Restriction map for pF1KA1023
 (Linear) (Six-base) MAPSORT of: KIAA1023.seq  Check: 8233  from: 1  to: 1890



KIAA1023.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:24  ..

AatII G_ACGT'C

Cuts at:      0    1826    1890 
   Size:       1826      64
 
AccI GT'mk_AC

Cuts at:      0     221    1890 
   Size:        221    1669
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141     469     484     757    1024    1435    1706    1890 
   Size:        141     328      15     273     267     411     271     184

  Fragments arranged by size: 

                 411     328     273     271     267     184     141      15
            
 
AgeI A'CCGG_T

Cuts at:      0    1675    1890 
   Size:       1675     215
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1618    1890 
   Size:       1618     272
 
AlwNI CAG_nnn'CTG

Cuts at:      0      39      81     369     735    1038    1068    1283    1890 
   Size:         39      42     288     366     303      30     215     607

  Fragments arranged by size: 

                 607     366     303     288     215      42      39      30
            
 
AvaI C'yCGr_G

Cuts at:      0    1655    1890 
   Size:       1655     235
 
BamHI G'GATC_C

Cuts at:      0     372     458    1890 
   Size:        372      86    1432

  Fragments arranged by size: 

                1432     372      86
 
BanI G'GyrC_C

Cuts at:      0     320     496     652    1803    1890 
   Size:        320     176     156    1151      87

  Fragments arranged by size: 

                1151     320     176     156      87
 
BanII G_rGCy'C

Cuts at:      0     716     871    1256    1325    1552    1576    1890 
   Size:        716     155     385      69     227      24     314

  Fragments arranged by size: 

                 716     385     314     227     155      69      24
 
BbeI G_GCGC'C

Cuts at:      0     324     656    1890 
   Size:        324     332    1234

  Fragments arranged by size: 

                1234     332     324
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     532     652    1890 
   Size:        532     120    1238

  Fragments arranged by size: 

                1238     532     120
 
BbvCI CC'TCA_GC

Cuts at:      0     823    1890 
   Size:        823    1067
 
BlpI GC'TnA_GC

Cuts at:      0     760    1890 
   Size:        760    1130
 
Bme1580I G_kGCm'C

Cuts at:      0     684    1562    1647    1890 
   Size:        684     878      85     243

  Fragments arranged by size: 

                 878     684     243      85
 
BmgBI CAC'GTC

Cuts at:      0     294    1890 
   Size:        294    1596
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1383    1890 
   Size:       1383     507
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     346     496     574     862    1108    1120    1890 
   Size:        346     150      78     288     246      12     770

  Fragments arranged by size: 

                 770     346     288     246     150      78      12
 
Bpu10I CC'TnA_GC

Cuts at:      0     688     823    1890 
   Size:        688     135    1067

  Fragments arranged by size: 

                1067     688     135
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     992    1890 
   Size:        992     898
 
BsaAI yAC'GTr

Cuts at:      0     279    1890 
   Size:        279    1611
 
BsaBI GATnn'nnATC

Cuts at:      0     550    1890 
   Size:        550    1340
 
BsaHI Gr'CG_yC

Cuts at:      0     119     321     653    1364    1739    1823    1890 
   Size:        119     202     332     711     375      84      67

  Fragments arranged by size: 

                 711     375     332     202     119      84      67
 
BsaWI w'CCGG_w

Cuts at:      0     619    1675    1890 
   Size:        619    1056     215

  Fragments arranged by size: 

                1056     619     215
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      94     124    1087    1117    1729    1759    1813    1843 
   Size:         94      30     963      30     612      30      54      30

Cuts at:   1843    1890 
   Size:         47

  Fragments arranged by size: 

                 963     612      94      54      47      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     343     589    1135    1138    1143    1156    1171    1330 
   Size:        343     246     546       3       5      13      15     159

Cuts at:   1330    1624    1725    1734    1890 
   Size:        294     101       9     156

  Fragments arranged by size: 

                 546     343     294     246     159     156     101      15
                  13       9       5       3
 
BseYI C'CCAG_C

Cuts at:      0      45     376    1257    1270    1522    1890 
   Size:         45     331     881      13     252     368

  Fragments arranged by size: 

                 881     368     331     252      45      13
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     301     922    1624    1890 
   Size:        301     621     702     266

  Fragments arranged by size: 

                 702     621     301     266
 
BsiEI CG_ry'CG

Cuts at:      0      15    1890 
   Size:         15    1875
 
BsiHKAI G_wGCw'C

Cuts at:      0     716     766     871    1256    1552    1890 
   Size:        716      50     105     385     296     338

  Fragments arranged by size: 

                 716     385     338     296     105      50
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     592     985    1355    1868    1890 
   Size:        592     393     370     513      22

  Fragments arranged by size: 

                 592     513     393     370      22
 
Bsp1286I G_dGCh'C

Cuts at:      0     684     716     766     871    1256    1325    1552    1562 
   Size:        684      32      50     105     385      69     227      10

Cuts at:   1562    1576    1647    1890 
   Size:         14      71     243

  Fragments arranged by size: 

                 684     385     243     227     105      71      69      50
                  32      14      10
 
BsrBI CCG'CTC

Cuts at:      0    1014    1337    1636    1890 
   Size:       1014     323     299     254

  Fragments arranged by size: 

                1014     323     299     254
 
BsrDI GCAATG_nn'

Cuts at:      0     271    1853    1890 
   Size:        271    1582      37

  Fragments arranged by size: 

                1582     271      37
 
BsrFI r'CCGG_y

Cuts at:      0     350    1675    1890 
   Size:        350    1325     215

  Fragments arranged by size: 

                1325     350     215
 
BsrGI T'GTAC_A

Cuts at:      0    1400    1890 
   Size:       1400     490
 
BssSI C'ACGA_G

Cuts at:      0     950     973    1890 
   Size:        950      23     917

  Fragments arranged by size: 

                 950     917      23
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1038    1068    1283    1890 
   Size:       1038      30     215     607

  Fragments arranged by size: 

                1038     607     215      30
 
BstYI r'GATC_y

Cuts at:      0     372     390     458    1890 
   Size:        372      18      68    1432

  Fragments arranged by size: 

                1432     372      68      18
 
Bsu36I CC'TnA_GG

Cuts at:      0    1785    1890 
   Size:       1785     105
 
BtgI C'CryG_G

Cuts at:      0     381     557    1327    1367    1589    1890 
   Size:        381     176     770      40     222     301

  Fragments arranged by size: 

                 770     381     301     222     176      40
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1563    1890 
   Size:       1563     327
 
BtsI GCAGTG_nn'

Cuts at:      0     484     684    1281    1890 
   Size:        484     200     597     609

  Fragments arranged by size: 

                 609     597     484     200
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1781    1816    1890 
   Size:       1781      35      74

  Fragments arranged by size: 

                1781      74      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1849    1890 
   Size:       1849      41
 
EaeI y'GGCC_r

Cuts at:      0      12    1297    1370    1890 
   Size:         12    1285      73     520

  Fragments arranged by size: 

                1285     520      73      12
 
EagI C'GGCC_G

Cuts at:      0      12    1890 
   Size:         12    1878
 
EarI CTCTTCn'nnn_

Cuts at:      0     160     280     536     716    1004    1205    1353    1772 
   Size:        160     120     256     180     288     201     148     419

Cuts at:   1772    1890 
   Size:        118

  Fragments arranged by size: 

                 419     288     256     201     180     160     148     120
                 118
 
EcoICRI GAG'CTC

Cuts at:      0     714     869    1254    1550    1890 
   Size:        714     155     385     296     340

  Fragments arranged by size: 

                 714     385     340     296     155
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141     346     469     484     496     574     757     862 
   Size:        141     205     123      15      12      78     183     105

Cuts at:    862    1024    1108    1120    1435    1706    1890 
   Size:        162      84      12     315     271     184

  Fragments arranged by size: 

                 315     271     205     184     183     162     141     123
                 105      84      78      15      12      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     328     739    1090    1890 
   Size:        328     411     351     800

  Fragments arranged by size: 

                 800     411     351     328
 
EcoO109I rG'GnC_Cy

Cuts at:      0      78     744    1890 
   Size:         78     666    1146

  Fragments arranged by size: 

                1146     666      78
 
HaeII r_GCGC'y

Cuts at:      0     186     324     656    1890 
   Size:        186     138     332    1234

  Fragments arranged by size: 

                1234     332     186     138
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     159     191    1534    1566    1729    1761    1813    1845 
   Size:        159      32    1343      32     163      32      52      32

Cuts at:   1845    1890 
   Size:         45

  Fragments arranged by size: 

                1343     163     159      52      45      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1827    1890 
   Size:       1827      63
 
KasI G'GCGC_C

Cuts at:      0     320     652    1890 
   Size:        320     332    1238

  Fragments arranged by size: 

                1238     332     320
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1865    1890 
   Size:       1865      25
 
MspA1I CmG'CkG

Cuts at:      0     341     383    1035    1329    1369    1890 
   Size:        341      42     652     294      40     521

  Fragments arranged by size: 

                 652     521     341     294      42      40
 
NarI GG'CG_CC

Cuts at:      0     321     653    1890 
   Size:        321     332    1237

  Fragments arranged by size: 

                1237     332     321
 
NcoI C'CATG_G

Cuts at:      0     557    1890 
   Size:        557    1333
 
NspI r_CATG'y

Cuts at:      0     109    1890 
   Size:        109    1781
 
PasI CC'CwG_GG

Cuts at:      0     208     633    1890 
   Size:        208     425    1257

  Fragments arranged by size: 

                1257     425     208
 
PfoI T'CCnGG_A

Cuts at:      0     368     697     707    1890 
   Size:        368     329      10    1183

  Fragments arranged by size: 

                1183     368     329      10
 
PmlI CAC'GTG

Cuts at:      0     279    1890 
   Size:        279    1611
 
PpuMI rG'GwC_Cy

Cuts at:      0     744    1890 
   Size:        744    1146
 
PshAI GACnn'nnGTC

Cuts at:      0     118     217    1890 
   Size:        118      99    1673

  Fragments arranged by size: 

                1673     118      99
 
PstI C_TGCA'G

Cuts at:      0    1028    1043    1073    1385    1890 
   Size:       1028      15      30     312     505

  Fragments arranged by size: 

                1028     505     312      30      15
 
PvuII CAG'CTG

Cuts at:      0    1035    1890 
   Size:       1035     855
 
RsrII CG'GwC_CG

Cuts at:      0    1016    1890 
   Size:       1016     874
 
SacI G_AGCT'C

Cuts at:      0     716     871    1256    1552    1890 
   Size:        716     155     385     296     338

  Fragments arranged by size: 

                 716     385     338     296     155
 
SacII CC_GC'GG

Cuts at:      0     384    1330    1370    1890 
   Size:        384     946      40     520

  Fragments arranged by size: 

                 946     520     384      40
 
SapI GCTCTTCn'nnn_

Cuts at:      0     280    1004    1772    1890 
   Size:        280     724     768     118

  Fragments arranged by size: 

                 768     724     280     118
 
SbfI CC_TGCA'GG

Cuts at:      0    1073    1385    1890 
   Size:       1073     312     505

  Fragments arranged by size: 

                1073     505     312
 
SfcI C'TryA_G

Cuts at:      0     222    1024    1039    1069    1381    1890 
   Size:        222     802      15      30     312     509

  Fragments arranged by size: 

                 802     509     312     222      30      15
 
SfoI GGC'GCC

Cuts at:      0     322     654    1890 
   Size:        322     332    1236

  Fragments arranged by size: 

                1236     332     322
 
SmaI CCC'GGG

Cuts at:      0    1657    1890 
   Size:       1657     233
 
SphI G_CATG'C

Cuts at:      0     109    1890 
   Size:        109    1781
 
SrfI GCCC'GGGC

Cuts at:      0    1657    1890 
   Size:       1657     233
 
StuI AGG'CCT

Cuts at:      0    1535    1798    1890 
   Size:       1535     263      92

  Fragments arranged by size: 

                1535     263      92
 
StyI C'CwwG_G

Cuts at:      0     557    1799    1890 
   Size:        557    1242      91

  Fragments arranged by size: 

                1242     557      91
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     532    1536    1890 
   Size:        532    1004     354

  Fragments arranged by size: 

                1004     532     354
 
TatI w'GTAC_w

Cuts at:      0    1400    1890 
   Size:       1400     490
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      94     126     463     495    1087    1119    1890 
   Size:         94      32     337      32     592      32     771

  Fragments arranged by size: 

                 771     592     337      94      32      32      32
 
XmaI C'CCGG_G

Cuts at:      0    1655    1890 
   Size:       1655     235
 
ZraI GAC'GTC

Cuts at:      0    1824    1890 
   Size:       1824      66
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AgeI     AlfI    AlwNI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BlpI Bme1580I    BmgBI
     BmrI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI    CspCI     DrdI     EaeI     EagI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I   HincII     KasI
     MmeI   MspA1I     NarI     NcoI     NspI     PasI     PfoI     PmlI
    PpuMI    PshAI     PstI    PvuII    RsrII     SacI    SacII     SapI
     SbfI     SfcI     SfoI     SmaI     SphI     SrfI     StuI     StyI
    TaqII     TatI     TstI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII   AflIII     AhdI     AleI
     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BmtI
     BplI    BpuEI    BsiWI     BsmI    BspEI    BspHI    BspMI   BssHII
    BstBI   BstEII    BstXI  BstZ17I     ClaI     DraI   DraIII     EciI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI
     KpnI     MfeI     MluI     MscI     MslI     NaeI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI
     PpiI     PsiI   PspOMI    PspXI     PsrI     PvuI     SalI    SanDI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SspI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmnI
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