Restriction map for pF1KB6319
##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvaII       AviII       AvrII       BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BseAI       BsiEI       BsiHKAI     
BsiWI       BsmFI       BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HaeII       Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MflI        MluI        MroI        MslI        MspA1I      
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    StuI        SwaI        TspRI       Tth111I     
Van91I      XcmI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccII         151  CGCG                 CG^CG
AcsI          400  GAATTT               [GA]^AATT[CT]
AfaI          685  GTAC                 GT^AC
AlwNI         491  CAGGTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          189  GGGCCC               GGGCC^C
ApoI          400  GAATTT               [GA]^AATT[CT]
Asp700I        40  GAACTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI           26  CCCGAG               C^[CT]CG[GA]G
BalI          579  TGGCCA               TGG^CCA
BanI          169  GGCACC               G^G[CT][GA]CC
BanII         189  GGGCCC               G[GA]GC[CT]^C
BclI          648  TGATCA               T^GATCA
BmyI          189  GGGCCC               G[AGT]GC[ACT]^C
BsaWI          59  ACCGGA               [AT]^CCGG[AT]
BseRI         123  CTCCTC               GAGGAG(10/8)
BsgI          310  GTGCAG               GTGCAG(16/14)
BsmBI          94  GAGACG               CGTCTC(1/5)
BsmI          540  GAATGC               GAATGC(1/-1)
BsoBI          26  CCCGAG               C^[CT]CG[GA]G
Bsp1286I      189  GGGCCC               G[AGT]GC[ACT]^C
Bsp1407I      684  TGTACA               T^GTACA
BspMI         204  GCAGGT               ACCTGC(4/8)
BsrGI         684  TGTACA               T^GTACA
BstAPI        303  GCAGGGCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        659  CATCC                GGATG(2/0)
BstUI         151  CGCG                 CG^CG
DpnII         649  GATC                 ^GATC
DraII         189  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EaeI          579  TGGCCA               [CT]^GGCC[GA]
EarI          762  GAAGAG               CTCTTC(1/4)
EcoO109I      189  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       230  CCAAGG               C^C[AT][AT]GG
FbaI          648  TGATCA               T^GATCA
FokI          659  CATCC                GGATG(9/13)
HgaI           96  GACGC                GACGC(5/10)
HphI          651  TCACC                GGTGA(8/7)
Ksp632I       762  GAAGAG               CTCTTC(1/4)
MboI          649  GATC                 ^GATC
MluNI         579  TGGCCA               TGG^CCA
MscI          579  TGGCCA               TGG^CCA
MseI          679  TTAA                 T^TAA
MvnI          151  CGCG                 CG^CG
NdeII         649  GATC                 ^GATC
NspI          714  ACATGC               [GA]CATG^[CT]
PleI          665  GACTC                GAGTC(4/5)
RsaI          685  GTAC                 GT^AC
Sau3AI        649  GATC                 ^GATC
SspBI         684  TGTACA               T^GTACA
SspI          418  AATATT               AAT^ATT
StyI          230  CCAAGG               C^C[AT][AT]GG
TaqI          510  TCGA                 T^CGA
TfiI          504  GAATC                G^A[AT]TC
Tru9I         679  TTAA                 T^TAA
Tsp45I        331  GTGAC                ^GT[GC]AC
Tsp509I       401  AATT                 ^AATT
TthHB8I       510  TCGA                 T^CGA
XbaI          273  TCTAGA               T^CTAGA
XmnI           40  GAACTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AocI           83  CCTCAGG              CC^T[ACGT]AGG
              587  CCTTAGG             
BfaI          274  CTAG                 C^TAG
              445  CTAG                
BpmI          295  CTCCAG               CTGGAG(16/14)
              488  CTCCAG              
BsmAI          94  GAGAC                GTCTC(1/5)
                3  GTCTC               
Bsu36I         83  CCTCAGG              CC^T[ACGT]AGG
              587  CCTTAGG             
CfoI          202  GCGC                 GCG^C
              549  GCGC                
Cfr13I        189  GGGCC                G^G[ACGT]CC
              190  GGCCC               
Eco81I         83  CCTCAGG              CC^T[ACGT]AGG
              587  CCTTAGG             
FauI          149  CCCGC                CCCGC(4/6)
              187  GCGGG               
HhaI          202  GCGC                 GCG^C
              549  GCGC                
HinP1I        202  GCGC                 G^CGC
              549  GCGC                
HinfI         504  GAATC                G^A[ACGT]TC
              665  GACTC               
MaeI          274  CTAG                 C^TAG
              445  CTAG                
NlaIII        369  CATG                 CATG^
              715  CATG                
Sau96I        189  GGGCC                G^G[ACGT]CC
              190  GGCCC               
SmlI          557  CTCAAG               C^T[CT][GA]AG
              669  CTCAAG              


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AciI        AluI        BbvI        BcnI        BsaJI       
BsiYI       BslI        BsrI        BssKI       BstNI       
Cac8I       DdeI        EcoRII      Fnu4HI      HaeIII      
HapII       HpaII       ItaI        MaeIII      MboII       
MnlI        MspI        MvaI        MwoI        NciI        
NlaIV       ScrFI       TseI        
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