Restriction map for pF1KA0522
##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       Alw44I      
ApaLI       AscI        AseI        AsnI        Asp718I     
AspI        AviII       BbrPI       BclI        BfrI        
BsaAI       BsaBI       BsiEI       BsiWI       BsmI        
BspHI       Bst1107I    BstBI       BstZ17I     CpoI        
DraI        DraIII      DrdI        EagI        EclXI       
Eco52I      EcoRV       EcoT22I     FbaI        FspI        
HindIII     HpaI        KpnI        MamI        MfeI        
MluI        MseI        MunI        NheI        NotI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       ScaI        
SfiI        SfuI        SnaBI       SpeI        SspI        
SwaI        Tru9I       Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccI         2909  GTCGAC               GT^[AC][GT]AC
AhdI         2836  GACCATGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          912  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       941  AGCGCT               AGC^GCT
Asp700I      2433  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        2836  GACCATGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        2418  CCTAGG               C^CTAGG
BalI         3741  TGGCCA               TGG^CCA
BciVI        2312  GTATCC               GTATCC(6/5)
BglII        3041  AGATCT               A^GATCT
BlnI         2418  CCTAGG               C^CTAGG
BspDI        1318  ATCGAT               AT^CGAT
BspLU11I     2681  ACATGT               A^CATGT
BsrDI        3237  CATTGC               GCAATG(2/0)
BssHII        233  GCGCGC               G^CGCGC
BstAPI         99  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       3015  GGTCACC              G^GT[ACGT]ACC
BstPI        3015  GGTCACC              G^GT[ACGT]ACC
Bsu36I        912  CCTGAGG              CC^T[ACGT]AGG
ClaI         1318  ATCGAT               AT^CGAT
Eam1105I     2836  GACCATGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      941  AGCGCT               AGC^GCT
Eco81I        912  CCTGAGG              CC^T[ACGT]AGG
EcoNI         180  CCTGCGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      3015  GGTCACC              G^GT[ACGT]ACC
MluNI        3741  TGGCCA               TGG^CCA
MscI         3741  TGGCCA               TGG^CCA
NdeI         1292  CATATG               CA^TATG
NruI          501  TCGCGA               TCG^CGA
PflMI        2550  CCAGAAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI         2909  GTCGAC               G^TCGAC
SapI         3099  GCTCTTC              GCTCTTC(1/4)
SbfI          984  CCTGCAGG             CCTGCA^GG
SexAI        3150  ACCTGGT              A^CC[AT]GGT
SphI         3801  GCATGC               GCATG^C
Sse8387I      984  CCTGCAGG             CCTGCA^GG
Van91I       2550  CCAGAAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         2433  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccIII       2510  TCCGGA               T^CCGGA
             2624  TCCGGA              
AflIII       2450  ACGTGT               A^C[GA][CT]GT
             2681  ACATGT              
AgeI          433  ACCGGT               A^CCGGT
             2352  ACCGGT              
BamHI          34  GGATCC               G^GATCC
             4219  GGATCC              
BbvCI        2391  CCTCAGC              CCTCAGC(-5/-2)
             3932  CCTCAGC             
BcgI         2911  CGACTGGTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3231  CGAGTCCATTGC        
BseAI        2510  TCCGGA               T^CCGGA
             2624  TCCGGA              
BsmBI        3591  CGTCTC               CGTCTC(1/5)
             2447  GAGACG              
Bsp1407I     2684  TGTACA               T^GTACA
             4109  TGTACA              
BspEI        2510  TCCGGA               T^CCGGA
             2624  TCCGGA              
BsrGI        2684  TGTACA               T^GTACA
             4109  TGTACA              
FseI         1621  GGCCGGCC             GGCCGG^CC
             4009  GGCCGGCC            
HgaI          406  GACGC                GACGC(5/10)
             2696  GCGTC               
HincII       2752  GTTGAC               GT[CT]^[GA]AC
             2909  GTCGAC              
HindII       2752  GTTGAC               GT[CT]^[GA]AC
             2909  GTCGAC              
MroI         2510  TCCGGA               T^CCGGA
             2624  TCCGGA              
PinAI         433  ACCGGT               A^CCGGT
             2352  ACCGGT              
SacI          633  GAGCTC               GAGCT^C
             2100  GAGCTC              
SgrAI         648  CGCCGGCG             C[GA]^CCGG[CT]G
             2351  CACCGGTG            
SmlI         1378  CTTGAG               C^T[CT][GA]AG
             2095  CTTGAG              
SspBI        2684  TGTACA               T^GTACA
             4109  TGTACA              
StuI         2388  AGGCCT               AGG^CCT
             3680  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  128 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        ApoI        
AspHI       AvaI        AvaII       BanI        BanII       
BbeI        BbsI        BbvI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaHI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BssSI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRI       EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PleI        PpuMI       
PstI        PvuII       RsaI        SacII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmaI        StyI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII       XmaCI       XmaI        
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