Restriction map for pF1KA0460
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AflII       
AflIII      AhdI        Alw44I      ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AviII       
BbeI        BbrPI       BbvCI       BfrI        BsaBI       
BsaHI       BsiEI       BsiWI       Bsp1407I    BspDI       
BspLU11I    BsrDI       BsrGI       BssHII      BssSI       
BstAPI      ClaI        CpoI        EagI        Eam1105I    
EclXI       Eco52I      EcoRV       FseI        FspI        
Hin1I       HpaI        KasI        KpnI        KspI        
MamI        MluI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NspI        
PacI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SbfI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        XbaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AccII        3085  CGCG                 CG^CG
AccIII         86  TCCGGA               T^CCGGA
AgeI         2666  ACCGGT               A^CCGGT
AocI         3547  CCTAAGG              CC^T[ACGT]AGG
Aor51HI       491  AGCGCT               AGC^GCT
AspI         1476  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        4156  CCTAGG               C^CTAGG
BalI         2967  TGGCCA               TGG^CCA
BamHI         225  GGATCC               G^GATCC
BbsI          994  GAAGAC               GAAGAC(2/6)
BcgI         2030  GCACCTCAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1081  GTATCC               GTATCC(6/5)
BclI         3045  TGATCA               T^GATCA
BcnI         4139  CCCGG                CC^[GC]GG
BglII          91  AGATCT               A^GATCT
BlnI         4156  CCTAGG               C^CTAGG
BlpI         3898  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     3898  GCTGAGC              GC^T[ACGT]AGC
BpuAI         994  GAAGAC               GAAGAC(2/6)
BsaAI        2939  CACGTA               [CT]AC^GT[GA]
BseAI          86  TCCGGA               T^CCGGA
BsmI         1448  GCATTC               GAATGC(1/-1)
BspEI          86  TCCGGA               T^CCGGA
BspHI        1286  TCATGA               T^CATGA
BsrBI        2834  CCGCTC               CCGCTC(-3/-3)
BsrFI        2666  ACCGGT               [GA]^CCGG[CT]
Bst1107I      283  GTATAC               GTA^TAC
BstBI        3609  TTCGAA               TT^CGAA
BstUI        3085  CGCG                 CG^CG
BstZ17I       283  GTATAC               GTA^TAC
Bsu36I       3547  CCTAAGG              CC^T[ACGT]AGG
CelII        3898  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       2666  ACCGGT               [GA]^CCGG[CT]
DraIII       3832  CACGGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III      491  AGCGCT               AGC^GCT
Eco81I       3547  CCTAAGG              CC^T[ACGT]AGG
EcoRI        3680  GAATTC               G^AATTC
EcoT22I       758  ATGCAT               ATGCA^T
FauI         3083  CCCGC                CCCGC(4/6)
FbaI         3045  TGATCA               T^GATCA
MluNI        2967  TGGCCA               TGG^CCA
MroI           86  TCCGGA               T^CCGGA
MscI         2967  TGGCCA               TGG^CCA
MslI         3244  CATGGGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI         3085  CGCG                 CG^CG
NciI         4139  CCCGG                CC^[GC]GG
NsiI          758  ATGCAT               ATGCA^T
NspV         3609  TTCGAA               TT^CGAA
PaeR7I       3572  CTCGAG               C^TCGAG
PflFI        1476  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        2666  ACCGGT               A^CCGGT
PstI         1064  CTGCAG               CTGCA^G
RcaI         1286  TCATGA               T^CATGA
SacI         2230  GAGCTC               GAGCT^C
SalI         2901  GTCGAC               G^TCGAC
ScaI           55  AGTACT               AGT^ACT
SfuI         3609  TTCGAA               TT^CGAA
Tth111I      1476  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         3572  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI          283  GTATAC               GT^[AC][GT]AC
             2901  GTCGAC              
AvaI         2225  CCCGAG               C^[CT]CG[GA]G
             3572  CTCGAG              
BanI         2629  GGTGCC               G^G[CT][GA]CC
             3399  GGCACC              
BsaWI          86  TCCGGA               [AT]^CCGG[AT]
             2666  ACCGGT              
BsmBI        2602  CGTCTC               CGTCTC(1/5)
             3087  CGTCTC              
BsoBI        2225  CCCGAG               C^[CT]CG[GA]G
             3572  CTCGAG              
BstEII       2669  GGTCACC              G^GT[ACGT]ACC
             2845  GGTCACC             
BstPI        2669  GGTCACC              G^GT[ACGT]ACC
             2845  GGTCACC             
BstXI        1766  CCAACTCTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2529  CCAAGAATTTGG        
CfoI          492  GCGC                 GCG^C
             1982  GCGC                
DrdI         2258  GACCCTTTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3201  GACCGTAGAGTC        
EaeI         2967  TGGCCA               [CT]^GGCC[GA]
             4141  CGGCCA              
EcoNI        1321  CCTTCTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1419  CCTCTCCAAGG         
EcoO65I      2669  GGTCACC              G^GT[ACGT]ACC
             2845  GGTCACC             
HaeII         491  AGCGCT               [GA]GCGC^[CT]
             1981  AGCGCC              
HgaI          868  GACGC                GACGC(5/10)
             3086  GCGTC               
HhaI          492  GCGC                 GCG^C
             1982  GCGC                
HinP1I        492  GCGC                 G^CGC
             1982  GCGC                
HindIII       317  AAGCTT               A^AGCTT
             1653  AAGCTT              
MfeI          811  CAATTG               C^AATTG
             1718  CAATTG              
MunI          811  CAATTG               C^AATTG
             1718  CAATTG              
NcoI          938  CCATGG               C^CATGG
             4035  CCATGG              
SapI          472  GAAGAGC              GCTCTTC(1/4)
             3061  GAAGAGC             
SmlI          427  CTCAAG               C^T[CT][GA]AG
             3572  CTCGAG              
XcmI         1853  CCAAGCCAAAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2855  CCACGTCAGGCGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApaI        ApoI        Asp700I     AspHI       
AvaII       BanII       BbvI        BfaI        BglI        
BmyI        BpmI        BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EarI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspA1I      MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PpuMI       PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XhoII       XmnI        
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