Restriction map for pF1KA0984
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AflIII      AgeI        AlwI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspI        AvaI        AviII       AvrII       BamHI       
BbrPI       BbvCI       BclI        BlnI        BsaAI       
BsaBI       BsiWI       BsoBI       BspDI       BspHI       
BspLU11I    BsrBI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     ClaI        CpoI        DraIII      DrdI        
EagI        EclXI       Eco47III    Eco52I      EcoO65I     
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MaeII       MamI        MluI        MslI        MvnI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    RcaI        
RsrII       SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccIII       1569  TCCGGA               T^CCGGA
AcyI          638  GGCGCC               G[GA]^CG[CT]C
AflII        1327  CTTAAG               C^TTAAG
AhdI         1392  GACCAAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          552  CCTAAGG              CC^T[ACGT]AGG
AspEI        1392  GACCAAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI           99  TGGCCA               TGG^CCA
BanI          638  GGCGCC               G^G[CT][GA]CC
BbeI          638  GGCGCC               GGCGC^C
BciVI         384  GGATAC               GTATCC(6/5)
BfaI          658  CTAG                 C^TAG
BfrI         1327  CTTAAG               C^TTAAG
BglI         1529  GCCCTGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          109  GCTGAGC              GC^T[ACGT]AGC
BpmI         1202  CTCCAG               CTGGAG(16/14)
Bpu1102I      109  GCTGAGC              GC^T[ACGT]AGC
BsaHI         638  GGCGCC               G[GA]^CG[CT]C
BsaI          341  GAGACC               GGTCTC(1/5)
BsaWI        1569  TCCGGA               [AT]^CCGG[AT]
BseAI        1569  TCCGGA               T^CCGGA
BseRI         958  GAGGAG               GAGGAG(10/8)
BsgI          203  CTGCAC               GTGCAG(16/14)
BsiEI        1553  CGATCG               CG[GA][CT]^CG
BsmBI        1184  CGTCTC               CGTCTC(1/5)
BspEI        1569  TCCGGA               T^CCGGA
BspMI        1128  GCAGGT               ACCTGC(4/8)
BsrFI        1575  GCCGGT               [GA]^CCGG[CT]
Bsu36I        552  CCTAAGG              CC^T[ACGT]AGG
CelII         109  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       1575  GCCGGT               [GA]^CCGG[CT]
DraI          507  TTTAAA               TTT^AAA
DraII          65  AGGACCT              [GA]G^G[ACGT]CC[CT]
EaeI           99  TGGCCA               [CT]^GGCC[GA]
Eam1105I     1392  GACCAAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        552  CCTAAGG              CC^T[ACGT]AGG
EcoNI         642  CCTTACCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       65  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRV        1075  GATATC               GAT^ATC
FauI          135  GCGGG                CCCGC(4/6)
HaeII         638  GGCGCC               [GA]GCGC^[CT]
HgaI         1799  GCGTC                GACGC(5/10)
Hin1I         638  GGCGCC               G[GA]^CG[CT]C
HphI           72  TCACC                GGTGA(8/7)
KasI          638  GGCGCC               G^GCGCC
MaeI          658  CTAG                 C^TAG
MfeI          730  CAATTG               C^AATTG
MluNI          99  TGGCCA               TGG^CCA
MroI         1569  TCCGGA               T^CCGGA
MscI           99  TGGCCA               TGG^CCA
MunI          730  CAATTG               C^AATTG
NarI          638  GGCGCC               GG^CGCC
PpuMI          65  AGGACCT              [GA]G^G[AT]CC[CT]
PvuI         1553  CGATCG               CGAT^CG
SacI         1669  GAGCTC               GAGCT^C
ScaI         1097  AGTACT               AGT^ACT
SfoI          638  GGCGCC               GGC^GCC
SmlI         1327  CTTAAG               C^T[CT][GA]AG
TfiI          520  GATTC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI           50  GAATTC               [GA]^AATT[CT]
              619  GAATTC              
ApoI           50  GAATTC               [GA]^AATT[CT]
              619  GAATTC              
BcgI          855  CGATGAGGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1095  GCAGTACTCTCG        
BglII         512  AGATCT               A^GATCT
              973  AGATCT              
BsmFI        1415  GGGAC                GGGAC(10/14)
             1312  GTCCC               
Bsp1407I     1142  TGTACA               T^GTACA
             1498  TGTACA              
BsrDI         984  CATTGC               GCAATG(2/0)
              230  GCAATG              
BsrGI        1142  TGTACA               T^GTACA
             1498  TGTACA              
BsrI         1165  CCAGT                ACTGG(1/-1)
             1204  CCAGT               
BstYI         512  AGATCT               [GA]^GATC[CT]
              973  AGATCT              
CfoI            3  GCGC                 GCG^C
              639  GCGC                
EarI          389  CTCTTC               CTCTTC(1/4)
              838  GAAGAG              
EcoRI          50  GAATTC               G^AATTC
              619  GAATTC              
HhaI            3  GCGC                 GCG^C
              639  GCGC                
HinP1I          3  GCGC                 G^CGC
              639  GCGC                
Ksp632I       389  CTCTTC               CTCTTC(1/4)
              838  GAAGAG              
MaeIII       1168  GTGAC                ^GT[ACGT]AC
             1286  GTCAC               
MflI          512  AGATCT               [GA]^GATC[CT]
              973  AGATCT              
NspI          484  ACATGC               [GA]CATG^[CT]
             1382  ACATGC              
PvuII        1508  CAGCTG               CAG^CTG
             1658  CAGCTG              
SspBI        1142  TGTACA               T^GTACA
             1498  TGTACA              
Tsp45I       1168  GTGAC                ^GT[GC]AC
             1286  GTCAC               
XhoII         512  AGATCT               [GA]^GATC[CT]
              973  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AfaI        AluI        Alw44I      AlwNI       
ApaLI       AspHI       AvaII       BanII       BbsI        
BbvI        BcnI        BmyI        BpuAI       BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmI        
Bsp1286I    BssKI       BstF5I      BstNI       BstXI       
Cac8I       Cfr13I      DdeI        DpnII       DsaI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       ItaI        MboI        
MboII       MnlI        MseI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     
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