Restriction map for pF1KA1023
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflII       
AflIII      AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BalI        BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BseAI       BsiWI       BsmI        BspDI       BspEI       
BspHI       BspLU11I    BspMI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
ClaI        DraI        DraIII      Eam1105I    Eco47III    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        KpnI        
MaeI        MaeIII      MfeI        MluI        MluNI       
MroI        MscI        MslI        MunI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PmeI        PshBI       Psp1406I    PvuI        
RcaI        SalI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SspI        SwaI        TaqI        Tsp45I      Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII        1822  GACGTC               GACGT^C
AccI          220  GTCTAC               GT^[AC][GT]AC
AfaI         1401  GTAC                 GT^AC
AgeI         1675  ACCGGT               A^CCGGT
AocI         1784  CCTCAGG              CC^T[ACGT]AGG
AvaI         1655  CCCGGG               C^[CT]CG[GA]G
BbrPI         277  CACGTG               CAC^GTG
BbvCI         822  GCTGAGG              CCTCAGC(-5/-2)
BlpI          759  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      759  GCTGAGC              GC^T[ACGT]AGC
BsaAI         277  CACGTG               [CT]AC^GT[GA]
BsaBI         546  GATGCGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          998  GAGACC               GGTCTC(1/5)
BsiEI          12  CGGCCG               CG[GA][CT]^CG
BsoBI        1655  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     1400  TGTACA               T^GTACA
BsrGI        1400  TGTACA               T^GTACA
Bsu36I       1784  CCTCAGG              CC^T[ACGT]AGG
CelII         759  GCTGAGC              GC^T[ACGT]AGC
CpoI         1015  CGGACCG              CG^G[AT]CCG
DrdI         1843  GACGATATTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           12  CGGCCG               C^GGCCG
EclXI          12  CGGCCG               C^GGCCG
Eco52I         12  CGGCCG               C^GGCCG
Eco81I       1784  CCTCAGG              CC^T[ACGT]AGG
HincII       1825  GTCAAC               GT[CT]^[GA]AC
HindII       1825  GTCAAC               GT[CT]^[GA]AC
MamI          546  GATGCGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI          557  CCATGG               C^CATGG
NspI          105  GCATGC               [GA]CATG^[CT]
PinAI        1675  ACCGGT               A^CCGGT
PmaCI         277  CACGTG               CAC^GTG
PmlI          277  CACGTG               CAC^GTG
PpuMI         743  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII        1033  CAGCTG               CAG^CTG
RsaI         1401  GTAC                 GT^AC
RsrII        1015  CGGACCG              CG^G[AT]CCG
SmaI         1655  CCCGGG               CCC^GGG
SphI          105  GCATGC               GCATG^C
SspBI        1400  TGTACA               T^GTACA
Tsp509I      1190  AATT                 ^AATT
XmaCI        1655  CCCGGG               C^CCGGG
XmaI         1655  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

BamHI         372  GGATCC               G^GATCC
              458  GGATCC              
BbeI          320  GGCGCC               GGCGC^C
              652  GGCGCC              
BbsI          525  GAAGAC               GAAGAC(2/6)
              645  GAAGAC              
BpuAI         525  GAAGAC               GAAGAC(2/6)
              645  GAAGAC              
BsaWI         619  ACCGGA               [AT]^CCGG[AT]
             1675  ACCGGT              
BsrDI         272  CATTGC               GCAATG(2/0)
             1854  CATTGC              
BsrFI         350  ACCGGC               [GA]^CCGG[CT]
             1675  ACCGGT              
BssSI         950  CACGAG               CACGAG(-5/-1)
              973  CACGAG              
Cfr10I        350  ACCGGC               [GA]^CCGG[CT]
             1675  ACCGGT              
DraII          77  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              743  AGGACCT             
EcoO109I       77  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              743  AGGACCT             
EcoT14I       557  CCATGG               C^C[AT][AT]GG
             1799  CCTTGG              
KasI          320  GGCGCC               G^GCGCC
              652  GGCGCC              
MseI          262  TTAA                 T^TAA
              440  TTAA                
NarI          320  GGCGCC               GG^CGCC
              652  GGCGCC              
PshAI         114  GACTGGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
              213  GACTCCTGTC          
SbfI         1068  CCTGCAGG             CCTGCA^GG
             1380  CCTGCAGG            
SfoI          320  GGCGCC               GGC^GCC
              652  GGCGCC              
Sse8387I     1068  CCTGCAGG             CCTGCA^GG
             1380  CCTGCAGG            
StuI         1533  AGGCCT               AGG^CCT
             1796  AGGCCT              
StyI          557  CCATGG               C^C[AT][AT]GG
             1799  CCTTGG              
Tru9I         262  TTAA                 T^TAA
              440  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   87 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
AlwNI       AspHI       AvaII       BanI        BanII       
BbvI        BcnI        BmyI        BpmI        BsaHI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       Bsp1286I    
BsrBI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DsaI        EaeI        
EarI        EcoNI       EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     KspI        MaeII       
MboI        MboII       MflI        MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PstI        
SacI        SacII       SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TfiI        TseI        
TspRI       XhoII       
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