Restriction map for pF1KE0449
 (Linear) (Six-base) MAPSORT of: KIEE0449.seq  Check: 2144  from: 1  to: 813



KIEE0449.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AccI GT'mk_AC

Cuts at:      0     282     813 
   Size:        282     531
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     200     469     534     813 
   Size:         41     159     269      65     279

  Fragments arranged by size: 

                 279     269     159      65      41
 
AhdI GACnn_n'nnGTC

Cuts at:      0     289     813 
   Size:        289     524
 
AlwNI CAG_nnn'CTG

Cuts at:      0     244     707     813 
   Size:        244     463     106

  Fragments arranged by size: 

                 463     244     106
 
AvaI C'yCGr_G

Cuts at:      0      66     627     813 
   Size:         66     561     186

  Fragments arranged by size: 

                 561     186      66
 
BanII G_rGCy'C

Cuts at:      0     192     813 
   Size:        192     621
 
BbvCI CC'TCA_GC

Cuts at:      0     748     813 
   Size:        748      65
 
BglII A'GATC_T

Cuts at:      0     803     813 
   Size:        803      10
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     499     697     813 
   Size:        499     198     116

  Fragments arranged by size: 

                 499     198     116
 
Bpu10I CC'TnA_GC

Cuts at:      0     748     813 
   Size:        748      65
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     347     813 
   Size:        347     466
 
BsaHI Gr'CG_yC

Cuts at:      0      50     285     813 
   Size:         50     235     528

  Fragments arranged by size: 

                 528     235      50
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     547     813 
   Size:        547     266
 
BseYI C'CCAG_C

Cuts at:      0     397     813 
   Size:        397     416
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      18     627     640     813 
   Size:         18     609      13     173

  Fragments arranged by size: 

                 609     173      18      13
 
BsiHKAI G_wGCw'C

Cuts at:      0     192     592     813 
   Size:        192     400     221

  Fragments arranged by size: 

                 400     221     192
 
Bsp1286I G_dGCh'C

Cuts at:      0     192     592     813 
   Size:        192     400     221

  Fragments arranged by size: 

                 400     221     192
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     569     813 
   Size:        569     244
 
BsrBI CCG'CTC

Cuts at:      0     584     813 
   Size:        584     229
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     707     813 
   Size:        707     106
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     397     790     813 
   Size:        397     393      23

  Fragments arranged by size: 

                 397     393      23
 
BstYI r'GATC_y

Cuts at:      0     803     813 
   Size:        803      10
 
BtgI C'CryG_G

Cuts at:      0     612     813 
   Size:        612     201
 
BtsI GCAGTG_nn'

Cuts at:      0     741     813 
   Size:        741      72
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     127     162     813 
   Size:        127      35     651

  Fragments arranged by size: 

                 651     127      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     101     813 
   Size:        101     712
 
EcoICRI GAG'CTC

Cuts at:      0     190     813 
   Size:        190     623
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     200     469     499     534     697     813 
   Size:         41     159     269      30      35     163     116

  Fragments arranged by size: 

                 269     163     159     116      41      35      30
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      98     813 
   Size:         98     715
 
EcoO109I rG'GnC_Cy

Cuts at:      0     541     652     754     813 
   Size:        541     111     102      59

  Fragments arranged by size: 

                 541     111     102      59
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     178     813 
   Size:        178     635
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     143     175     275     307     813 
   Size:        143      32     100      32     506

  Fragments arranged by size: 

                 506     143     100      32      32
 
HindIII A'AGCT_T

Cuts at:      0     141     813 
   Size:        141     672
 
MspA1I CmG'CkG

Cuts at:      0      87     813 
   Size:         87     726
 
NspI r_CATG'y

Cuts at:      0     138     813 
   Size:        138     675
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     477     813 
   Size:        477     336
 
PfoI T'CCnGG_A

Cuts at:      0     713     813 
   Size:        713     100
 
PpuMI rG'GwC_Cy

Cuts at:      0     652     754     813 
   Size:        652     102      59

  Fragments arranged by size: 

                 652     102      59
 
PstI C_TGCA'G

Cuts at:      0      86     729     736     813 
   Size:         86     643       7      77

  Fragments arranged by size: 

                 643      86      77       7
 
PvuII CAG'CTG

Cuts at:      0      87     813 
   Size:         87     726
 
SacI G_AGCT'C

Cuts at:      0     192     813 
   Size:        192     621
 
SanDI GG'GwC_CC

Cuts at:      0     652     813 
   Size:        652     161
 
SexAI A'CCwGG_T

Cuts at:      0     108     813 
   Size:        108     705
 
SfcI C'TryA_G

Cuts at:      0      82     310     725     732     813 
   Size:         82     228     415       7      81

  Fragments arranged by size: 

                 415     228      82      81       7
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     482     514     813 
   Size:        482      32     299

  Fragments arranged by size: 

                 482     299      32
 
XbaI T'CTAG_A

Cuts at:      0     345     456     813 
   Size:        345     111     357

  Fragments arranged by size: 

                 357     345     111
 

 Enzymes that do cut:

     AccI     AcuI     AhdI    AlwNI     AvaI    BanII    BbvCI    BglII
     BpmI   Bpu10I     BsaI    BsaHI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspMI    BsrBI   BstAPI    BstXI    BstYI     BtgI     BtsI
    CspCI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    Hin4I
  HindIII   MspA1I     NspI    PflMI     PfoI    PpuMI     PstI    PvuII
     SacI    SanDI    SexAI     SfcI     TstI     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI
     DraI   DraIII     DrdI     EaeI     EagI     EciI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI
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