Restriction map for pF1KE0048
 (Linear) (Six-base) MAPSORT of: KIEE0048.seq  Check: 9383  from: 1  to: 1566



KIEE0048.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AccI GT'mk_AC

Cuts at:      0    1081    1566 
   Size:       1081     485
 
Acc65I G'GTAC_C

Cuts at:      0     234    1566 
   Size:        234    1332
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     663     838    1190    1199    1566 
   Size:        332     331     175     352       9     367

  Fragments arranged by size: 

                 367     352     332     331     175       9
 
AflIII A'CryG_T

Cuts at:      0    1409    1566 
   Size:       1409     157
 
AlwNI CAG_nnn'CTG

Cuts at:      0     991    1171    1566 
   Size:        991     180     395

  Fragments arranged by size: 

                 991     395     180
 
ApoI r'AATT_y

Cuts at:      0     690     999    1566 
   Size:        690     309     567

  Fragments arranged by size: 

                 690     567     309
 
AvaI C'yCGr_G

Cuts at:      0    1415    1566 
   Size:       1415     151
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     596     604     629     637    1566 
   Size:        596       8      25       8     929

  Fragments arranged by size: 

                 929     596      25       8       8
 
BamHI G'GATC_C

Cuts at:      0     283    1566 
   Size:        283    1283
 
BanI G'GyrC_C

Cuts at:      0     234    1402    1566 
   Size:        234    1168     164

  Fragments arranged by size: 

                1168     234     164
 
BanII G_rGCy'C

Cuts at:      0    1030    1566 
   Size:       1030     536
 
BbvCI CC'TCA_GC

Cuts at:      0      55    1566 
   Size:         55    1511
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     632    1566 
   Size:        632     934
 
BclI T'GATC_A

Cuts at:      0     387     549    1566 
   Size:        387     162    1017

  Fragments arranged by size: 

                1017     387     162
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1141    1566 
   Size:       1141     425
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1406    1566 
   Size:       1406     160
 
Bpu10I CC'TnA_GC

Cuts at:      0      55    1566 
   Size:         55    1511
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     282    1566 
   Size:        282    1284
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     890    1403    1566 
   Size:        890     513     163

  Fragments arranged by size: 

                 890     513     163
 
Bsp1286I G_dGCh'C

Cuts at:      0    1030    1566 
   Size:       1030     536
 
BsrDI GCAATG_nn'

Cuts at:      0     637    1070    1566 
   Size:        637     433     496

  Fragments arranged by size: 

                 637     496     433
 
BsrGI T'GTAC_A

Cuts at:      0     612    1566 
   Size:        612     954
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     881    1566 
   Size:        881     685
 
BstYI r'GATC_y

Cuts at:      0     283    1479    1566 
   Size:        283    1196      87

  Fragments arranged by size: 

                1196     283      87
 
BtsI GCAGTG_nn'

Cuts at:      0     915    1566 
   Size:        915     651
 
DraIII CAC_nnn'GTG

Cuts at:      0      22     910    1566 
   Size:         22     888     656

  Fragments arranged by size: 

                 888     656      22
 
EaeI y'GGCC_r

Cuts at:      0      10     883    1536    1566 
   Size:         10     873     653      30

  Fragments arranged by size: 

                 873     653      30      10
 
EarI CTCTTCn'nnn_

Cuts at:      0      92     450     468    1244    1566 
   Size:         92     358      18     776     322

  Fragments arranged by size: 

                 776     358     322      92      18
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     663     838    1190    1199    1406    1566 
   Size:        332     331     175     352       9     207     160

  Fragments arranged by size: 

                 352     332     331     207     175     160       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0     895    1566 
   Size:        895     671
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     658    1246    1566 
   Size:        658     588     320

  Fragments arranged by size: 

                 658     588     320
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     178     210     930     962    1087    1119    1338    1370 
   Size:        178      32     720      32     125      32     219      32

Cuts at:   1370    1566 
   Size:        196

  Fragments arranged by size: 

                 720     219     196     178     125      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     307    1566 
   Size:        307    1259
 
KpnI G_GTAC'C

Cuts at:      0     238    1566 
   Size:        238    1328
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     340    1566 
   Size:        340    1226
 
MscI TGG'CCA

Cuts at:      0      12     885    1538    1566 
   Size:         12     873     653      28

  Fragments arranged by size: 

                 873     653      28      12
 
MslI CAynn'nnrTG

Cuts at:      0     248    1408    1566 
   Size:        248    1160     158

  Fragments arranged by size: 

                1160     248     158
 
NdeI CA'TA_TG

Cuts at:      0     519    1566 
   Size:        519    1047
 
NspI r_CATG'y

Cuts at:      0    1413    1566 
   Size:       1413     153
 
PciI A'CATG_T

Cuts at:      0    1409    1566 
   Size:       1409     157
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1012    1566 
   Size:       1012     554
 
PfoI T'CCnGG_A

Cuts at:      0     286    1383    1566 
   Size:        286    1097     183

  Fragments arranged by size: 

                1097     286     183
 
SmlI C'TyrA_G

Cuts at:      0     297    1566 
   Size:        297    1269
 
SspI AAT'ATT

Cuts at:      0     673    1566 
   Size:        673     893
 
StyI C'CwwG_G

Cuts at:      0      60    1111    1566 
   Size:         60    1051     455

  Fragments arranged by size: 

                1051     455      60
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1420    1566 
   Size:       1420     146
 
TatI w'GTAC_w

Cuts at:      0     612    1566 
   Size:        612     954
 
XbaI T'CTAG_A

Cuts at:      0    1069    1566 
   Size:       1069     497
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI   AflIII    AlwNI     ApoI     AvaI     BaeI
    BamHI     BanI    BanII    BbvCI    BciVI     BclI     BmrI     BpmI
   Bpu10I    BpuEI    BseRI Bsp1286I    BsrDI    BsrGI    BstXI    BstYI
     BtsI   DraIII     EaeI     EarI  Eco57MI EcoO109I     FalI    Hin4I
   HincII     KpnI     MmeI     MscI     MslI     NdeI     NspI     PciI
    PflMI     PfoI     SmlI     SspI     StyI    TaqII     TatI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BbeI     BbsI     BcgI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     MfeI
     MluI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI     TstI  Tth111I     XcmI
     XhoI     XmaI     XmnI     ZraI
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