Restriction map for pF1KE0529
 (Linear) (Six-base) MAPSORT of: KIEE0529.seq  Check: 8712  from: 1  to: 642



KIEE0529.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

Acc65I G'GTAC_C

Cuts at:      0     140     506     642 
   Size:        140     366     136

  Fragments arranged by size: 

                 366     140     136
 
AflII C'TTAA_G

Cuts at:      0     607     642 
   Size:        607      35
 
AflIII A'CryG_T

Cuts at:      0       5     642 
   Size:          5     637
 
AlwNI CAG_nnn'CTG

Cuts at:      0     114     642 
   Size:        114     528
 
ApoI r'AATT_y

Cuts at:      0     241     519     642 
   Size:        241     278     123

  Fragments arranged by size: 

                 278     241     123
 
BanI G'GyrC_C

Cuts at:      0     140     506     642 
   Size:        140     366     136

  Fragments arranged by size: 

                 366     140     136
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     120     619     642 
   Size:        120     499      23

  Fragments arranged by size: 

                 499     120      23
 
BclI T'GATC_A

Cuts at:      0     410     642 
   Size:        410     232
 
BglII A'GATC_T

Cuts at:      0      65     642 
   Size:         65     577
 
Bme1580I G_kGCm'C

Cuts at:      0     480     642 
   Size:        480     162
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     295     642 
   Size:        295     347
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      90     304     367     443     569     642 
   Size:         90     214      63      76     126      73

  Fragments arranged by size: 

                 214     126      90      76      73      63
 
Bpu10I CC'TnA_GC

Cuts at:      0      28     642 
   Size:         28     614
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     427     642 
   Size:        427     215
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     151     181     642 
   Size:        151      30     461

  Fragments arranged by size: 

                 461     151      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     193     642 
   Size:        193     449
 
BseYI C'CCAG_C

Cuts at:      0      57     398     642 
   Size:         57     341     244

  Fragments arranged by size: 

                 341     244      57
 
Bsp1286I G_dGCh'C

Cuts at:      0     480     642 
   Size:        480     162
 
BspHI T'CATG_A

Cuts at:      0     407     642 
   Size:        407     235
 
BsrGI T'GTAC_A

Cuts at:      0     224     642 
   Size:        224     418
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     144     642 
   Size:        144     498
 
BstYI r'GATC_y

Cuts at:      0      65     110     350     642 
   Size:         65      45     240     292

  Fragments arranged by size: 

                 292     240      65      45
 
EaeI y'GGCC_r

Cuts at:      0      41     642 
   Size:         41     601
 
EarI CTCTTCn'nnn_

Cuts at:      0      97     409     642 
   Size:         97     312     233

  Fragments arranged by size: 

                 312     233      97
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     268     642 
   Size:        268     374
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      90     304     367     443     569     642 
   Size:         90     214      63      76     126      73

  Fragments arranged by size: 

                 214     126      90      76      73      63
 
KpnI G_GTAC'C

Cuts at:      0     144     510     642 
   Size:        144     366     132

  Fragments arranged by size: 

                 366     144     132
 
MscI TGG'CCA

Cuts at:      0      43     642 
   Size:         43     599
 
MslI CAynn'nnrTG

Cuts at:      0     184     642 
   Size:        184     458
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      39     307     642 
   Size:         39     268     335

  Fragments arranged by size: 

                 335     268      39
 
PshAI GACnn'nnGTC

Cuts at:      0     601     642 
   Size:        601      41
 
SapI GCTCTTCn'nnn_

Cuts at:      0      97     409     642 
   Size:         97     312     233

  Fragments arranged by size: 

                 312     233      97
 
SexAI A'CCwGG_T

Cuts at:      0     136     642 
   Size:        136     506
 
SfcI C'TryA_G

Cuts at:      0     330     642 
   Size:        330     312
 
SmlI C'TyrA_G

Cuts at:      0     607     642 
   Size:        607      35
 
TatI w'GTAC_w

Cuts at:      0     224     555     642 
   Size:        224     331      87

  Fragments arranged by size: 

                 331     224      87
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     141     642 
   Size:        141     501
 
XmnI GAAnn'nnTTC

Cuts at:      0       7     642 
   Size:          7     635
 

 Enzymes that do cut:

   Acc65I    AflII   AflIII    AlwNI     ApoI     BanI     BbsI     BclI
    BglII Bme1580I     BmrI     BpmI   Bpu10I     BsaI    BsaXI    BseRI
    BseYI Bsp1286I    BspHI    BsrGI    BstXI    BstYI     EaeI     EarI
     EciI  Eco57MI     KpnI     MscI     MslI    PflMI    PshAI     SapI
    SexAI     SfcI     SmlI     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI     BlpI    BmgBI     BmtI     BplI    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     MfeI     MluI
     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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