Restriction map for pF1KE0048
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       AccII       AccIII      AciI        AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     AspEI       AspHI       
AspI        AviII       AvrII       BbeI        BbrPI       
BbsI        BbvI        BcgI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        BspDI       BspEI       BspHI       
BspMI       BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        Fnu4HI      FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HindIII     HpaI        ItaI        KasI        KspI        
MamI        MfeI        MluI        MroI        MspA1I      
MunI        MvnI        NaeI        NarI        NcoI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    StuI        
SwaI        TseI        Tth111I     XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

Acc65I        234  GGTACC               G^GTACC
AccI         1080  GTCTAC               GT^[AC][GT]AC
AflIII       1409  ACATGT               A^C[GA][CT]GT
Asp718I       234  GGTACC               G^GTACC
AvaI         1415  CTCGGG               C^[CT]CG[GA]G
AvaII         160  GGTCC                G^G[AT]CC
BamHI         283  GGATCC               G^GATCC
BanII        1026  GAGCCC               G[GA]GC[CT]^C
BbvCI          54  CCTCAGC              CCTCAGC(-5/-2)
BciVI         621  GTATCC               GTATCC(6/5)
BfaI         1070  CTAG                 C^TAG
BmyI         1026  GAGCCC               G[AGT]GC[ACT]^C
BpmI         1385  CTGGAG               CTGGAG(16/14)
BsmFI        1174  GTCCC                GGGAC(10/14)
BsoBI        1415  CTCGGG               C^[CT]CG[GA]G
Bsp1286I     1026  GAGCCC               G[AGT]GC[ACT]^C
Bsp1407I      612  TGTACA               T^GTACA
BspLU11I     1409  ACATGT               A^CATGT
BsrGI         612  TGTACA               T^GTACA
BstXI         874  CCAGAAAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I        1140  GCCAGC               GC[ACGT]^[ACGT]GC
DraII         894  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I      894  AGGCCCT              [GA]G^G[ACGT]CC[CT]
HincII        305  GTCAAC               GT[CT]^[GA]AC
HindII        305  GTCAAC               GT[CT]^[GA]AC
KpnI          234  GGTACC               GGTAC^C
MaeI         1070  CTAG                 C^TAG
NdeI          518  CATATG               CA^TATG
NspI         1409  ACATGT               [GA]CATG^[CT]
PflMI        1006  CCAAGAATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfaNI        1555  GATGC                GCATC(5/9)
SmlI          297  CTCAAG               C^T[CT][GA]AG
SspBI         612  TGTACA               T^GTACA
SspI          671  AATATT               AAT^ATT
Tsp45I        125  GTGAC                ^GT[GC]AC
Van91I       1006  CCAAGAATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1069  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AcsI          690  AAATTT               [GA]^AATT[CT]
              999  AAATTC              
AlwNI         986  CAGAGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1166  CAGTGTCTG           
ApoI          690  AAATTT               [GA]^AATT[CT]
              999  AAATTC              
BanI          234  GGTACC               G^G[CT][GA]CC
             1402  GGCACC              
BclI          387  TGATCA               T^GATCA
              549  TGATCA              
BcnI          141  CCGGG                CC^[GC]GG
              287  CCCGG               
BseRI         899  CTCCTC               GAGGAG(10/8)
             1388  GAGGAG              
BsmAI         701  GAGAC                GTCTC(1/5)
             1413  GTCTC               
BsrDI         638  CATTGC               GCAATG(2/0)
             1063  GCAATG              
BstYI         283  GGATCC               [GA]^GATC[CT]
             1479  GGATCT              
Cfr13I        160  GGTCC                G^G[ACGT]CC
              895  GGCCC               
DraIII         17  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
              905  CACGCAGTG           
EcoT14I        60  CCAAGG               C^C[AT][AT]GG
             1111  CCTTGG              
FbaI          387  TGATCA               T^GATCA
              549  TGATCA              
HapII         141  CCGG                 C^CGG
              288  CCGG                
HgaI           50  GCGTC                GACGC(5/10)
              660  GCGTC               
HpaII         141  CCGG                 C^CGG
              288  CCGG                
HphI          166  GGTGA                GGTGA(8/7)
              538  GGTGA               
MflI          283  GGATCC               [GA]^GATC[CT]
             1479  GGATCT              
MslI          244  CACTTAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1404  CACCCACATG          
MspI          141  CCGG                 C^CGG
              288  CCGG                
MwoI           50  GCGTCCTCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1019  GCCCATTGAGC         
NciI          141  CCGGG                CC^[GC]GG
              287  CCCGG               
NlaIII        535  CATG                 CATG^
             1410  CATG                
Sau96I        160  GGTCC                G^G[ACGT]CC
              895  GGCCC               
StyI           60  CCAAGG               C^C[AT][AT]GG
             1111  CCTTGG              
TfiI         1162  GAATC                G^A[AT]TC
             1303  GATTC               
XhoII         283  GGATCC               [GA]^GATC[CT]
             1479  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AfaI        AluI        AlwI        BalI        BsaJI       
BsiYI       BslI        BsrI        BssKI       BstF5I      
BstNI       DdeI        DpnII       EaeI        EarI        
EcoRII      FokI        HaeIII      HinfI       Ksp632I     
MaeII       MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MseI        MvaI        NdeII       
NlaIV       PleI        RsaI        Sau3AI      ScrFI       
TaqI        Tru9I       Tsp509I     TspRI       TthHB8I     
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