Restriction map for pF1KA0984
 (Linear) (Six-base) MAPSORT of: KIAA0984.seq  Check: 496  from: 1  to: 1941



KIAA0984.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     321     376    1108    1553    1884    1941 
   Size:        321      55     732     445     331      57

  Fragments arranged by size: 

                 732     445     331     321      57      55
 
AflII C'TTAA_G

Cuts at:      0    1327    1941 
   Size:       1327     614
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1397    1941 
   Size:       1397     544
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1564    1596    1941 
   Size:       1564      32     345

  Fragments arranged by size: 

                1564     345      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     381     805     969    1941 
   Size:        381     424     164     972

  Fragments arranged by size: 

                 972     424     381     164
 
ApaLI G'TGCA_C

Cuts at:      0    1678    1718    1873    1941 
   Size:       1678      40     155      68

  Fragments arranged by size: 

                1678     155      68      40
 
ApoI r'AATT_y

Cuts at:      0      50     619    1941 
   Size:         50     569    1322

  Fragments arranged by size: 

                1322     569      50
 
BanI G'GyrC_C

Cuts at:      0     638    1941 
   Size:        638    1303
 
BanII G_rGCy'C

Cuts at:      0     434    1632    1673    1941 
   Size:        434    1198      41     268

  Fragments arranged by size: 

                1198     434     268      41
 
BbeI G_GCGC'C

Cuts at:      0     642    1941 
   Size:        642    1299
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     327     441     519    1328    1941 
   Size:        327     114      78     809     613

  Fragments arranged by size: 

                 809     613     327     114      78
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     844     878    1084    1118    1941 
   Size:        844      34     206      34     823

  Fragments arranged by size: 

                 844     823     206      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     378    1941 
   Size:        378    1563
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1535    1941 
   Size:       1535     406
 
BglII A'GATC_T

Cuts at:      0     512     973    1941 
   Size:        512     461     968

  Fragments arranged by size: 

                 968     512     461
 
BlpI GC'TnA_GC

Cuts at:      0     110    1941 
   Size:        110    1831
 
Bme1580I G_kGCm'C

Cuts at:      0    1682    1722    1877    1941 
   Size:       1682      40     155      64

  Fragments arranged by size: 

                1682     155      64      40
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1187    1941 
   Size:       1187     754
 
Bpu10I CC'TnA_GC

Cuts at:      0    1672    1941 
   Size:       1672     269
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     335    1941 
   Size:        335    1606
 
BsaHI Gr'CG_yC

Cuts at:      0     639    1941 
   Size:        639    1302
 
BsaWI w'CCGG_w

Cuts at:      0    1569    1941 
   Size:       1569     372
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1564    1594    1941 
   Size:       1564      30     347

  Fragments arranged by size: 

                1564     347      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     973    1941 
   Size:        973     968
 
BseYI C'CCAG_C

Cuts at:      0     777    1467    1510    1795    1941 
   Size:        777     690      43     285     146

  Fragments arranged by size: 

                 777     690     285     146      43
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     188    1941 
   Size:        188    1753
 
BsiEI CG_ry'CG

Cuts at:      0    1556    1941 
   Size:       1556     385
 
BsiHKAI G_wGCw'C

Cuts at:      0    1673    1682    1722    1877    1941 
   Size:       1673       9      40     155      64

  Fragments arranged by size: 

                1673     155      64      40       9
 
BsmI GAATG_Cn'

Cuts at:      0    1175    1269    1483    1941 
   Size:       1175      94     214     458

  Fragments arranged by size: 

                1175     458     214      94
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1190    1941 
   Size:       1190     751
 
Bsp1286I G_dGCh'C

Cuts at:      0     434    1632    1673    1682    1722    1877    1941 
   Size:        434    1198      41       9      40     155      64

  Fragments arranged by size: 

                1198     434     155      64      41      40       9
 
BspEI T'CCGG_A

Cuts at:      0    1569    1941 
   Size:       1569     372
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1119    1941 
   Size:       1119     822
 
BsrDI GCAATG_nn'

Cuts at:      0     237     983    1941 
   Size:        237     746     958

  Fragments arranged by size: 

                 958     746     237
 
BsrFI r'CCGG_y

Cuts at:      0    1575    1941 
   Size:       1575     366
 
BsrGI T'GTAC_A

Cuts at:      0    1142    1498    1941 
   Size:       1142     356     443

  Fragments arranged by size: 

                1142     443     356
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1282    1467    1836    1941 
   Size:       1282     185     369     105

  Fragments arranged by size: 

                1282     369     185     105
 
BstYI r'GATC_y

Cuts at:      0     512     973    1941 
   Size:        512     461     968

  Fragments arranged by size: 

                 968     512     461
 
Bsu36I CC'TnA_GG

Cuts at:      0     553    1941 
   Size:        553    1388
 
BtgI C'CryG_G

Cuts at:      0      44     632     707    1348    1941 
   Size:         44     588      75     641     593

  Fragments arranged by size: 

                 641     593     588      75      44
 
BtsI GCAGTG_nn'

Cuts at:      0     466     884    1698    1941 
   Size:        466     418     814     243

  Fragments arranged by size: 

                 814     466     418     243
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     396     431    1941 
   Size:        396      35    1510

  Fragments arranged by size: 

                1510     396      35
 
DraI TTT'AAA

Cuts at:      0     509    1941 
   Size:        509    1432
 
EaeI y'GGCC_r

Cuts at:      0      99    1941 
   Size:         99    1842
 
EarI CTCTTCn'nnn_

Cuts at:      0     395     833    1941 
   Size:        395     438    1108

  Fragments arranged by size: 

                1108     438     395
 
EcoICRI GAG'CTC

Cuts at:      0    1671    1941 
   Size:       1671     270
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     321     376    1108    1187    1553    1884    1941 
   Size:        321      55     732      79     366     331      57

  Fragments arranged by size: 

                 732     366     331     321      79      57      55
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     646    1941 
   Size:        646    1295
 
EcoO109I rG'GnC_Cy

Cuts at:      0      66    1941 
   Size:         66    1875
 
EcoRI G'AATT_C

Cuts at:      0      50     619    1941 
   Size:         50     569    1322

  Fragments arranged by size: 

                1322     569      50
 
EcoRV GAT'ATC

Cuts at:      0    1077    1941 
   Size:       1077     864
 
HaeII r_GCGC'y

Cuts at:      0     642    1941 
   Size:        642    1299
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      25      57    1383    1415    1549    1581    1941 
   Size:         25      32    1326      32     134      32     360

  Fragments arranged by size: 

                1326     360     134      32      32      32      25
 
KasI G'GCGC_C

Cuts at:      0     638    1941 
   Size:        638    1303
 
MfeI C'AATT_G

Cuts at:      0     730    1941 
   Size:        730    1211
 
MscI TGG'CCA

Cuts at:      0     101    1941 
   Size:        101    1840
 
MspA1I CmG'CkG

Cuts at:      0    1510    1660    1695    1941 
   Size:       1510     150      35     246

  Fragments arranged by size: 

                1510     246     150      35
 
NarI GG'CG_CC

Cuts at:      0     639    1941 
   Size:        639    1302
 
NcoI C'CATG_G

Cuts at:      0      44     632     707    1941 
   Size:         44     588      75    1234

  Fragments arranged by size: 

                1234     588      75      44
 
NspI r_CATG'y

Cuts at:      0     488    1386    1941 
   Size:        488     898     555

  Fragments arranged by size: 

                 898     555     488
 
PfoI T'CCnGG_A

Cuts at:      0     999    1941 
   Size:        999     942
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1564    1596    1941 
   Size:       1564      32     345

  Fragments arranged by size: 

                1564     345      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      66    1941 
   Size:         66    1875
 
PsiI TTA'TAA

Cuts at:      0    1210    1941 
   Size:       1210     731
 
PstI C_TGCA'G

Cuts at:      0     926    1611    1659    1941 
   Size:        926     685      48     282

  Fragments arranged by size: 

                 926     685     282      48
 
PvuI CG_AT'CG

Cuts at:      0    1556    1941 
   Size:       1556     385
 
PvuII CAG'CTG

Cuts at:      0    1510    1660    1941 
   Size:       1510     150     281

  Fragments arranged by size: 

                1510     281     150
 
SacI G_AGCT'C

Cuts at:      0    1673    1941 
   Size:       1673     268
 
ScaI AGT'ACT

Cuts at:      0    1099    1941 
   Size:       1099     842
 
SfcI C'TryA_G

Cuts at:      0      84     624     922    1607    1655    1853    1941 
   Size:         84     540     298     685      48     198      88

  Fragments arranged by size: 

                 685     540     298     198      88      84      48
 
SfoI GGC'GCC

Cuts at:      0     640    1941 
   Size:        640    1301
 
SmlI C'TyrA_G

Cuts at:      0    1327    1941 
   Size:       1327     614
 
StyI C'CwwG_G

Cuts at:      0      44     183     632     707    1387    1755    1941 
   Size:         44     139     449      75     680     368     186

  Fragments arranged by size: 

                 680     449     368     186     139      75      44
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1697    1941 
   Size:       1697     244
 
TatI w'GTAC_w

Cuts at:      0    1097    1142    1498    1941 
   Size:       1097      45     356     443

  Fragments arranged by size: 

                1097     443     356      45
 

 Enzymes that do cut:

     AcuI    AflII     AhdI     AloI    AlwNI    ApaLI     ApoI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BglI    BglII     BlpI
 Bme1580I     BpmI   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspMI    BsrDI    BsrFI    BsrGI    BstXI    BstYI   Bsu36I     BtgI
     BtsI    CspCI     DraI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV    HaeII    Hin4I     KasI     MfeI     MscI
   MspA1I     NarI     NcoI     NspI     PfoI     PpiI    PpuMI     PsiI
     PstI     PvuI    PvuII     SacI     ScaI     SfcI     SfoI     SmlI
     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AleI     AlfI     ApaI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI    BbvCI     BclI    BfrBI    BmgBI     BmrI     BmtI
     BplI    BpuEI    BsaAI    BsaBI    BsiWI    BspHI    BsrBI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     ClaI   DraIII
     DrdI     EagI     EciI     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KpnI     MluI     MmeI     MslI     NaeI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PmeI     PmlI    PshAI   PspOMI    PspXI     PsrI    RsrII
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
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