Restriction map for
pF1KB6487
(Circular) (Six-base) MAPSORT of: pF1KB6487.seq Check: 4135 from: 1 to: 4100
pF1KB6487 4100 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1138 1138
Size: 4100
Acc65I G'GTAC_C
Cuts at: 1116 1116
Size: 4100
AclI AA'CG_TT
Cuts at: 3940 3940
Size: 4100
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 1925 2357 3104 3372 653
Size: 1272 432 747 268 1381
Fragments arranged by size:
1381 1272 747 432 268
AflII C'TTAA_G
Cuts at: 685 685
Size: 4100
AflIII A'CryG_T
Cuts at: 1452 2556 3533 1452
Size: 1104 977 2019
Fragments arranged by size:
2019 1104 977
AgeI A'CCGG_T
Cuts at: 2488 2488
Size: 4100
AlwNI CAG_nnn'CTG
Cuts at: 492 594 1405 2972 492
Size: 102 811 1567 1620
Fragments arranged by size:
1620 1567 811 102
ApaI G_GGCC'C
Cuts at: 130 931 130
Size: 801 3299
ApaLI G'TGCA_C
Cuts at: 2870 2870
Size: 4100
ApoI r'AATT_y
Cuts at: 865 1104 3453 3709 865
Size: 239 2349 256 1256
Fragments arranged by size:
2349 1256 256 239
AseI AT'TA_AT
Cuts at: 20 20
Size: 4100
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4100
AvaI C'yCGr_G
Cuts at: 123 171 178 575 923 1120 123
Size: 48 7 397 348 197 3103
Fragments arranged by size:
3103 397 348 197 48 7
BamHI G'GATC_C
Cuts at: 1028 1125 1028
Size: 97 4003
BanI G'GyrC_C
Cuts at: 1040 1116 1530 1778 1813 1040
Size: 76 414 248 35 3327
Fragments arranged by size:
3327 414 248 76 35
BanII G_rGCy'C
Cuts at: 130 931 1114 2144 130
Size: 801 183 1030 2086
Fragments arranged by size:
2086 1030 801 183
BbeI G_GCGC'C
Cuts at: 1044 1534 1782 1044
Size: 490 248 3362
Fragments arranged by size:
3362 490 248
BbsI GAAGACnn'nnnn_
Cuts at: 1077 1077
Size: 4100
BciVI GTATCCnnnnn_n'
Cuts at: 1998 2759 4100 1998
Size: 761 1341 1998
Fragments arranged by size:
1998 1341 761
BclI T'GATC_A
Cuts at: 1620 1620
Size: 4100
BglI GCCn_nnn'nGGC
Cuts at: 112 142 112
Size: 30 4070
BglII A'GATC_T
Cuts at: 767 1615 3652 767
Size: 848 2037 1215
Fragments arranged by size:
2037 1215 848
BlpI GC'TnA_GC
Cuts at: 1212 1212
Size: 4100
Bme1580I G_kGCm'C
Cuts at: 130 931 1725 1818 2874 130
Size: 801 794 93 1056 1356
Fragments arranged by size:
1356 1056 801 794 93
BmrI ACTGGGnnnn_n'
Cuts at: 457 1312 1393 1728 3872 457
Size: 855 81 335 2144 685
Fragments arranged by size:
2144 855 685 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 4100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 967 989 967
Size: 22 4078
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 730 1276 2155 2647 2945 190
Size: 540 546 879 492 298 1345
Fragments arranged by size:
1345 879 546 540 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 181 208 886 3804 181
Size: 27 678 2918 477
Fragments arranged by size:
2918 678 477 27
BsaAI yAC'GTr
Cuts at: 234 1453 234
Size: 1219 2881
BsaBI GATnn'nnATC
Cuts at: 6 1614 3651 6
Size: 1608 2037 455
Fragments arranged by size:
2037 1608 455
BsaHI Gr'CG_yC
Cuts at: 1041 1531 1779 2481 3977 1041
Size: 490 248 702 1496 1164
Fragments arranged by size:
1496 1164 702 490 248
BsaWI w'CCGG_w
Cuts at: 368 1289 1810 2488 2762 2909 3320 368
Size: 921 521 678 274 147 411 1148
Fragments arranged by size:
1148 921 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 341 363 371 393 523 553 637 667 341
Size: 22 8 22 130 30 84 30 3774
Fragments arranged by size:
3774 130 84 30 30 22 22 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 595 904 595
Size: 309 3791
BseYI C'CCAG_C
Cuts at: 459 936 1525 2283 2860 3356 459
Size: 477 589 758 577 496 1203
Fragments arranged by size:
1203 758 589 577 496 477
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 613 804 505
Size: 108 191 3801
Fragments arranged by size:
3801 191 108
BsiEI CG_ry'CG
Cuts at: 104 324 378 1642 1688 2896 104
Size: 220 54 1264 46 1208 1308
Fragments arranged by size:
1308 1264 1208 220 54 46
BsiHKAI G_wGCw'C
Cuts at: 1016 1114 1892 2082 2874 1016
Size: 98 778 190 792 2242
Fragments arranged by size:
2242 792 778 190 98
Bsp1286I G_dGCh'C
Cuts at: 130 931 1016 1114 1725 1818 1892 2082 2144
Size: 801 85 98 611 93 74 190 62
Cuts at: 2144 2874 130
Size: 730 1356
Fragments arranged by size:
1356 801 730 611 190 98 93 85
74 62
BspEI T'CCGG_A
Cuts at: 3320 3320
Size: 4100
BspHI T'CATG_A
Cuts at: 3276 3276
Size: 4100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 413 1150 1364 1666 2047 413
Size: 737 214 302 381 2466
Fragments arranged by size:
2466 737 381 302 214
BsrBI CCG'CTC
Cuts at: 50 374 2392 2446 3930 4095 50
Size: 324 2018 54 1484 165 55
Fragments arranged by size:
2018 1484 324 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2012 2012
Size: 4100
BsrFI r'CCGG_y
Cuts at: 2098 2279 2488 2098
Size: 181 209 3710
Fragments arranged by size:
3710 209 181
BssSI C'ACGA_G
Cuts at: 2371 2729 2371
Size: 358 3742
BstAPI GCAn_nnn'nTGC
Cuts at: 1477 1477
Size: 4100
BstBI TT'CG_AA
Cuts at: 2461 2461
Size: 4100
BstXI CCAn_nnnn'nTGG
Cuts at: 615 615
Size: 4100
BstYI r'GATC_y
Cuts at: 1 767 881 1028 1125 1592 1615 1950 2196
Size: 766 114 147 97 467 23 335 246
Cuts at: 2196 3208 3294 3306 3652 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 766 467 449 346 335 246 147
114 97 86 23 12
BtgI C'CryG_G
Cuts at: 140 560 614 726 987 140
Size: 420 54 112 261 3253
Fragments arranged by size:
3253 420 261 112 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2050 2231 2394 2050
Size: 181 163 3756
Fragments arranged by size:
3756 181 163
BtsI GCAGTG_nn'
Cuts at: 424 1343 424
Size: 919 3181
DraI TTT'AAA
Cuts at: 1099 1099
Size: 4100
DrdI GACnn_nn'nnGTC
Cuts at: 1806 2664 1806
Size: 858 3242
EaeI y'GGCC_r
Cuts at: 321 375 596 984 1071 1685 1859 2250 2277
Size: 54 221 388 87 614 174 391 27
Cuts at: 2277 3622 321
Size: 1345 799
Fragments arranged by size:
1345 799 614 391 388 221 174 87
54 27
EagI C'GGCC_G
Cuts at: 321 375 1685 321
Size: 54 1310 2736
Fragments arranged by size:
2736 1310 54
EarI CTCTTCn'nnn_
Cuts at: 745 995 2123 2333 745
Size: 250 1128 210 2512
Fragments arranged by size:
2512 1128 250 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 130 2308 2618 2764 3910 130
Size: 2178 310 146 1146 320
Fragments arranged by size:
2178 1146 320 310 146
EcoICRI GAG'CTC
Cuts at: 1112 1112
Size: 4100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 967 989 1925 2357 3104 3372 653
Size: 314 22 936 432 747 268 1381
Fragments arranged by size:
1381 936 747 432 314 268 22
EcoNI CCTnn'n_nnAGG
Cuts at: 484 496 946 484
Size: 12 450 3638
Fragments arranged by size:
3638 450 12
EcoO109I rG'GnC_Cy
Cuts at: 489 927 1239 489
Size: 438 312 3350
Fragments arranged by size:
3350 438 312
EcoRI G'AATT_C
Cuts at: 1104 1104
Size: 4100
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 4100
FspI TGC'GCA
Cuts at: 277 1881 277
Size: 1604 2496
HaeII r_GCGC'y
Cuts at: 517 1044 1534 1782 2804 3783 517
Size: 527 490 248 1022 979 834
Fragments arranged by size:
1022 979 834 527 490 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 363 395 523 555 1942 1972 1974 2004 363
Size: 32 128 32 1387 30 2 30 2459
Fragments arranged by size:
2459 1387 128 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1139 1139
Size: 4100
KasI G'GCGC_C
Cuts at: 1040 1530 1778 1040
Size: 490 248 3362
Fragments arranged by size:
3362 490 248
KpnI G_GTAC'C
Cuts at: 1120 1120
Size: 4100
MluI A'CGCG_T
Cuts at: 3533 3533
Size: 4100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2771 2955 2771
Size: 184 3916
MscI TGG'CCA
Cuts at: 598 1073 1861 598
Size: 475 788 2837
Fragments arranged by size:
2837 788 475
MslI CAynn'nnrTG
Cuts at: 559 2089 2216 559
Size: 1530 127 2443
Fragments arranged by size:
2443 1530 127
MspA1I CmG'CkG
Cuts at: 142 213 320 510 546 967 989 1211 1525
Size: 71 107 190 36 421 22 222 314
Cuts at: 1525 1885 2898 3143 142
Size: 360 1013 245 1099
Fragments arranged by size:
1099 1013 421 360 314 245 222 190
107 71 36 22
NaeI GCC'GGC
Cuts at: 2281 2281
Size: 4100
NarI GG'CG_CC
Cuts at: 1041 1531 1779 1041
Size: 490 248 3362
Fragments arranged by size:
3362 490 248
NcoI C'CATG_G
Cuts at: 560 560
Size: 4100
NgoMIV G'CCGG_C
Cuts at: 2279 2279
Size: 4100
NotI GC'GGCC_GC
Cuts at: 321 321
Size: 4100
NspI r_CATG'y
Cuts at: 1153 1653 2560 1153
Size: 500 907 2693
Fragments arranged by size:
2693 907 500
PciI A'CATG_T
Cuts at: 2556 2556
Size: 4100
PfoI T'CCnGG_A
Cuts at: 944 944
Size: 4100
PmeI GTTT'AAAC
Cuts at: 1099 1099
Size: 4100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3239 3271 3239
Size: 32 4068
PshAI GACnn'nnGTC
Cuts at: 1061 1061
Size: 4100
PspOMI G'GGCC_C
Cuts at: 126 927 126
Size: 801 3299
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 214 246 214
Size: 32 4068
PstI C_TGCA'G
Cuts at: 521 1147 1832 521
Size: 626 685 2789
Fragments arranged by size:
2789 685 626
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4100
PvuII CAG'CTG
Cuts at: 510 967 1525 1885 510
Size: 457 558 360 2725
Fragments arranged by size:
2725 558 457 360
RsrII CG'GwC_CG
Cuts at: 2295 2295
Size: 4100
SacI G_AGCT'C
Cuts at: 1114 1114
Size: 4100
SacII CC_GC'GG
Cuts at: 143 990 143
Size: 847 3253
SalI G'TCGA_C
Cuts at: 1137 1137
Size: 4100
SapI GCTCTTCn'nnn_
Cuts at: 2123 2333 2123
Size: 210 3890
SbfI CC_TGCA'GG
Cuts at: 1147 1147
Size: 4100
ScaI AGT'ACT
Cuts at: 652 3617 652
Size: 2965 1135
SexAI A'CCwGG_T
Cuts at: 479 479
Size: 4100
SfcI C'TryA_G
Cuts at: 33 517 1143 1828 2821 3012 33
Size: 484 626 685 993 191 1121
Fragments arranged by size:
1121 993 685 626 484 191
SfoI GGC'GCC
Cuts at: 1042 1532 1780 1042
Size: 490 248 3362
Fragments arranged by size:
3362 490 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4100
SmaI CCC'GGG
Cuts at: 577 925 1122 577
Size: 348 197 3555
Fragments arranged by size:
3555 348 197
SmlI C'TyrA_G
Cuts at: 178 205 685 745 1255 2170 2662 2924 178
Size: 27 480 60 510 915 492 262 1354
Fragments arranged by size:
1354 915 510 492 480 262 60 27
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4100
SphI G_CATG'C
Cuts at: 1153 1653 1153
Size: 500 3600
StuI AGG'CCT
Cuts at: 121 121
Size: 4100
StyI C'CwwG_G
Cuts at: 560 857 1234 1588 560
Size: 297 377 354 3072
Fragments arranged by size:
3072 377 354 297
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 345 355 1066 1683 1816 2275 2484 345
Size: 10 711 617 133 459 209 1961
Fragments arranged by size:
1961 711 617 459 209 133 10
TatI w'GTAC_w
Cuts at: 650 3615 650
Size: 2965 1135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 341 373 3617 3649 341
Size: 32 3244 32 792
Fragments arranged by size:
3244 792 32 32
Tth111I GACn'n_nGTC
Cuts at: 537 1897 537
Size: 1360 2740
XbaI T'CTAG_A
Cuts at: 1131 1131
Size: 4100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1595 1595
Size: 4100
XhoI C'TCGA_G
Cuts at: 178 178
Size: 4100
XmaI C'CCGG_G
Cuts at: 575 923 1120 575
Size: 348 197 3555
Fragments arranged by size:
3555 348 197
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PciI PfoI PmeI PpiI
PshAI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NruI NsiI PacI PasI PflMI
PmlI PpuMI PsiI PspXI SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XmnI ZraI