Restriction map for
pF1KB6487
(Linear) (Six-base) MAPSORT of: KIBB6487.seq Check: 9343 from: 1 to: 987
KIBB6487.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 987
Size: 545 442
AflII C'TTAA_G
Cuts at: 0 577 987
Size: 577 410
AlwNI CAG_nnn'CTG
Cuts at: 0 384 486 987
Size: 384 102 501
Fragments arranged by size:
501 384 102
ApaI G_GGCC'C
Cuts at: 0 22 823 987
Size: 22 801 164
Fragments arranged by size:
801 164 22
ApoI r'AATT_y
Cuts at: 0 757 987
Size: 757 230
AvaI C'yCGr_G
Cuts at: 0 15 63 70 467 815 987
Size: 15 48 7 397 348 172
Fragments arranged by size:
397 348 172 48 15 7
BamHI G'GATC_C
Cuts at: 0 920 987
Size: 920 67
BanI G'GyrC_C
Cuts at: 0 932 987
Size: 932 55
BanII G_rGCy'C
Cuts at: 0 22 823 987
Size: 22 801 164
Fragments arranged by size:
801 164 22
BbeI G_GCGC'C
Cuts at: 0 936 987
Size: 936 51
BbsI GAAGACnn'nnnn_
Cuts at: 0 969 987
Size: 969 18
BglI GCCn_nnn'nGGC
Cuts at: 0 34 987
Size: 34 953
BglII A'GATC_T
Cuts at: 0 659 987
Size: 659 328
Bme1580I G_kGCm'C
Cuts at: 0 22 823 987
Size: 22 801 164
Fragments arranged by size:
801 164 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 349 987
Size: 349 638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 859 881 987
Size: 859 22 106
Fragments arranged by size:
859 106 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 622 987
Size: 82 540 365
Fragments arranged by size:
540 365 82
BsaI GGTCTCn'nnnn_
Cuts at: 0 73 100 778 987
Size: 73 27 678 209
Fragments arranged by size:
678 209 73 27
BsaAI yAC'GTr
Cuts at: 0 126 987
Size: 126 861
BsaHI Gr'CG_yC
Cuts at: 0 933 987
Size: 933 54
BsaWI w'CCGG_w
Cuts at: 0 260 987
Size: 260 727
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 233 255 263 285 415 445 529 559
Size: 233 22 8 22 130 30 84 30
Cuts at: 559 987
Size: 428
Fragments arranged by size:
428 233 130 84 30 30 22 22
8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 487 796 987
Size: 487 309 191
Fragments arranged by size:
487 309 191
BseYI C'CCAG_C
Cuts at: 0 351 828 987
Size: 351 477 159
Fragments arranged by size:
477 351 159
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 505 696 987
Size: 397 108 191 291
Fragments arranged by size:
397 291 191 108
BsiEI CG_ry'CG
Cuts at: 0 216 270 987
Size: 216 54 717
Fragments arranged by size:
717 216 54
BsiHKAI G_wGCw'C
Cuts at: 0 908 987
Size: 908 79
Bsp1286I G_dGCh'C
Cuts at: 0 22 823 908 987
Size: 22 801 85 79
Fragments arranged by size:
801 85 79 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 305 987
Size: 305 682
BsrBI CCG'CTC
Cuts at: 0 266 987
Size: 266 721
BstXI CCAn_nnnn'nTGG
Cuts at: 0 507 987
Size: 507 480
BstYI r'GATC_y
Cuts at: 0 659 773 920 987
Size: 659 114 147 67
Fragments arranged by size:
659 147 114 67
BtgI C'CryG_G
Cuts at: 0 32 452 506 618 879 987
Size: 32 420 54 112 261 108
Fragments arranged by size:
420 261 112 108 54 32
BtsI GCAGTG_nn'
Cuts at: 0 316 987
Size: 316 671
EaeI y'GGCC_r
Cuts at: 0 213 267 488 876 963 987
Size: 213 54 221 388 87 24
Fragments arranged by size:
388 221 213 87 54 24
EagI C'GGCC_G
Cuts at: 0 213 267 987
Size: 213 54 720
Fragments arranged by size:
720 213 54
EarI CTCTTCn'nnn_
Cuts at: 0 637 887 987
Size: 637 250 100
Fragments arranged by size:
637 250 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 987
Size: 22 965
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 859 881 987
Size: 545 314 22 106
Fragments arranged by size:
545 314 106 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 376 388 838 987
Size: 376 12 450 149
Fragments arranged by size:
450 376 149 12
EcoO109I rG'GnC_Cy
Cuts at: 0 381 819 987
Size: 381 438 168
Fragments arranged by size:
438 381 168
FspI TGC'GCA
Cuts at: 0 169 987
Size: 169 818
HaeII r_GCGC'y
Cuts at: 0 409 936 987
Size: 409 527 51
Fragments arranged by size:
527 409 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 255 287 415 447 987
Size: 255 32 128 32 540
Fragments arranged by size:
540 255 128 32 32
KasI G'GCGC_C
Cuts at: 0 932 987
Size: 932 55
MscI TGG'CCA
Cuts at: 0 490 965 987
Size: 490 475 22
Fragments arranged by size:
490 475 22
MslI CAynn'nnrTG
Cuts at: 0 451 987
Size: 451 536
MspA1I CmG'CkG
Cuts at: 0 34 105 212 402 438 859 881 987
Size: 34 71 107 190 36 421 22 106
Fragments arranged by size:
421 190 107 106 71 36 34 22
NarI GG'CG_CC
Cuts at: 0 933 987
Size: 933 54
NcoI C'CATG_G
Cuts at: 0 452 987
Size: 452 535
NotI GC'GGCC_GC
Cuts at: 0 213 987
Size: 213 774
PfoI T'CCnGG_A
Cuts at: 0 836 987
Size: 836 151
PshAI GACnn'nnGTC
Cuts at: 0 953 987
Size: 953 34
PspOMI G'GGCC_C
Cuts at: 0 18 819 987
Size: 18 801 168
Fragments arranged by size:
801 168 18
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 106 138 987
Size: 106 32 849
Fragments arranged by size:
849 106 32
PstI C_TGCA'G
Cuts at: 0 413 987
Size: 413 574
PvuII CAG'CTG
Cuts at: 0 402 859 987
Size: 402 457 128
Fragments arranged by size:
457 402 128
SacII CC_GC'GG
Cuts at: 0 35 882 987
Size: 35 847 105
Fragments arranged by size:
847 105 35
ScaI AGT'ACT
Cuts at: 0 544 987
Size: 544 443
SexAI A'CCwGG_T
Cuts at: 0 371 987
Size: 371 616
SfcI C'TryA_G
Cuts at: 0 409 987
Size: 409 578
SfoI GGC'GCC
Cuts at: 0 934 987
Size: 934 53
SmaI CCC'GGG
Cuts at: 0 469 817 987
Size: 469 348 170
Fragments arranged by size:
469 348 170
SmlI C'TyrA_G
Cuts at: 0 70 97 577 637 987
Size: 70 27 480 60 350
Fragments arranged by size:
480 350 70 60 27
StuI AGG'CCT
Cuts at: 0 13 987
Size: 13 974
StyI C'CwwG_G
Cuts at: 0 452 749 987
Size: 452 297 238
Fragments arranged by size:
452 297 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 237 247 958 987
Size: 237 10 711 29
Fragments arranged by size:
711 237 29 10
TatI w'GTAC_w
Cuts at: 0 542 987
Size: 542 445
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 233 265 987
Size: 233 32 722
Fragments arranged by size:
722 233 32
Tth111I GACn'n_nGTC
Cuts at: 0 429 987
Size: 429 558
XhoI C'TCGA_G
Cuts at: 0 70 987
Size: 70 917
XmaI C'CCGG_G
Cuts at: 0 467 815 987
Size: 467 348 172
Fragments arranged by size:
467 348 172
Enzymes that do cut:
AcuI AflII AlwNI ApaI ApoI AvaI BamHI BanI
BanII BbeI BbsI BglI BglII Bme1580I BmrI BpmI
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BstXI BstYI
BtgI BtsI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I KasI MscI MslI MspA1I
NarI NcoI NotI PfoI PshAI PspOMI PsrI PstI
PvuII SacII ScaI SexAI SfcI SfoI SmaI SmlI
StuI StyI TaqII TatI TstI Tth111I XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BlpI
BmgBI BmtI BplI Bpu10I BsaBI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EcoICRI EcoRI EcoRV FalI FseI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI NaeI
NdeI NgoMIV NheI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PsiI PspXI
PvuI RsrII SacI SalI SanDI SapI SbfI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
XbaI XcmI XmnI ZraI