Restriction map for pF1KB8289
 (Linear) (Six-base) MAPSORT of: KIBB8289.seq  Check: 5228  from: 1  to: 906



KIBB8289.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     644     906 
   Size:        644     262
 
AflIII A'CryG_T

Cuts at:      0       5     906 
   Size:          5     901
 
AleI CACnn'nnGTG

Cuts at:      0     731     821     906 
   Size:        731      90      85

  Fragments arranged by size: 

                 731      90      85
 
AvaI C'yCGr_G

Cuts at:      0     299     906 
   Size:        299     607
 
BanI G'GyrC_C

Cuts at:      0     674     906 
   Size:        674     232
 
BanII G_rGCy'C

Cuts at:      0     140     277     431     797     906 
   Size:        140     137     154     366     109

  Fragments arranged by size: 

                 366     154     140     137     109
 
BbvCI CC'TCA_GC

Cuts at:      0     328     906 
   Size:        328     578
 
BglI GCCn_nnn'nGGC

Cuts at:      0     508     565     906 
   Size:        508      57     341

  Fragments arranged by size: 

                 508     341      57
 
BlpI GC'TnA_GC

Cuts at:      0     430     906 
   Size:        430     476
 
BmgBI CAC'GTC

Cuts at:      0       6     906 
   Size:          6     900
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     291     906 
   Size:        291     615
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     319     906 
   Size:        103     216     587

  Fragments arranged by size: 

                 587     216     103
 
Bpu10I CC'TnA_GC

Cuts at:      0     328     425     906 
   Size:        328      97     481

  Fragments arranged by size: 

                 481     328      97
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      82     148     313     906 
   Size:         82      66     165     593

  Fragments arranged by size: 

                 593     165      82      66
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     100     906 
   Size:        100     806
 
BseYI C'CCAG_C

Cuts at:      0     383     906 
   Size:        383     523
 
BsiHKAI G_wGCw'C

Cuts at:      0     277     431     754     797     906 
   Size:        277     154     323      43     109

  Fragments arranged by size: 

                 323     277     154     109      43
 
Bsp1286I G_dGCh'C

Cuts at:      0     140     277     431     754     797     906 
   Size:        140     137     154     323      43     109

  Fragments arranged by size: 

                 323     154     140     137     109      43
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     331     906 
   Size:        331     575
 
BsrFI r'CCGG_y

Cuts at:      0      55     339     906 
   Size:         55     284     567

  Fragments arranged by size: 

                 567     284      55
 
BssSI C'ACGA_G

Cuts at:      0     798     906 
   Size:        798     108
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     550     848     906 
   Size:        550     298      58

  Fragments arranged by size: 

                 550     298      58
 
BstYI r'GATC_y

Cuts at:      0     146     906 
   Size:        146     760
 
BtgI C'CryG_G

Cuts at:      0     248     470     906 
   Size:        248     222     436

  Fragments arranged by size: 

                 436     248     222
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     803     906 
   Size:        803     103
 
BtsI GCAGTG_nn'

Cuts at:      0     287     401     846     906 
   Size:        287     114     445      60

  Fragments arranged by size: 

                 445     287     114      60
 
EaeI y'GGCC_r

Cuts at:      0     257     341     500     906 
   Size:        257      84     159     406

  Fragments arranged by size: 

                 406     257     159      84
 
EarI CTCTTCn'nnn_

Cuts at:      0     265     354     640     889     906 
   Size:        265      89     286     249      17

  Fragments arranged by size: 

                 286     265     249      89      17
 
EcoICRI GAG'CTC

Cuts at:      0     275     429     795     906 
   Size:        275     154     366     111

  Fragments arranged by size: 

                 366     275     154     111
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     319     644     906 
   Size:        103     216     325     262

  Fragments arranged by size: 

                 325     262     216     103
 
HincII GTy'rAC

Cuts at:      0     375     906 
   Size:        375     531
 
HindIII A'AGCT_T

Cuts at:      0     216     906 
   Size:        216     690
 
MscI TGG'CCA

Cuts at:      0     502     906 
   Size:        502     404
 
MslI CAynn'nnrTG

Cuts at:      0     731     821     906 
   Size:        731      90      85

  Fragments arranged by size: 

                 731      90      85
 
MspA1I CmG'CkG

Cuts at:      0     550     659     906 
   Size:        550     109     247

  Fragments arranged by size: 

                 550     247     109
 
NaeI GCC'GGC

Cuts at:      0     341     906 
   Size:        341     565
 
NgoMIV G'CCGG_C

Cuts at:      0     339     906 
   Size:        339     567
 
NspI r_CATG'y

Cuts at:      0     107     632     906 
   Size:        107     525     274

  Fragments arranged by size: 

                 525     274     107
 
PfoI T'CCnGG_A

Cuts at:      0      29     906 
   Size:         29     877
 
PsiI TTA'TAA

Cuts at:      0     746     906 
   Size:        746     160
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     110     142     906 
   Size:        110      32     764

  Fragments arranged by size: 

                 764     110      32
 
PstI C_TGCA'G

Cuts at:      0     555     906 
   Size:        555     351
 
PvuII CAG'CTG

Cuts at:      0     550     906 
   Size:        550     356
 
SacI G_AGCT'C

Cuts at:      0     277     431     797     906 
   Size:        277     154     366     109

  Fragments arranged by size: 

                 366     277     154     109
 
SapI GCTCTTCn'nnn_

Cuts at:      0     265     354     906 
   Size:        265      89     552

  Fragments arranged by size: 

                 552     265      89
 
SfcI C'TryA_G

Cuts at:      0     127     551     807     906 
   Size:        127     424     256      99

  Fragments arranged by size: 

                 424     256     127      99
 
StyI C'CwwG_G

Cuts at:      0     606     906 
   Size:        606     300
 
XbaI T'CTAG_A

Cuts at:      0     354     906 
   Size:        354     552
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AvaI     BanI    BanII    BbvCI     BglI
     BlpI    BmgBI     BplI     BpmI   Bpu10I     BsaI    BseRI    BseYI
  BsiHKAI Bsp1286I    BspMI    BsrFI    BssSI   BstAPI    BstYI     BtgI
    BtgZI     BtsI     EaeI     EarI  EcoICRI  Eco57MI   HincII  HindIII
     MscI     MslI   MspA1I     NaeI   NgoMIV     NspI     PfoI     PsiI
     PsrI     PstI    PvuII     SacI     SapI     SfcI     StyI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI     BcgI
    BciVI     BclI    BfrBI    BglII Bme1580I     BmrI     BmtI    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XcmI
     XhoI     XmaI     XmnI     ZraI
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