Restriction map for pF1KA0460
 (Linear) (Six-base) MAPSORT of: KIAA0460.seq  Check: 9953  from: 1  to: 4272



KIAA0460.orf

 With 182 enzymes: SgfI * 

                        January 15, 2009 16:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     657    1383    1389    4272 
   Size:        657     726       6    2883

  Fragments arranged by size: 

                2883     726     657       6
 
AccI GT'mk_AC

Cuts at:      0     284    2902    4272 
   Size:        284    2618    1370

  Fragments arranged by size: 

                2618    1370     284
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     535     857    1501    1556    1685    1706    1826 
   Size:        224     311     322     644      55     129      21     120

Cuts at:   1826    1841    2619    4272 
   Size:         15     778    1653

  Fragments arranged by size: 

                1653     778     644     322     311     224     129     120
                  55      21      15
 
AfeI AGC'GCT

Cuts at:      0     493    4272 
   Size:        493    3779
 
AgeI A'CCGG_T

Cuts at:      0    2666    4272 
   Size:       2666    1606
 
AlwNI CAG_nnn'CTG

Cuts at:      0     835    1147    1489    3847    4272 
   Size:        835     312     342    2358     425

  Fragments arranged by size: 

                2358     835     425     342     312
 
ApaI G_GGCC'C

Cuts at:      0    2574    3138    3943    4030    4228    4272 
   Size:       2574     564     805      87     198      44

  Fragments arranged by size: 

                2574     805     564     198      87      44
 
ApoI r'AATT_y

Cuts at:      0     448     613     643     713    2533    2911    3680    4272 
   Size:        448     165      30      70    1820     378     769     592

  Fragments arranged by size: 

                1820     769     592     448     378     165      70      30
            
 
AvaI C'yCGr_G

Cuts at:      0    2225    3572    4272 
   Size:       2225    1347     700

  Fragments arranged by size: 

                2225    1347     700
 
AvrII C'CTAG_G

Cuts at:      0    4156    4272 
   Size:       4156     116
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1076    1109    4272 
   Size:       1076      33    3163

  Fragments arranged by size: 

                3163    1076      33
 
BamHI G'GATC_C

Cuts at:      0     225    4272 
   Size:        225    4047
 
BanI G'GyrC_C

Cuts at:      0    2629    3399    4272 
   Size:       2629     770     873

  Fragments arranged by size: 

                2629     873     770
 
BanII G_rGCy'C

Cuts at:      0    1739    2234    2529    2574    3138    3943    4030    4228 
   Size:       1739     495     295      45     564     805      87     198

Cuts at:   4228    4272 
   Size:         44

  Fragments arranged by size: 

                1739     805     564     495     295     198      87      45
                  44
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1001    4272 
   Size:       1001    3271
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2019    2053    4272 
   Size:       2019      34    2219

  Fragments arranged by size: 

                2219    2019      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1092    4272 
   Size:       1092    3180
 
BclI T'GATC_A

Cuts at:      0    3045    4272 
   Size:       3045    1227
 
BfrBI ATG'CAT

Cuts at:      0     760    4272 
   Size:        760    3512
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1711    3368    3519    4272 
   Size:       1711    1657     151     753

  Fragments arranged by size: 

                1711    1657     753     151
 
BglII A'GATC_T

Cuts at:      0      91    4272 
   Size:         91    4181
 
BlpI GC'TnA_GC

Cuts at:      0    3899    4272 
   Size:       3899     373
 
Bme1580I G_kGCm'C

Cuts at:      0    1635    2574    2989    3138    3402    3943    4030    4228 
   Size:       1635     939     415     149     264     541      87     198

Cuts at:   4228    4272 
   Size:         44

  Fragments arranged by size: 

                1635     939     541     415     264     198     149      87
                  44
 
BmgBI CAC'GTC

Cuts at:      0    2858    2900    4272 
   Size:       2858      42    1372

  Fragments arranged by size: 

                2858    1372      42
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     921    1135    1307    1531    1538    2753    2785    4272 
   Size:        921     214     172     224       7    1215      32    1487

  Fragments arranged by size: 

                1487    1215     921     224     214     172      32       7
            
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     645    1896    4009    4272 
   Size:        645    1251    2113     263

  Fragments arranged by size: 

                2113    1251     645     263
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1310    1611    1885    3686    3815    4272 
   Size:       1310     301     274    1801     129     457

  Fragments arranged by size: 

                1801    1310     457     301     274     129
 
Bpu10I CC'TnA_GC

Cuts at:      0    1267    1963    3973    4272 
   Size:       1267     696    2010     299

  Fragments arranged by size: 

                2010    1267     696     299
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412    4272 
   Size:        412    3860
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1854    2307    4172    4194    4272 
   Size:       1854     453    1865      22      78

  Fragments arranged by size: 

                1865    1854     453      78      22
 
BsaAI yAC'GTr

Cuts at:      0    2941    4272 
   Size:       2941    1331
 
BsaWI w'CCGG_w

Cuts at:      0      86    2666    4272 
   Size:         86    2580    1606

  Fragments arranged by size: 

                2580    1606      86
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     859     889    1149    1179    1858    1888    3684    3714 
   Size:        859      30     260      30     679      30    1796      30

Cuts at:   3714    4272 
   Size:        558

  Fragments arranged by size: 

                1796     859     679     558     260      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2774    3081    3084    3650    3692    3695    3761    4055 
   Size:       2774     307       3     566      42       3      66     294

Cuts at:   4055    4058    4272 
   Size:          3     214

  Fragments arranged by size: 

                2774     566     307     294     214      66      42       3
                   3       3
 
BseYI C'CCAG_C

Cuts at:      0    1209    1345    2179    2977    3028    3810    4272 
   Size:       1209     136     834     798      51     782     462

  Fragments arranged by size: 

                1209     834     798     782     462     136      51
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     573    1361    1442    1475    4272 
   Size:        573     788      81      33    2797

  Fragments arranged by size: 

                2797     788     573      81      33
 
BsiHKAI G_wGCw'C

Cuts at:      0    1570    2234    3068    3286    3674    3833    4272 
   Size:       1570     664     834     218     388     159     439

  Fragments arranged by size: 

                1570     834     664     439     388     218     159
 
BsmI GAATG_Cn'

Cuts at:      0    1448    4272 
   Size:       1448    2824
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2608    3093    4272 
   Size:       2608     485    1179

  Fragments arranged by size: 

                2608    1179     485
 
Bsp1286I G_dGCh'C

Cuts at:      0    1570    1635    1739    2234    2529    2574    2989    3068 
   Size:       1570      65     104     495     295      45     415      79

Cuts at:   3068    3138    3286    3402    3674    3833    3943    4030    4228 
   Size:         70     148     116     272     159     110      87     198

Cuts at:   4228    4272 
   Size:         44

  Fragments arranged by size: 

                1570     495     415     295     272     198     159     148
                 116     110     104      87      79      70      65      45
                  44
 
BspEI T'CCGG_A

Cuts at:      0      86    4272 
   Size:         86    4186
 
BspHI T'CATG_A

Cuts at:      0    1286    4272 
   Size:       1286    2986
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     657    1071    1383    1389    4272 
   Size:        657     414     312       6    2883

  Fragments arranged by size: 

                2883     657     414     312       6
 
BsrBI CCG'CTC

Cuts at:      0    2836    4272 
   Size:       2836    1436
 
BsrFI r'CCGG_y

Cuts at:      0    2666    4272 
   Size:       2666    1606
 
BstBI TT'CG_AA

Cuts at:      0    3610    4272 
   Size:       3610     662
 
BstEII G'GTnAC_C

Cuts at:      0    2669    2845    4272 
   Size:       2669     176    1427

  Fragments arranged by size: 

                2669    1427     176
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1773    2536    4272 
   Size:       1773     763    1736

  Fragments arranged by size: 

                1773    1736     763
 
BstYI r'GATC_y

Cuts at:      0      91     225     831    1242    3603    4272 
   Size:         91     134     606     411    2361     669

  Fragments arranged by size: 

                2361     669     606     411     134      91
 
BstZ17I GTA'TAC

Cuts at:      0     285    4272 
   Size:        285    3987
 
Bsu36I CC'TnA_GG

Cuts at:      0    3548    4272 
   Size:       3548     724
 
BtgI C'CryG_G

Cuts at:      0     938    2840    3933    4035    4272 
   Size:        938    1902    1093     102     237

  Fragments arranged by size: 

                1902    1093     938     237     102
 
BtsI GCAGTG_nn'

Cuts at:      0     391    1492    2130    2280    3738    4077    4272 
   Size:        391    1101     638     150    1458     339     195

  Fragments arranged by size: 

                1458    1101     638     391     339     195     150
 
DraI TTT'AAA

Cuts at:      0     261     794    1755    3375    4196    4272 
   Size:        261     533     961    1620     821      76

  Fragments arranged by size: 

                1620     961     821     533     261      76
 
DraIII CAC_nnn'GTG

Cuts at:      0    3837    4272 
   Size:       3837     435
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2264    3207    4272 
   Size:       2264     943    1065

  Fragments arranged by size: 

                2264    1065     943
 
EaeI y'GGCC_r

Cuts at:      0    2967    4141    4272 
   Size:       2967    1174     131

  Fragments arranged by size: 

                2967    1174     131
 
EarI CTCTTCn'nnn_

Cuts at:      0     467     614    1134    1178    1698    2340    3056    4272 
   Size:        467     147     520      44     520     642     716    1216

  Fragments arranged by size: 

                1216     716     642     520     520     467     147      44
            
 
EcoICRI GAG'CTC

Cuts at:      0    2232    4272 
   Size:       2232    2040
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     535     857    1310    1501    1556    1611    1685 
   Size:        224     311     322     453     191      55      55      74

Cuts at:   1685    1706    1826    1841    1885    2619    3686    3815    4272 
   Size:         21     120      15      44     734    1067     129     457

  Fragments arranged by size: 

                1067     734     457     453     322     311     224     191
                 129     120      74      55      55      44      21      15
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1325    1423    4272 
   Size:       1325      98    2849

  Fragments arranged by size: 

                2849    1325      98
 
EcoO109I rG'GnC_Cy

Cuts at:      0     462    2407    2570    2571    3134    3135    3890    3922 
   Size:        462    1945     163       1     563       1     755      32

Cuts at:   3922    3939    3940    3954    4087    4224    4225    4272 
   Size:         17       1      14     133     137       1      47

  Fragments arranged by size: 

                1945     755     563     462     163     137     133      47
                  32      17      14       1       1       1       1
 
EcoRI G'AATT_C

Cuts at:      0    3680    4272 
   Size:       3680     592
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1766    2568    4272 
   Size:       1766     802    1704

  Fragments arranged by size: 

                1766    1704     802
 
HaeII r_GCGC'y

Cuts at:      0     495    1985    4272 
   Size:        495    1490    2287

  Fragments arranged by size: 

                2287    1490     495
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     859     891     966     998    1012    1044    1149    1181 
   Size:        859      32      75      32      14      32     105      32

Cuts at:   1181    1380    1412    1858    1890    2122    2154    2302    2334 
   Size:        199      32     446      32     232      32     148      32

Cuts at:   2334    2590    2622    3319    3351    4091    4123    4272 
   Size:        256      32     697      32     740      32     149

  Fragments arranged by size: 

                 859     740     697     446     256     232     199     149
                 148     105      75      32      32      32      32      32
                  32      32      32      32      32      32      14
 
HincII GTy'rAC

Cuts at:      0     533     821    2903    3786    4272 
   Size:        533     288    2082     883     486

  Fragments arranged by size: 

                2082     883     533     486     288
 
HindIII A'AGCT_T

Cuts at:      0     317    1653    4272 
   Size:        317    1336    2619

  Fragments arranged by size: 

                2619    1336     317
 
MfeI C'AATT_G

Cuts at:      0     811    1718    4272 
   Size:        811     907    2554

  Fragments arranged by size: 

                2554     907     811
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     785    1790    2738    3461    4272 
   Size:        785    1005     948     723     811

  Fragments arranged by size: 

                1005     948     811     785     723
 
MscI TGG'CCA

Cuts at:      0    2969    4272 
   Size:       2969    1303
 
MslI CAynn'nnrTG

Cuts at:      0    3248    4272 
   Size:       3248    1024
 
MspA1I CmG'CkG

Cuts at:      0     211     351    1078    3356    4272 
   Size:        211     140     727    2278     916

  Fragments arranged by size: 

                2278     916     727     211     140
 
NcoI C'CATG_G

Cuts at:      0     938    4035    4272 
   Size:        938    3097     237

  Fragments arranged by size: 

                3097     938     237
 
NsiI A_TGCA'T

Cuts at:      0     762    4272 
   Size:        762    3510
 
PasI CC'CwG_GG

Cuts at:      0    1732    2203    2617    3926    4272 
   Size:       1732     471     414    1309     346

  Fragments arranged by size: 

                1732    1309     471     414     346
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2203    2998    4014    4036    4272 
   Size:       2203     795    1016      22     236

  Fragments arranged by size: 

                2203    1016     795     236      22
 
PfoI T'CCnGG_A

Cuts at:      0    1326    2576    3663    4272 
   Size:       1326    1250    1087     609

  Fragments arranged by size: 

                1326    1250    1087     609
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    4154    4186    4272 
   Size:       4154      32      86

  Fragments arranged by size: 

                4154      86      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    3890    3922    3954    4087    4272 
   Size:       3890      32      32     133     185

  Fragments arranged by size: 

                3890     185     133      32      32
 
PspOMI G'GGCC_C

Cuts at:      0    2570    3134    3939    4026    4224    4272 
   Size:       2570     564     805      87     198      48

  Fragments arranged by size: 

                2570     805     564     198      87      48
 
PstI C_TGCA'G

Cuts at:      0    1068    4272 
   Size:       1068    3204
 
PvuII CAG'CTG

Cuts at:      0     211     351    1078    4272 
   Size:        211     140     727    3194

  Fragments arranged by size: 

                3194     727     211     140
 
SacI G_AGCT'C

Cuts at:      0    2234    4272 
   Size:       2234    2038
 
SalI G'TCGA_C

Cuts at:      0    2901    4272 
   Size:       2901    1371
 
SanDI GG'GwC_CC

Cuts at:      0    3890    4087    4272 
   Size:       3890     197     185

  Fragments arranged by size: 

                3890     197     185
 
SapI GCTCTTCn'nnn_

Cuts at:      0     467    3056    4272 
   Size:        467    2589    1216

  Fragments arranged by size: 

                2589    1216     467
 
ScaI AGT'ACT

Cuts at:      0      57    4272 
   Size:         57    4215
 
SfcI C'TryA_G

Cuts at:      0     245    1064    3140    4272 
   Size:        245     819    2076    1132

  Fragments arranged by size: 

                2076    1132     819     245
 
SmlI C'TyrA_G

Cuts at:      0     427    3572    4272 
   Size:        427    3145     700

  Fragments arranged by size: 

                3145     700     427
 
StuI AGG'CCT

Cuts at:      0    1126    1502    1714    3362    4272 
   Size:       1126     376     212    1648     910

  Fragments arranged by size: 

                1648    1126     910     376     212
 
StyI C'CwwG_G

Cuts at:      0     938    1424    3149    3339    3482    3863    4035    4082 
   Size:        938     486    1725     190     143     381     172      47

Cuts at:   4082    4156    4272 
   Size:         74     116

  Fragments arranged by size: 

                1725     938     486     381     190     172     143     116
                  74      47
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2100    2104    2193    2804    2864    2868    3037    3597 
   Size:       2100       4      89     611      60       4     169     560

Cuts at:   3597    3961    4272 
   Size:        364     311

  Fragments arranged by size: 

                2100     611     560     364     311     169      89      60
                   4       4
 
TatI w'GTAC_w

Cuts at:      0      55     195    2185    4272 
   Size:         55     140    1990    2087

  Fragments arranged by size: 

                2087    1990     140      55
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3151    3183    4272 
   Size:       3151      32    1089

  Fragments arranged by size: 

                3151    1089      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1479    4272 
   Size:       1479    2793
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1860    2862    4272 
   Size:       1860    1002    1410

  Fragments arranged by size: 

                1860    1410    1002
 
XhoI C'TCGA_G

Cuts at:      0    3572    4272 
   Size:       3572     700
 
XmnI GAAnn'nnTTC

Cuts at:      0     601    3616    4166    4272 
   Size:        601    3015     550     106

  Fragments arranged by size: 

                3015     601     550     106
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI     AgeI    AlwNI     ApaI     ApoI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     DraI   DraIII     DrdI
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
    HaeII    Hin4I   HincII  HindIII     MfeI     MmeI     MscI     MslI
   MspA1I     NcoI     NsiI     PasI    PflMI     PfoI     PpiI    PpuMI
   PspOMI     PstI    PvuII     SacI     SalI    SanDI     SapI     ScaI
     SfcI     SmlI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII   AflIII     AhdI     AleI     AlfI
     AloI    ApaLI     AscI     AseI    AsiSI     BbeI    BbvCI     BmtI
    BsaBI    BsaHI    BsiEI    BsiWI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BtgZI     ClaI    CspCI     EagI     EciI    EcoRV     FseI
     FspI    FspAI     HpaI     KasI     KpnI     MluI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PciI
     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
    SacII     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     XbaI     XmaI
     ZraI
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