CGG
Ksp632I        95  CTCTTC               CTCTTC(1/4)
MaeII         380  ACGT                 A^CGT
MboI          804  GATC                 ^GATC
MflI          803  AGATCT               [GA]^GATC[CT]
MseI          301  TTAA                 T^TAA
MspA1I         85  CAGCTG               C[AC]G^C[GT]G
MspI          740  CCGG                 C^CGG
MvnI           13  CGCG                 CG^CG
NciI          740  CCGGG                CC^[GC]GG
NdeII         804  GATC                 ^GATC
NspI          134  ACATGC               [GA]CATG^[CT]
PflMI         471  CCAAGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII          85  CAGCTG               CAG^CTG
SacI          188  GAGCTC               GAGCT^C
Sau3AI        804  GATC                 ^GATC
SexAI         108  ACCAGGT              A^CC[AT]GGT
TfiI          241  GAATC                G^A[AT]TC
Tru9I         301  TTAA                 T^TAA
Van91I        471  CCAAGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         803  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcyI           49  GGCGTC               G[GA]^CG[CT]C
              284  GACGCC              
AlwNI         239  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
              702  CAGCCCCTG           
AspHI         188  GAGCTC               G[AT]GC[AT]^C
              588  GAGCAC              
AvaI           66  CTCGGG               C^[CT]CG[GA]G
              627  CTCGGG              
AvaII         652  GGACC                G^G[AT]CC
              754  GGTCC               
BmyI          188  GAGCTC               G[AGT]GC[ACT]^C
              588  GAGCAC              
BpmI          712  CTCCAG               CTGGAG(16/14)
              478  CTGGAG              
BsaHI          49  GGCGTC               G[GA]^CG[CT]C
              284  GACGCC              
BsiHKAI       188  GAGCTC               G[AT]GC[AT]^C
              588  GAGCAC              
BsoBI          66  CTCGGG               C^[CT]CG[GA]G
              627  CTCGGG              
Bsp1286I      188  GAGCTC               G[AGT]GC[ACT]^C
              588  GAGCAC              
BsrI          477  ACTGG                ACTGG(1/-1)
              365  CCAGT               
BstXI         390  CCAGCAGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              783  CCAGCAGGATGG        
FauI          545  CCCGC                CCCGC(4/6)
              584  GCGGG               
HaeIII        541  GGCC                 GG^CC
              673  GGCC                
HgaI          284  GACGC                GACGC(5/10)
               50  GCGTC               
Hin1I          49  GGCGTC               G[GA]^CG[CT]C
              284  GACGCC              
HphI          259  GGTGA                GGTGA(8/7)
              687  GGTGA               
PpuMI         651  GGGACCC              [GA]G^G[AT]CC[CT]
              753  GGGTCCT             
XbaI          345  TCTAGA               T^CTAGA
              456  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AciI        AluI        BbvI        BfaI        BsaJI       
BsgI        BsiYI       BslI        BsmFI       BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DraII       EcoO109I    EcoRII      Fnu4HI      
FokI        HhaI        HinP1I      HinfI       ItaI        
MaeI        MboII       MnlI        MvaI        MwoI        
NlaIII      NlaIV       PleI        PstI        Sau96I      
ScrFI       SfcI        TseI        TspRI       
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