Restriction map for pF1KA0522
 (Linear) (Six-base) MAPSORT of: KIAA0522.seq  Check: 3091  from: 1  to: 4464



KIAA0522.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 16:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     482    4464 
   Size:        482    3982
 
AccI GT'mk_AC

Cuts at:      0    2910    4464 
   Size:       2910    1554
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1521    1915    1973    2562    4152    4464 
   Size:       1521     394      58     589    1590     312

  Fragments arranged by size: 

                1590    1521     589     394     312      58
 
AfeI AGC'GCT

Cuts at:      0     943    4464 
   Size:        943    3521
 
AflIII A'CryG_T

Cuts at:      0    2450    2681    4464 
   Size:       2450     231    1783

  Fragments arranged by size: 

                2450    1783     231
 
AgeI A'CCGG_T

Cuts at:      0     433    2352    4464 
   Size:        433    1919    2112

  Fragments arranged by size: 

                2112    1919     433
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2841    4464 
   Size:       2841    1623
 
AleI CACnn'nnGTG

Cuts at:      0    2353    4464 
   Size:       2353    2111
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3445    4464 
   Size:       3445    1019
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     301     333    1271    1303    4464 
   Size:        301      32     938      32    3161

  Fragments arranged by size: 

                3161     938     301      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     105     108     273    1951    2322    3151    3798    4464 
   Size:        105       3     165    1678     371     829     647     666

  Fragments arranged by size: 

                1678     829     666     647     371     165     105       3
            
 
ApaI G_GGCC'C

Cuts at:      0      20     850    1457    1923    1982    2015    3650    3829 
   Size:         20     830     607     466      59      33    1635     179

Cuts at:   3829    3992    4153    4216    4397    4464 
   Size:        163     161      63     181      67

  Fragments arranged by size: 

                1635     830     607     466     181     179     163     161
                  67      63      59      33      20
 
ApoI r'AATT_y

Cuts at:      0     720    2413    3023    3108    4464 
   Size:        720    1693     610      85    1356

  Fragments arranged by size: 

                1693    1356     720     610      85
 
AvaI C'yCGr_G

Cuts at:      0      21      30     246     332     537    1627    1685    1726 
   Size:         21       9     216      86     205    1090      58      41

Cuts at:   1726    2150    2774    4464 
   Size:        424     624    1690

  Fragments arranged by size: 

                1690    1090     624     424     216     205      86      58
                  41      21       9
 
AvrII C'CTAG_G

Cuts at:      0    2418    4464 
   Size:       2418    2046
 
BamHI G'GATC_C

Cuts at:      0      34    4219    4464 
   Size:         34    4185     245

  Fragments arranged by size: 

                4185     245      34
 
BanI G'GyrC_C

Cuts at:      0     361     646    1246    1258    1638    2940    4023    4464 
   Size:        361     285     600      12     380    1302    1083     441

  Fragments arranged by size: 

                1302    1083     600     441     380     361     285      12
            
 
BanII G_rGCy'C

Cuts at:      0      20      46     637     850     879    1030    1289    1457 
   Size:         20      26     591     213      29     151     259     168

Cuts at:   1457    1923    1982    2015    2104    3404    3619    3650    3829 
   Size:        466      59      33      89    1300     215      31     179

Cuts at:   3829    3913    3992    4153    4216    4240    4397    4464 
   Size:         84      79     161      63      24     157      67

  Fragments arranged by size: 

                1300     591     466     259     215     213     179     168
                 161     157     151      89      84      79      67      63
                  59      33      31      29      26      24      20
 
BbeI G_GCGC'C

Cuts at:      0     365     650    1642    4464 
   Size:        365     285     992    2822

  Fragments arranged by size: 

                2822     992     365     285
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1697    2771    2982    4464 
   Size:       1697    1074     211    1482

  Fragments arranged by size: 

                1697    1482    1074     211
 
BbvCI CC'TCA_GC

Cuts at:      0    2392    3933    4464 
   Size:       2392    1541     531

  Fragments arranged by size: 

                2392    1541     531
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2900    2934    3220    3254    4464 
   Size:       2900      34     286      34    1210

  Fragments arranged by size: 

                2900    1210     286      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2323    4464 
   Size:       2323    2141
 
BglI GCCn_nnn'nGGC

Cuts at:      0     529    3755    3777    4016    4464 
   Size:        529    3226      22     239     448

  Fragments arranged by size: 

                3226     529     448     239      22
 
BglII A'GATC_T

Cuts at:      0    3041    4464 
   Size:       3041    1423
 
BlpI GC'TnA_GC

Cuts at:      0     613     811     878    1116    1524    4464 
   Size:        613     198      67     238     408    2940

  Fragments arranged by size: 

                2940     613     408     238     198      67
 
Bme1580I G_kGCm'C

Cuts at:      0      20     850     888    1263    1457    1923    1982    2015 
   Size:         20     830      38     375     194     466      59      33

Cuts at:   2015    3650    3829    3992    4026    4153    4216    4397    4464 
   Size:       1635     179     163      34     127      63     181      67

  Fragments arranged by size: 

                1635     830     466     375     194     181     179     163
                 127      67      63      59      38      34      33      20
            
 
BmgBI CAC'GTC

Cuts at:      0     339     459    2451    4464 
   Size:        339     120    1992    2013

  Fragments arranged by size: 

                2013    1992     339     120
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     279     459    2009    2018    3801    3973    4257    4412 
   Size:        279     180    1550       9    1783     172     284     155

Cuts at:   4412    4464 
   Size:         52

  Fragments arranged by size: 

                1783    1550     284     279     180     172     155      52
                   9
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1539    4464 
   Size:       1539    2925
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     160     406     621     682     813     877     878 
   Size:         91      69     246     215      61     131      64       1

Cuts at:    878    1030    1370    1507    2088    2398    3181    3304    3347 
   Size:        152     340     137     581     310     783     123      43

Cuts at:   3347    3394    3775    4061    4118    4464 
   Size:         47     381     286      57     346

  Fragments arranged by size: 

                 783     581     381     346     340     310     286     246
                 215     152     137     131     123      91      69      64
                  61      57      47      43       1
 
Bpu10I CC'TnA_GC

Cuts at:      0     766    1036    1586    2392    3933    4154    4464 
   Size:        766     270     550     806    1541     221     310

  Fragments arranged by size: 

                1541     806     766     550     310     270     221
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1399    2116    4464 
   Size:       1399     717    2348

  Fragments arranged by size: 

                2348    1399     717
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     274    2113    2771    3428    4464 
   Size:        274    1839     658     657    1036

  Fragments arranged by size: 

                1839    1036     658     657     274
 
BsaHI Gr'CG_yC

Cuts at:      0     362     407     647    1639    4464 
   Size:        362      45     240     992    2825

  Fragments arranged by size: 

                2825     992     362     240      45
 
BsaWI w'CCGG_w

Cuts at:      0     433    2352    2510    2624    4464 
   Size:        433    1919     158     114    1840

  Fragments arranged by size: 

                1919    1840     433     158     114
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      25     301     331     370     400     624     654    1003 
   Size:         25     276      30      39      30     224      30     349

Cuts at:   1003    1033    1271    1301    1375    1405    1459    1489    2119 
   Size:         30     238      30      74      30      54      30     630

Cuts at:   2119    2149    2470    2500    3830    3860    4464 
   Size:         30     321      30    1330      30     604

  Fragments arranged by size: 

                1330     630     604     349     321     276     238     224
                  74      54      39      30      30      30      30      30
                  30      30      30      30      30      25
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     148     202     940     943     946    1198    1480    2133 
   Size:        148      54     738       3       3     252     282     653

Cuts at:   2133    2475    2659    3583    3591    3681    4464 
   Size:        342     184     924       8      90     783

  Fragments arranged by size: 

                 924     783     738     653     342     282     252     184
                 148      90      54       8       3       3
 
BseYI C'CCAG_C

Cuts at:      0     116     489    1481    1597    1944    1959    1965    2026 
   Size:        116     373     992     116     347      15       6      61

Cuts at:   2026    2222    2604    2692    3190    3854    3881    3902    3906 
   Size:        196     382      88     498     664      27      21       4

Cuts at:   3906    4124    4207    4464 
   Size:        218      83     257

  Fragments arranged by size: 

                 992     664     498     382     373     347     257     218
                 196     116     116      88      83      61      27      21
                  15       6       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     199     460    2872    3268    4066    4366    4464 
   Size:        184      15     261    2412     396     798     300      98

  Fragments arranged by size: 

                2412     798     396     300     261     184      98      15
            
 
BsiHKAI G_wGCw'C

Cuts at:      0     637     772    2104    4464 
   Size:        637     135    1332    2360

  Fragments arranged by size: 

                2360    1332     637     135
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2441    3597    4464 
   Size:       2441    1156     867

  Fragments arranged by size: 

                2441    1156     867
 
Bsp1286I G_dGCh'C

Cuts at:      0      20      46     637     772     850     879     888    1030 
   Size:         20      26     591     135      78      29       9     142

Cuts at:   1030    1263    1289    1457    1923    1982    2015    2104    3404 
   Size:        233      26     168     466      59      33      89    1300

Cuts at:   3404    3619    3650    3829    3913    3992    4026    4153    4216 
   Size:        215      31     179      84      79      34     127      63

Cuts at:   4216    4240    4397    4464 
   Size:         24     157      67

  Fragments arranged by size: 

                1300     591     466     233     215     179     168     157
                 142     135     127      89      84      79      78      67
                  63      59      34      33      31      29      26      26
                  24      20       9
 
BspEI T'CCGG_A

Cuts at:      0    2510    2624    4464 
   Size:       2510     114    1840

  Fragments arranged by size: 

                2510    1840     114
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      74     188     281     482     992    3233    4464 
   Size:         74     114      93     201     510    2241    1231

  Fragments arranged by size: 

                2241    1231     510     201     114      93      74
 
BsrBI CCG'CTC

Cuts at:      0     402     420     603    1104    1826    2331    2382    4464 
   Size:        402      18     183     501     722     505      51    2082

  Fragments arranged by size: 

                2082     722     505     501     402     183      51      18
            
 
BsrDI GCAATG_nn'

Cuts at:      0    3236    4464 
   Size:       3236    1228
 
BsrFI r'CCGG_y

Cuts at:      0     433     528     649     806    1271    1622    2179    2352 
   Size:        433      95     121     157     465     351     557     173

Cuts at:   2352    2396    2426    3203    4010    4464 
   Size:         44      30     777     807     454

  Fragments arranged by size: 

                 807     777     557     465     454     433     351     173
                 157     121      95      44      30
 
BsrGI T'GTAC_A

Cuts at:      0    2684    4109    4464 
   Size:       2684    1425     355

  Fragments arranged by size: 

                2684    1425     355
 
BssHII G'CGCG_C

Cuts at:      0     233    4464 
   Size:        233    4231
 
BssSI C'ACGA_G

Cuts at:      0     481    2611    3079    4464 
   Size:        481    2130     468    1385

  Fragments arranged by size: 

                2130    1385     481     468
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     105    4464 
   Size:        105    4359
 
BstEII G'GTnAC_C

Cuts at:      0    3015    4464 
   Size:       3015    1449
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2022    2496    3739    3973    4464 
   Size:       2022     474    1243     234     491

  Fragments arranged by size: 

                2022    1243     491     474     234
 
BstYI r'GATC_y

Cuts at:      0      34    2493    2946    3041    4219    4464 
   Size:         34    2459     453      95    1178     245

  Fragments arranged by size: 

                2459    1178     453     245      95      34
 
Bsu36I CC'TnA_GG

Cuts at:      0     913    4464 
   Size:        913    3551
 
BtgI C'CryG_G

Cuts at:      0      56     204     228     586     595     618    1250    1808 
   Size:         56     148      24     358       9      23     632     558

Cuts at:   1808    1884    2489    3750    3972    4371    4457    4464 
   Size:         76     605    1261     222     399      86       7

  Fragments arranged by size: 

                1261     632     605     558     399     358     222     148
                  86      76      56      24      23       9       7
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3410    3940    4464 
   Size:       3410     530     524

  Fragments arranged by size: 

                3410     530     524
 
BtsI GCAGTG_nn'

Cuts at:      0     765    4099    4464 
   Size:        765    3334     365

  Fragments arranged by size: 

                3334     765     365
 
ClaI AT'CG_AT

Cuts at:      0    1319    4464 
   Size:       1319    3145
 
EaeI y'GGCC_r

Cuts at:      0     589     686    3741    4012    4068    4225    4464 
   Size:        589      97    3055     271      56     157     239

  Fragments arranged by size: 

                3055     589     271     239     157      97      56
 
EarI CTCTTCn'nnn_

Cuts at:      0    2151    2293    3001    3106    3699    3872    4464 
   Size:       2151     142     708     105     593     173     592

  Fragments arranged by size: 

                2151     708     593     592     173     142     105
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     668    1063    3536    4464 
   Size:        668     395    2473     928

  Fragments arranged by size: 

                2473     928     668     395
 
EcoICRI GAG'CTC

Cuts at:      0     635    2102    4464 
   Size:        635    1467    2362

  Fragments arranged by size: 

                2362    1467     635
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     160     406     621     682     813     877     878 
   Size:         91      69     246     215      61     131      64       1

Cuts at:    878    1030    1370    1507    1521    1915    1973    2088    2398 
   Size:        152     340     137      14     394      58     115     310

Cuts at:   2398    2562    3181    3304    3347    3394    3775    4061    4118 
   Size:        164     619     123      43      47     381     286      57

Cuts at:   4118    4152    4464 
   Size:         34     312

  Fragments arranged by size: 

                 619     394     381     340     312     310     286     246
                 215     164     152     137     131     123     115      91
                  69      64      61      58      57      47      43      34
                  14       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     184    4464 
   Size:        184    4280
 
EcoO109I rG'GnC_Cy

Cuts at:      0      16      17     222     846     847     951    1453    1723 
   Size:         16       1     205     624       1     104     502     270

Cuts at:   1723    1889    1919    1920    1978    1979    3646    3647    3795 
   Size:        166      30       1      58       1    1667       1     148

Cuts at:   3795    3826    3988    3989    4149    4150    4212    4213    4394 
   Size:         31     162       1     160       1      62       1     181

Cuts at:   4394    4464 
   Size:         70

  Fragments arranged by size: 

                1667     624     502     270     205     181     166     162
                 160     148     104      70      62      58      31      30
                  16       1       1       1       1       1       1       1
                   1
 
EcoRI G'AATT_C

Cuts at:      0     720    2413    3108    4464 
   Size:        720    1693     695    1356

  Fragments arranged by size: 

                1693    1356     720     695
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3060    3666    4464 
   Size:       3060     606     798

  Fragments arranged by size: 

                3060     798     606
 
FseI GG_CCGG'CC

Cuts at:      0    1626    4014    4464 
   Size:       1626    2388     450

  Fragments arranged by size: 

                2388    1626     450
 
HaeII r_GCGC'y

Cuts at:      0     365     650     945    1642    4464 
   Size:        365     285     295     697    2822

  Fragments arranged by size: 

                2822     697     365     295     285
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      27      33      65     624     656    1375    1407    1459 
   Size:         27       6      32     559      32     719      32      52

Cuts at:   1459    1491    1822    1854    2470    2502    2827    2859    3528 
   Size:         32     331      32     616      32     325      32     669

Cuts at:   3528    3560    3766    3798    4464 
   Size:         32     206      32     666

  Fragments arranged by size: 

                 719     669     666     616     559     331     325     206
                  52      32      32      32      32      32      32      32
                  32      32      27       6
 
HincII GTy'rAC

Cuts at:      0    2754    2911    4464 
   Size:       2754     157    1553

  Fragments arranged by size: 

                2754    1553     157
 
KasI G'GCGC_C

Cuts at:      0     361     646    1638    4464 
   Size:        361     285     992    2826

  Fragments arranged by size: 

                2826     992     361     285
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1181    2014    2435    2856    3245    3257    3620    4464 
   Size:       1181     833     421     421     389      12     363     844

  Fragments arranged by size: 

                1181     844     833     421     421     389     363      12
            
 
MscI TGG'CCA

Cuts at:      0    3743    4464 
   Size:       3743     721
 
MslI CAynn'nnrTG

Cuts at:      0    1165    2353    3737    3971    4464 
   Size:       1165    1188    1384     234     493

  Fragments arranged by size: 

                1384    1188    1165     493     234
 
MspA1I CmG'CkG

Cuts at:      0     105     120     150     198     206     588     597     612 
   Size:        105      15      30      48       8     382       9      15

Cuts at:    612     775     819     882    1485    1854    2205    2586    2982 
   Size:        163      44      63     603     369     351     381     396

Cuts at:   2982    3906    4380    4464 
   Size:        924     474      84

  Fragments arranged by size: 

                 924     603     474     396     382     381     369     351
                 163     105      84      63      48      44      30      15
                  15       9       8
 
NaeI GCC'GGC

Cuts at:      0     530     651    1624    2398    4012    4464 
   Size:        530     121     973     774    1614     452

  Fragments arranged by size: 

                1614     973     774     530     452     121
 
NarI GG'CG_CC

Cuts at:      0     362     647    1639    4464 
   Size:        362     285     992    2825

  Fragments arranged by size: 

                2825     992     362     285
 
NcoI C'CATG_G

Cuts at:      0    1250    1884    2489    3972    4371    4464 
   Size:       1250     634     605    1483     399      93

  Fragments arranged by size: 

                1483    1250     634     605     399      93
 
NdeI CA'TA_TG

Cuts at:      0    1293    4464 
   Size:       1293    3171
 
NgoMIV G'CCGG_C

Cuts at:      0     528     649    1622    2396    4010    4464 
   Size:        528     121     973     774    1614     454

  Fragments arranged by size: 

                1614     973     774     528     454     121
 
NruI TCG'CGA

Cuts at:      0     503    4464 
   Size:        503    3961
 
NspI r_CATG'y

Cuts at:      0    1137    1164    2685    3096    3805    4464 
   Size:       1137      27    1521     411     709     659

  Fragments arranged by size: 

                1521    1137     709     659     411      27
 
PasI CC'CwG_GG

Cuts at:      0     495     872    1617    3985    4464 
   Size:        495     377     745    2368     479

  Fragments arranged by size: 

                2368     745     495     479     377
 
PciI A'CATG_T

Cuts at:      0    2681    4464 
   Size:       2681    1783
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2556    4464 
   Size:       2556    1908
 
PfoI T'CCnGG_A

Cuts at:      0     422     628    2375    3197    4464 
   Size:        422     206    1747     822    1267

  Fragments arranged by size: 

                1747    1267     822     422     206
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     271     301     303     333    1271    1303    4464 
   Size:        271      30       2      30     938      32    3161

  Fragments arranged by size: 

                3161     938     271      32      30      30       2
 
PpuMI rG'GwC_Cy

Cuts at:      0     222    1723    1889    4464 
   Size:        222    1501     166    2575

  Fragments arranged by size: 

                2575    1501     222     166
 
PspOMI G'GGCC_C

Cuts at:      0      16     846    1453    1919    1978    2011    3646    3825 
   Size:         16     830     607     466      59      33    1635     179

Cuts at:   3825    3988    4149    4212    4393    4464 
   Size:        163     161      63     181      71

  Fragments arranged by size: 

                1635     830     607     466     181     179     163     161
                  71      63      59      33      16
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1195    1227    4464 
   Size:       1195      32    3237

  Fragments arranged by size: 

                3237    1195      32
 
PstI C_TGCA'G

Cuts at:      0     989    1879    2134    3147    4464 
   Size:        989     890     255    1013    1317

  Fragments arranged by size: 

                1317    1013     989     890     255
 
PvuII CAG'CTG

Cuts at:      0     105     150     198     612     819    1485    1854    3906 
   Size:        105      45      48     414     207     666     369    2052

Cuts at:   3906    4464 
   Size:        558

  Fragments arranged by size: 

                2052     666     558     414     369     207     105      48
                  45
 
SacI G_AGCT'C

Cuts at:      0     637    2104    4464 
   Size:        637    1467    2360

  Fragments arranged by size: 

                2360    1467     637
 
SacII CC_GC'GG

Cuts at:      0     207     589     598    4464 
   Size:        207     382       9    3866

  Fragments arranged by size: 

                3866     382     207       9
 
SalI G'TCGA_C

Cuts at:      0    2909    4464 
   Size:       2909    1555
 
SanDI GG'GwC_CC

Cuts at:      0     222    1723    1889    4464 
   Size:        222    1501     166    2575

  Fragments arranged by size: 

                2575    1501     222     166
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3106    4464 
   Size:       3106    1358
 
SbfI CC_TGCA'GG

Cuts at:      0     989    4464 
   Size:        989    3475
 
SexAI A'CCwGG_T

Cuts at:      0    3150    4464 
   Size:       3150    1314
 
SfcI C'TryA_G

Cuts at:      0     985    1875    2130    3143    4464 
   Size:        985     890     255    1013    1321

  Fragments arranged by size: 

                1321    1013     985     890     255
 
SfoI GGC'GCC

Cuts at:      0     363     648    1640    4464 
   Size:        363     285     992    2824

  Fragments arranged by size: 

                2824     992     363     285
 
SgrAI Cr'CCGG_yG

Cuts at:      0     649    2352    4464 
   Size:        649    1703    2112

  Fragments arranged by size: 

                2112    1703     649
 
SmaI CCC'GGG

Cuts at:      0      23      32     248     334     539    1687    1728    4464 
   Size:         23       9     216      86     205    1148      41    2736

  Fragments arranged by size: 

                2736    1148     216     205      86      41      23       9
            
 
SmlI C'TyrA_G

Cuts at:      0    1378    2095    4464 
   Size:       1378     717    2369

  Fragments arranged by size: 

                2369    1378     717
 
SphI G_CATG'C

Cuts at:      0    3805    4464 
   Size:       3805     659
 
SrfI GCCC'GGGC

Cuts at:      0     248    4464 
   Size:        248    4216
 
StuI AGG'CCT

Cuts at:      0    2390    3682    4464 
   Size:       2390    1292     782

  Fragments arranged by size: 

                2390    1292     782
 
StyI C'CwwG_G

Cuts at:      0     947    1250    1884    2418    2489    3335    3816    3972 
   Size:        947     303     634     534      71     846     481     156

Cuts at:   3972    4004    4371    4430    4464 
   Size:         32     367      59      34

  Fragments arranged by size: 

                 947     846     634     534     481     367     303     156
                  71      59      34      32
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     178    4041    4193    4302    4374    4464 
   Size:        178    3863     152     109      72      90

  Fragments arranged by size: 

                3863     178     152     109      90      72
 
TatI w'GTAC_w

Cuts at:      0    2684    3572    4109    4464 
   Size:       2684     888     537     355

  Fragments arranged by size: 

                2684     888     537     355
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3830    3862    4464 
   Size:       3830      32     602

  Fragments arranged by size: 

                3830     602      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     616     837    3736    3751    4464 
   Size:        616     221    2899      15     713

  Fragments arranged by size: 

                2899     713     616     221      15
 
XmaI C'CCGG_G

Cuts at:      0      21      30     246     332     537    1685    1726    4464 
   Size:         21       9     216      86     205    1148      41    2738

  Fragments arranged by size: 

                2738    1148     216     205      86      41      21       9
            
 
XmnI GAAnn'nnTTC

Cuts at:      0    2437    4464 
   Size:       2437    2027
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI     ApoI     AvaI    AvrII    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI    HaeII    Hin4I
   HincII     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NruI     NspI     PasI     PciI    PflMI
     PfoI     PpiI    PpuMI   PspOMI     PsrI     PstI    PvuII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfcI     SfoI
    SgrAI     SmaI     SmlI     SphI     SrfI     StuI     StyI    TaqII
     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII    ApaLI     AscI     AseI    AsiSI
     BaeI     BclI    BfrBI     BmtI    BsaAI    BsaBI    BsiEI    BsiWI
     BsmI    BspHI    BstBI  BstZ17I    CspCI     DraI   DraIII     DrdI
     EagI    EcoRV     FspI    FspAI  HindIII     HpaI     KpnI     MfeI
     MluI     NheI     NotI     NsiI     PacI     PmeI     PmlI    PshAI
     PsiI    PspXI     PvuI    RsrII     ScaI     SfiI     SgfI    SnaBI
     SpeI     SspI     SwaI  Tth111I     XbaI     XhoI     ZraI
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