Restriction map for
pF1KA2022
(Linear) (Six-base) MAPSORT of: KIAA2022.seq Check: 8982 from: 1 to: 4548
KIAA2022.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1878 4548
Size: 1878 2670
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 881 1084 1268 2044 2481 2726 4548
Size: 881 203 184 776 437 245 1822
Fragments arranged by size:
1822 881 776 437 245 203 184
AflII C'TTAA_G
Cuts at: 0 1261 3024 4548
Size: 1261 1763 1524
Fragments arranged by size:
1763 1524 1261
AflIII A'CryG_T
Cuts at: 0 526 881 4548
Size: 526 355 3667
Fragments arranged by size:
3667 526 355
AgeI A'CCGG_T
Cuts at: 0 150 4548
Size: 150 4398
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1010 4548
Size: 1010 3538
AlwNI CAG_nnn'CTG
Cuts at: 0 538 2089 4548
Size: 538 1551 2459
Fragments arranged by size:
2459 1551 538
ApaI G_GGCC'C
Cuts at: 0 1062 4548
Size: 1062 3486
ApoI r'AATT_y
Cuts at: 0 756 932 1704 1934 2161 2323 2695 2743
Size: 756 176 772 230 227 162 372 48
Cuts at: 2743 3184 3934 4548
Size: 441 750 614
Fragments arranged by size:
772 756 750 614 441 372 230 227
176 162 48
AseI AT'TA_AT
Cuts at: 0 59 560 1634 4548
Size: 59 501 1074 2914
Fragments arranged by size:
2914 1074 501 59
AvaI C'yCGr_G
Cuts at: 0 1391 2430 4548
Size: 1391 1039 2118
Fragments arranged by size:
2118 1391 1039
AvrII C'CTAG_G
Cuts at: 0 612 4548
Size: 612 3936
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2469 2502 3333 3366 4548
Size: 2469 33 831 33 1182
Fragments arranged by size:
2469 1182 831 33 33
BamHI G'GATC_C
Cuts at: 0 684 4047 4548
Size: 684 3363 501
Fragments arranged by size:
3363 684 501
BanI G'GyrC_C
Cuts at: 0 1743 2686 3895 4548
Size: 1743 943 1209 653
Fragments arranged by size:
1743 1209 943 653
BanII G_rGCy'C
Cuts at: 0 229 1062 1607 2242 4019 4548
Size: 229 833 545 635 1777 529
Fragments arranged by size:
1777 833 635 545 529 229
BbsI GAAGACnn'nnnn_
Cuts at: 0 1178 3274 3772 4548
Size: 1178 2096 498 776
Fragments arranged by size:
2096 1178 776 498
BbvCI CC'TCA_GC
Cuts at: 0 33 4548
Size: 33 4515
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 250 284 409 443 4548
Size: 250 34 125 34 4105
Fragments arranged by size:
4105 250 125 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 188 3793 4548
Size: 188 3605 755
Fragments arranged by size:
3605 755 188
BclI T'GATC_A
Cuts at: 0 1971 4548
Size: 1971 2577
BglI GCCn_nnn'nGGC
Cuts at: 0 1741 4548
Size: 1741 2807
BglII A'GATC_T
Cuts at: 0 1340 2918 4548
Size: 1340 1578 1630
Fragments arranged by size:
1630 1578 1340
Bme1580I G_kGCm'C
Cuts at: 0 1062 3898 4548
Size: 1062 2836 650
Fragments arranged by size:
2836 1062 650
BmrI ACTGGGnnnn_n'
Cuts at: 0 3770 4548
Size: 3770 778
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 1318 2495 2548 3860 3897 4548
Size: 587 731 1177 53 1312 37 651
Fragments arranged by size:
1312 1177 731 651 587 53 37
Bpu10I CC'TnA_GC
Cuts at: 0 33 438 476 3095 4548
Size: 33 405 38 2619 1453
Fragments arranged by size:
2619 1453 405 38 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2697 2991 4548
Size: 2697 294 1557
Fragments arranged by size:
2697 1557 294
BsaI GGTCTCn'nnnn_
Cuts at: 0 160 193 4548
Size: 160 33 4355
Fragments arranged by size:
4355 160 33
BsaBI GATnn'nnATC
Cuts at: 0 942 4548
Size: 942 3606
BsaWI w'CCGG_w
Cuts at: 0 150 3219 4548
Size: 150 3069 1329
Fragments arranged by size:
3069 1329 150
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1302 1332 1783 1813 4548
Size: 1302 30 451 30 2735
Fragments arranged by size:
2735 1302 451 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1404 1407 4548
Size: 1404 3 3141
Fragments arranged by size:
3141 1404 3
BseYI C'CCAG_C
Cuts at: 0 1015 2206 3541 4111 4548
Size: 1015 1191 1335 570 437
Fragments arranged by size:
1335 1191 1015 570 437
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 551 4009 4548
Size: 116 435 3458 539
Fragments arranged by size:
3458 539 435 116
BsiHKAI G_wGCw'C
Cuts at: 0 2046 2150 2588 4040 4306 4548
Size: 2046 104 438 1452 266 242
Fragments arranged by size:
2046 1452 438 266 242 104
BsmI GAATG_Cn'
Cuts at: 0 294 4548
Size: 294 4254
Bsp1286I G_dGCh'C
Cuts at: 0 229 1062 1607 2046 2150 2242 2588 3898
Size: 229 833 545 439 104 92 346 1310
Cuts at: 3898 4019 4040 4306 4548
Size: 121 21 266 242
Fragments arranged by size:
1310 833 545 439 346 266 242 229
121 104 92 21
BspHI T'CATG_A
Cuts at: 0 2328 3998 4548
Size: 2328 1670 550
Fragments arranged by size:
2328 1670 550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1039 1878 3354 4548
Size: 1039 839 1476 1194
Fragments arranged by size:
1476 1194 1039 839
BsrDI GCAATG_nn'
Cuts at: 0 107 311 3084 3965 4209 4548
Size: 107 204 2773 881 244 339
Fragments arranged by size:
2773 881 339 244 204 107
BsrFI r'CCGG_y
Cuts at: 0 150 4548
Size: 150 4398
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2341 2831 4548
Size: 2341 490 1717
Fragments arranged by size:
2341 1717 490
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3793 4548
Size: 3793 755
BstYI r'GATC_y
Cuts at: 0 471 684 1340 2918 4047 4548
Size: 471 213 656 1578 1129 501
Fragments arranged by size:
1578 1129 656 501 471 213
BtgI C'CryG_G
Cuts at: 0 2768 3726 4031 4041 4548
Size: 2768 958 305 10 507
Fragments arranged by size:
2768 958 507 305 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3068 4548
Size: 3068 1480
BtsI GCAGTG_nn'
Cuts at: 0 918 1080 1245 4548
Size: 918 162 165 3303
Fragments arranged by size:
3303 918 165 162
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2802 2837 4548
Size: 2802 35 1711
Fragments arranged by size:
2802 1711 35
DraI TTT'AAA
Cuts at: 0 1644 2072 2166 2276 3392 4548
Size: 1644 428 94 110 1116 1156
Fragments arranged by size:
1644 1156 1116 428 110 94
EaeI y'GGCC_r
Cuts at: 0 3723 4548
Size: 3723 825
EarI CTCTTCn'nnn_
Cuts at: 0 2382 3201 3576 4514 4548
Size: 2382 819 375 938 34
Fragments arranged by size:
2382 938 819 375 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3178 4548
Size: 3178 1370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 881 1084 1268 1318 2044 2481 2495
Size: 587 294 203 184 50 726 437 14
Cuts at: 2495 2548 2726 3860 3897 4548
Size: 53 178 1134 37 651
Fragments arranged by size:
1134 726 651 587 437 294 203 184
178 53 50 37 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 479 4548
Size: 479 4069
EcoO109I rG'GnC_Cy
Cuts at: 0 1058 1059 1095 2086 2841 3654 4151 4365
Size: 1058 1 36 991 755 813 497 214
Cuts at: 4365 4548
Size: 183
Fragments arranged by size:
1058 991 813 755 497 214 183 36
1
EcoRI G'AATT_C
Cuts at: 0 2695 4548
Size: 2695 1853
EcoRV GAT'ATC
Cuts at: 0 3063 4548
Size: 3063 1485
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 449 766 1239 1848 4548
Size: 449 317 473 609 2700
Fragments arranged by size:
2700 609 473 449 317
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 883 907 915 939 1474 1506 1701 1733
Size: 883 24 8 24 535 32 195 32
Cuts at: 1733 3807 3839 4252 4284 4397 4429 4548
Size: 2074 32 413 32 113 32 119
Fragments arranged by size:
2074 883 535 413 195 119 113 32
32 32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 0 849 1488 3470 4548
Size: 849 639 1982 1078
Fragments arranged by size:
1982 1078 849 639
HindIII A'AGCT_T
Cuts at: 0 743 1830 3027 4548
Size: 743 1087 1197 1521
Fragments arranged by size:
1521 1197 1087 743
MfeI C'AATT_G
Cuts at: 0 2225 4548
Size: 2225 2323
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 3191 3810 3986 4487 4548
Size: 409 2782 619 176 501 61
Fragments arranged by size:
2782 619 501 409 176 61
MslI CAynn'nnrTG
Cuts at: 0 1663 2072 4421 4548
Size: 1663 409 2349 127
Fragments arranged by size:
2349 1663 409 127
MspA1I CmG'CkG
Cuts at: 0 594 4548
Size: 594 3954
NcoI C'CATG_G
Cuts at: 0 2768 4031 4041 4548
Size: 2768 1263 10 507
Fragments arranged by size:
2768 1263 507 10
NdeI CA'TA_TG
Cuts at: 0 1347 3416 4548
Size: 1347 2069 1132
Fragments arranged by size:
2069 1347 1132
NspI r_CATG'y
Cuts at: 0 268 393 885 1662 2375 4245 4548
Size: 268 125 492 777 713 1870 303
Fragments arranged by size:
1870 777 713 492 303 268 125
PciI A'CATG_T
Cuts at: 0 881 4548
Size: 881 3667
PflMI CCAn_nnn'nTGG
Cuts at: 0 96 1240 4548
Size: 96 1144 3308
Fragments arranged by size:
3308 1144 96
PfoI T'CCnGG_A
Cuts at: 0 3155 4548
Size: 3155 1393
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3553 3585 4548
Size: 3553 32 963
Fragments arranged by size:
3553 963 32
PpuMI rG'GwC_Cy
Cuts at: 0 1095 2841 3654 4151 4548
Size: 1095 1746 813 497 397
Fragments arranged by size:
1746 1095 813 497 397
PshAI GACnn'nnGTC
Cuts at: 0 1376 4548
Size: 1376 3172
PsiI TTA'TAA
Cuts at: 0 4253 4548
Size: 4253 295
PspOMI G'GGCC_C
Cuts at: 0 1058 4548
Size: 1058 3490
PstI C_TGCA'G
Cuts at: 0 387 2447 2615 3349 4548
Size: 387 2060 168 734 1199
Fragments arranged by size:
2060 1199 734 387 168
PvuII CAG'CTG
Cuts at: 0 594 4548
Size: 594 3954
RsrII CG'GwC_CG
Cuts at: 0 4321 4548
Size: 4321 227
SanDI GG'GwC_CC
Cuts at: 0 4151 4548
Size: 4151 397
SapI GCTCTTCn'nnn_
Cuts at: 0 2382 3576 4548
Size: 2382 1194 972
Fragments arranged by size:
2382 1194 972
SbfI CC_TGCA'GG
Cuts at: 0 387 4548
Size: 387 4161
SexAI A'CCwGG_T
Cuts at: 0 1839 4548
Size: 1839 2709
SfcI C'TryA_G
Cuts at: 0 383 1374 2443 2611 2652 3345 4548
Size: 383 991 1069 168 41 693 1203
Fragments arranged by size:
1203 1069 991 693 383 168 41
SgrAI Cr'CCGG_yG
Cuts at: 0 150 4548
Size: 150 4398
SmaI CCC'GGG
Cuts at: 0 2432 4548
Size: 2432 2116
SmlI C'TyrA_G
Cuts at: 0 1261 2712 3006 3024 4548
Size: 1261 1451 294 18 1524
Fragments arranged by size:
1524 1451 1261 294 18
SpeI A'CTAG_T
Cuts at: 0 2482 4548
Size: 2482 2066
SspI AAT'ATT
Cuts at: 0 4106 4548
Size: 4106 442
StuI AGG'CCT
Cuts at: 0 323 2009 4548
Size: 323 1686 2539
Fragments arranged by size:
2539 1686 323
StyI C'CwwG_G
Cuts at: 0 458 612 2768 3851 4031 4041 4155 4361
Size: 458 154 2156 1083 180 10 114 206
Cuts at: 4361 4548
Size: 187
Fragments arranged by size:
2156 1083 458 206 187 180 154 114
10
SwaI ATTT'AAAT
Cuts at: 0 2072 4548
Size: 2072 2476
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1348 2029 2704 3555 4338 4548
Size: 1348 681 675 851 783 210
Fragments arranged by size:
1348 851 783 681 675 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1302 1334 3350 3382 4548
Size: 1302 32 2016 32 1166
Fragments arranged by size:
2016 1302 1166 32 32
XbaI T'CTAG_A
Cuts at: 0 120 4548
Size: 120 4428
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1429 3153 3376 3912 4548
Size: 1429 1724 223 536 636
Fragments arranged by size:
1724 1429 636 536 223
XmaI C'CCGG_G
Cuts at: 0 2430 4548
Size: 2430 2118
XmnI GAAnn'nnTTC
Cuts at: 0 947 1756 2288 4548
Size: 947 809 532 2260
Fragments arranged by size:
2260 947 809 532
Enzymes that do cut:
AarI AcuI AflII AflIII AgeI AlfI AlwNI ApaI
ApoI AseI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BsrFI BstAPI BstXI BstYI BtgI BtgZI BtsI
CspCI DraI EaeI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI Hin4I HincII HindIII MfeI MmeI
MslI MspA1I NcoI NdeI NspI PciI PflMI PfoI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuII RsrII
SanDI SapI SbfI SexAI SfcI SgrAI SmaI SmlI
SpeI SspI StuI StyI SwaI TaqII TstI XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AhdI AleI AloI
ApaLI AscI AsiSI BbeI BfrBI BlpI BmgBI BmtI
BplI BsaAI BsaHI BsiEI BsiWI BsmBI BspEI BsrBI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI
DraIII DrdI EagI EcoICRI FseI FspI FspAI HaeII
HpaI KasI KpnI MluI MscI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI PasI PmeI PmlI
PspXI PsrI PvuI SacI SacII SalI ScaI SfiI
SfoI SgfI SnaBI SphI SrfI TatI Tth111I XhoI
ZraI