Restriction map for pF1KB6319
 (Linear) (Six-base) MAPSORT of: KIBB6319.seq  Check: 7423  from: 1  to: 780



KIBB6319.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     195     780 
   Size:        195     585
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     399     780 
   Size:        399     381
 
AlwNI CAG_nnn'CTG

Cuts at:      0     496     780 
   Size:        496     284
 
ApaI G_GGCC'C

Cuts at:      0     193     780 
   Size:        193     587
 
ApoI r'AATT_y

Cuts at:      0     400     780 
   Size:        400     380
 
AvaI C'yCGr_G

Cuts at:      0      26     780 
   Size:         26     754
 
BanI G'GyrC_C

Cuts at:      0     169     780 
   Size:        169     611
 
BanII G_rGCy'C

Cuts at:      0     193     780 
   Size:        193     587
 
BclI T'GATC_A

Cuts at:      0     648     780 
   Size:        648     132
 
Bme1580I G_kGCm'C

Cuts at:      0     193     780 
   Size:        193     587
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     468     780 
   Size:        468     312
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     473     780 
   Size:        280     193     307

  Fragments arranged by size: 

                 307     280     193
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     542     654     780 
   Size:        542     112     126

  Fragments arranged by size: 

                 542     126     112
 
BsaWI w'CCGG_w

Cuts at:      0      59     780 
   Size:         59     721
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     106     136     780 
   Size:        106      30     644

  Fragments arranged by size: 

                 644     106      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     114     780 
   Size:        114     666
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     780 
   Size:        331     449
 
BsmI GAATG_Cn'

Cuts at:      0     546     780 
   Size:        546     234
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      88     780 
   Size:         88     692
 
Bsp1286I G_dGCh'C

Cuts at:      0     193     780 
   Size:        193     587
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     195     780 
   Size:        195     585
 
BsrGI T'GTAC_A

Cuts at:      0     684     780 
   Size:        684      96
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     309     780 
   Size:        309     471
 
Bsu36I CC'TnA_GG

Cuts at:      0      84     588     780 
   Size:         84     504     192

  Fragments arranged by size: 

                 504     192      84
 
EaeI y'GGCC_r

Cuts at:      0     579     780 
   Size:        579     201
 
EarI CTCTTCn'nnn_

Cuts at:      0     757     780 
   Size:        757      23
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     473     780 
   Size:        280     193     307

  Fragments arranged by size: 

                 307     280     193
 
EcoO109I rG'GnC_Cy

Cuts at:      0     190     780 
   Size:        190     590
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     541     780 
   Size:        541     239
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     249     281     640     672     780 
   Size:        249      32     359      32     108

  Fragments arranged by size: 

                 359     249     108      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     780 
   Size:         46     734
 
MscI TGG'CCA

Cuts at:      0     581     780 
   Size:        581     199
 
NspI r_CATG'y

Cuts at:      0     718     780 
   Size:        718      62
 
PasI CC'CwG_GG

Cuts at:      0      50     780 
   Size:         50     730
 
PsiI TTA'TAA

Cuts at:      0     736     780 
   Size:        736      44
 
PspOMI G'GGCC_C

Cuts at:      0     189     780 
   Size:        189     591
 
SmlI C'TyrA_G

Cuts at:      0     557     669     780 
   Size:        557     112     111

  Fragments arranged by size: 

                 557     112     111
 
SspI AAT'ATT

Cuts at:      0     420     780 
   Size:        420     360
 
StyI C'CwwG_G

Cuts at:      0     230     780 
   Size:        230     550
 
TatI w'GTAC_w

Cuts at:      0     684     780 
   Size:        684      96
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     106     138     643     675     780 
   Size:        106      32     505      32     105

  Fragments arranged by size: 

                 505     106     105      32      32
 
XbaI T'CTAG_A

Cuts at:      0     273     780 
   Size:        273     507
 
XmnI GAAnn'nnTTC

Cuts at:      0      44     780 
   Size:         44     736
 

 Enzymes that do cut:

     AarI     AlfI    AlwNI     ApaI     ApoI     AvaI     BanI    BanII
     BclI Bme1580I     BmrI     BpmI    BpuEI    BsaWI    BsaXI    BseRI
     BsgI     BsmI    BsmBI Bsp1286I    BspMI    BsrGI   BstAPI   Bsu36I
     EaeI     EarI  Eco57MI EcoO109I     FalI    Hin4I     MmeI     MscI
     NspI     PasI     PsiI   PspOMI     SmlI     SspI     StyI     TatI
     TstI     XbaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BseYI    BsiEI  BsiHKAI    BsiWI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstBI
   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI    TaqII  Tth111I     XcmI     XhoI     XmaI
     ZraI
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