Restriction map for pF1KE0375
 (Linear) (Six-base) MAPSORT of: KIEE0375.seq  Check: 6483  from: 1  to: 1701



KIEE0375.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     366    1701 
   Size:        366    1335
 
AatII G_ACGT'C

Cuts at:      0     203    1701 
   Size:        203    1498
 
AccI GT'mk_AC

Cuts at:      0    1354    1701 
   Size:       1354     347
 
Acc65I G'GTAC_C

Cuts at:      0    1535    1629    1701 
   Size:       1535      94      72

  Fragments arranged by size: 

                1535      94      72
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     516     676    1451    1701 
   Size:        516     160     775     250

  Fragments arranged by size: 

                 775     516     250     160
 
AgeI A'CCGG_T

Cuts at:      0     963    1701 
   Size:        963     738
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1048    1080    1701 
   Size:       1048      32     621

  Fragments arranged by size: 

                1048     621      32
 
ApaI G_GGCC'C

Cuts at:      0    1079    1701 
   Size:       1079     622
 
ApoI r'AATT_y

Cuts at:      0     397     770    1249    1283    1429    1701 
   Size:        397     373     479      34     146     272

  Fragments arranged by size: 

                 479     397     373     272     146      34
 
AvaI C'yCGr_G

Cuts at:      0     605    1701 
   Size:        605    1096
 
BanI G'GyrC_C

Cuts at:      0      45     160     667    1535    1629    1701 
   Size:         45     115     507     868      94      72

  Fragments arranged by size: 

                 868     507     115      94      72      45
 
BanII G_rGCy'C

Cuts at:      0    1079    1592    1701 
   Size:       1079     513     109

  Fragments arranged by size: 

                1079     513     109
 
BbeI G_GCGC'C

Cuts at:      0     164    1701 
   Size:        164    1537
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     195    1701 
   Size:        195    1506
 
BbvCI CC'TCA_GC

Cuts at:      0     298    1701 
   Size:        298    1403
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1294    1390    1701 
   Size:       1294      96     311

  Fragments arranged by size: 

                1294     311      96
 
BglII A'GATC_T

Cuts at:      0    1131    1701 
   Size:       1131     570
 
Bme1580I G_kGCm'C

Cuts at:      0      50    1079    1701 
   Size:         50    1029     622

  Fragments arranged by size: 

                1029     622      50
 
BmgBI CAC'GTC

Cuts at:      0     312    1701 
   Size:        312    1389
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     474     798    1701 
   Size:        474     324     903

  Fragments arranged by size: 

                 903     474     324
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     469     961     967    1701 
   Size:        469     492       6     734

  Fragments arranged by size: 

                 734     492     469       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     298    1701 
   Size:        298    1403
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1370    1701 
   Size:       1370     331
 
BsaAI yAC'GTr

Cuts at:      0     740    1701 
   Size:        740     961
 
BsaHI Gr'CG_yC

Cuts at:      0     161     200    1701 
   Size:        161      39    1501

  Fragments arranged by size: 

                1501     161      39
 
BsaWI w'CCGG_w

Cuts at:      0     963    1701 
   Size:        963     738
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     313     343     467     497    1048    1078    1664    1694 
   Size:        313      30     124      30     551      30     586      30

Cuts at:   1694    1701 
   Size:          7

  Fragments arranged by size: 

                 586     551     313     124      30      30      30      30
                   7
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1672    1701 
   Size:       1672      29
 
BseYI C'CCAG_C

Cuts at:      0      54    1078    1701 
   Size:         54    1024     623

  Fragments arranged by size: 

                1024     623      54
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     953    1701 
   Size:        292     661     748

  Fragments arranged by size: 

                 748     661     292
 
BsiEI CG_ry'CG

Cuts at:      0    1095    1701 
   Size:       1095     606
 
BsmI GAATG_Cn'

Cuts at:      0     723    1701 
   Size:        723     978
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     235     625    1701 
   Size:        235     390    1076

  Fragments arranged by size: 

                1076     390     235
 
Bsp1286I G_dGCh'C

Cuts at:      0      50    1079    1592    1701 
   Size:         50    1029     513     109

  Fragments arranged by size: 

                1029     513     109      50
 
BspHI T'CATG_A

Cuts at:      0     905    1701 
   Size:        905     796
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     366    1701 
   Size:        366    1335
 
BsrDI GCAATG_nn'

Cuts at:      0     617    1493    1701 
   Size:        617     876     208

  Fragments arranged by size: 

                 876     617     208
 
BsrFI r'CCGG_y

Cuts at:      0     670     886     963    1701 
   Size:        670     216      77     738

  Fragments arranged by size: 

                 738     670     216      77
 
BsrGI T'GTAC_A

Cuts at:      0     677    1701 
   Size:        677    1024
 
BstEII G'GTnAC_C

Cuts at:      0     959    1701 
   Size:        959     742
 
BstYI r'GATC_y

Cuts at:      0     468    1131    1701 
   Size:        468     663     570

  Fragments arranged by size: 

                 663     570     468
 
BstZ17I GTA'TAC

Cuts at:      0    1355    1701 
   Size:       1355     346
 
Bsu36I CC'TnA_GG

Cuts at:      0    1687    1701 
   Size:       1687      14
 
BtgI C'CryG_G

Cuts at:      0     663    1162    1701 
   Size:        663     499     539

  Fragments arranged by size: 

                 663     539     499
 
BtsI GCAGTG_nn'

Cuts at:      0    1661    1701 
   Size:       1661      40
 
ClaI AT'CG_AT

Cuts at:      0     416    1701 
   Size:        416    1285
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     480     515    1505    1540    1701 
   Size:        480      35     990      35     161

  Fragments arranged by size: 

                 990     480     161      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     214     740    1701 
   Size:        214     526     961

  Fragments arranged by size: 

                 961     526     214
 
EaeI y'GGCC_r

Cuts at:      0     521    1126    1701 
   Size:        521     605     575

  Fragments arranged by size: 

                 605     575     521
 
EarI CTCTTCn'nnn_

Cuts at:      0      77     979    1701 
   Size:         77     902     722

  Fragments arranged by size: 

                 902     722      77
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     469     516     676     961     967    1451    1701 
   Size:        469      47     160     285       6     484     250

  Fragments arranged by size: 

                 484     469     285     250     160      47       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     679    1701 
   Size:        679    1022
 
EcoO109I rG'GnC_Cy

Cuts at:      0      74     184     822    1075    1701 
   Size:         74     110     638     253     626

  Fragments arranged by size: 

                 638     626     253     110      74
 
EcoRI G'AATT_C

Cuts at:      0     397    1283    1701 
   Size:        397     886     418

  Fragments arranged by size: 

                 886     418     397
 
HaeII r_GCGC'y

Cuts at:      0     164    1701 
   Size:        164    1537
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1123    1155    1701 
   Size:       1123      32     546

  Fragments arranged by size: 

                1123     546      32
 
HincII GTy'rAC

Cuts at:      0     315    1116    1701 
   Size:        315     801     585

  Fragments arranged by size: 

                 801     585     315
 
HindIII A'AGCT_T

Cuts at:      0    1544    1701 
   Size:       1544     157
 
KasI G'GCGC_C

Cuts at:      0     160    1701 
   Size:        160    1541
 
KpnI G_GTAC'C

Cuts at:      0    1539    1633    1701 
   Size:       1539      94      68

  Fragments arranged by size: 

                1539      94      68
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1352    1701 
   Size:       1352     349
 
MscI TGG'CCA

Cuts at:      0     523    1128    1701 
   Size:        523     605     573

  Fragments arranged by size: 

                 605     573     523
 
MslI CAynn'nnrTG

Cuts at:      0    1222    1609    1701 
   Size:       1222     387      92

  Fragments arranged by size: 

                1222     387      92
 
MspA1I CmG'CkG

Cuts at:      0     480     665    1701 
   Size:        480     185    1036

  Fragments arranged by size: 

                1036     480     185
 
NarI GG'CG_CC

Cuts at:      0     161    1701 
   Size:        161    1540
 
NcoI C'CATG_G

Cuts at:      0    1162    1701 
   Size:       1162     539
 
NspI r_CATG'y

Cuts at:      0    1608    1701 
   Size:       1608      93
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     719    1701 
   Size:        719     982
 
PfoI T'CCnGG_A

Cuts at:      0     278     977    1701 
   Size:        278     699     724

  Fragments arranged by size: 

                 724     699     278
 
PmlI CAC'GTG

Cuts at:      0     740    1701 
   Size:        740     961
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      96     128    1048    1080    1701 
   Size:         96      32     920      32     621

  Fragments arranged by size: 

                 920     621      96      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      74     822    1701 
   Size:         74     748     879

  Fragments arranged by size: 

                 879     748      74
 
PsiI TTA'TAA

Cuts at:      0     731    1701 
   Size:        731     970
 
PspOMI G'GGCC_C

Cuts at:      0    1075    1701 
   Size:       1075     626
 
PstI C_TGCA'G

Cuts at:      0    1587    1701 
   Size:       1587     114
 
PvuI CG_AT'CG

Cuts at:      0    1095    1701 
   Size:       1095     606
 
PvuII CAG'CTG

Cuts at:      0     480    1701 
   Size:        480    1221
 
SacII CC_GC'GG

Cuts at:      0     666    1701 
   Size:        666    1035
 
SanDI GG'GwC_CC

Cuts at:      0      74    1701 
   Size:         74    1627
 
SapI GCTCTTCn'nnn_

Cuts at:      0     979    1701 
   Size:        979     722
 
SfcI C'TryA_G

Cuts at:      0     402     847    1583    1701 
   Size:        402     445     736     118

  Fragments arranged by size: 

                 736     445     402     118
 
SfoI GGC'GCC

Cuts at:      0     162    1701 
   Size:        162    1539
 
SspI AAT'ATT

Cuts at:      0    1477    1701 
   Size:       1477     224
 
StuI AGG'CCT

Cuts at:      0      88     562     944    1701 
   Size:         88     474     382     757

  Fragments arranged by size: 

                 757     474     382      88
 
StyI C'CwwG_G

Cuts at:      0     818    1162    1701 
   Size:        818     344     539

  Fragments arranged by size: 

                 818     539     344
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     491    1701 
   Size:        491    1210
 
TatI w'GTAC_w

Cuts at:      0     677    1258    1701 
   Size:        677     581     443

  Fragments arranged by size: 

                 677     581     443
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     467     499    1701 
   Size:        467      32    1202

  Fragments arranged by size: 

                1202     467      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1224    1701 
   Size:       1224     477
 
ZraI GAC'GTC

Cuts at:      0     201    1701 
   Size:        201    1500
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AgeI     AloI     ApaI
     ApoI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI
    BglII Bme1580I    BmgBI     BmrI     BpmI   Bpu10I     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BstEII
    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI   DraIII
     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MmeI     MscI     MslI   MspA1I
     NarI     NcoI     NspI    PflMI     PfoI     PmlI     PpiI    PpuMI
     PsiI   PspOMI     PstI     PvuI    PvuII    SacII    SanDI     SapI
     SfcI     SfoI     SspI     StuI     StyI    TaqII     TatI     TstI
     XcmI     ZraI

 Enzymes that do not cut: 

     AclI     AfeI    AflII   AflIII     AhdI     AleI     AlfI    AlwNI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BcgI
     BclI    BfrBI     BglI     BlpI     BmtI     BplI    BpuEI    BsaBI
  BsiHKAI    BsiWI    BspEI    BsrBI   BssHII    BssSI   BstAPI    BstBI
    BstXI    BtgZI     DraI     DrdI     EagI     EciI  EcoICRI    EcoRV
     FalI     FseI     FspI    FspAI     HpaI     MfeI     MluI     NaeI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PmeI    PshAI    PspXI     PsrI    RsrII     SacI     SalI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SwaI  Tth111I     XbaI     XhoI
     XmaI     XmnI
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