Restriction map for pF1KB8996
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvrII       BalI        BbrPI       
BbsI        BbvCI       BciVI       BclI        BfrI        
BglII       BlnI        BpuAI       BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     ClaI        CpoI        DraI        DrdI        
Eam1105I    Eco47III    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        HincII      HindII      HindIII     
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AviII         320  TGCGCA               TGC^GCA
BamHI         136  GGATCC               G^GATCC
BbeI          397  GGCGCC               GGCGC^C
BcgI          858  GCACCGCATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          742  CTAG                 C^TAG
BlpI         1128  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1128  GCTGAGC              GC^T[ACGT]AGC
BsmAI         442  GAGAC                GTCTC(1/5)
BstAPI        285  GCACGCCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        702  CATCC                GGATG(2/0)
CelII        1128  GCTGAGC              GC^T[ACGT]AGC
DraIII        286  CACGCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI         564  CCTGGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       305  CCATGG               C^C[AT][AT]GG
FokI          702  CATCC                GGATG(9/13)
FseI          268  GGCCGGCC             GGCCGG^CC
FspI          320  TGCGCA               TGC^GCA
HgaI          102  GACGC                GACGC(5/10)
KasI          397  GGCGCC               G^GCGCC
MaeI          742  CTAG                 C^TAG
NarI          397  GGCGCC               GG^CGCC
NcoI          305  CCATGG               C^CATGG
NspI         1076  GCATGC               [GA]CATG^[CT]
PpuMI        1025  GGGTCCC              [GA]G^G[AT]CC[CT]
PstI          256  CTGCAG               CTGCA^G
SacI          196  GAGCTC               GAGCT^C
SfaNI         261  GATGC                GCATC(5/9)
SfcI          256  CTGCAG               C^T[GA][CT]AG
SfiI          699  GGCCATCCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          397  GGCGCC               GGC^GCC
SphI         1076  GCATGC               GCATG^C
StyI          305  CCATGG               C^C[AT][AT]GG
TspRI         751  CAGTG                CA[GC]TG[ACGT][ACGT]^
XcmI         1190  CCAACATGATTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcyI          102  GACGCC               G[GA]^CG[CT]C
              397  GGCGCC              
AocI           70  CCTGAGG              CC^T[ACGT]AGG
              295  CCTAAGG             
AspHI         196  GAGCTC               G[AT]GC[AT]^C
             1131  GAGCAC              
BanII          81  GGGCTC               G[GA]GC[CT]^C
              196  GAGCTC              
BsaHI         102  GACGCC               G[GA]^CG[CT]C
              397  GGCGCC              
BsiHKAI       196  GAGCTC               G[AT]GC[AT]^C
             1131  GAGCAC              
BsmFI        1181  GGGAC                GGGAC(10/14)
             1027  GTCCC               
BsmI          863  GCATTC               GAATGC(1/-1)
             1115  GCATTC              
BsrBI         718  GAGCGG               CCGCTC(-3/-3)
              727  GAGCGG              
BsrI          956  ACTGG                ACTGG(1/-1)
             1068  CCAGT               
BstYI         136  GGATCC               [GA]^GATC[CT]
              365  AGATCC              
Bsu36I         70  CCTGAGG              CC^T[ACGT]AGG
              295  CCTAAGG             
DraII          66  GGGGCCT              [GA]G^G[ACGT]CC[CT]
             1025  GGGTCCC             
EarI          648  GAAGAG               CTCTTC(1/4)
              805  GAAGAG              
Eco81I         70  CCTGAGG              CC^T[ACGT]AGG
              295  CCTAAGG             
EcoO109I       66  GGGGCCT              [GA]G^G[ACGT]CC[CT]
             1025  GGGTCCC             
HaeII          21  GGCGCT               [GA]GCGC^[CT]
              397  GGCGCC              
Hin1I         102  GACGCC               G[GA]^CG[CT]C
              397  GGCGCC              
HinfI         734  GACTC                G^A[ACGT]TC
              808  GAGTC               
Ksp632I       648  GAAGAG               CTCTTC(1/4)
              805  GAAGAG              
MflI          136  GGATCC               [GA]^GATC[CT]
              365  AGATCC              
PflMI         527  CCAGCCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              765  CCAGGAGCTGG         
PleI          734  GACTC                GAGTC(4/5)
              808  GAGTC               
PvuII         759  CAGCTG               CAG^CTG
             1002  CAGCTG              
SmlI          246  CTTGAG               C^T[CT][GA]AG
              661  CTCAAG              
TaqI          377  TCGA                 T^CGA
             1154  TCGA                
TthHB8I       377  TCGA                 T^CGA
             1154  TCGA                
Van91I        527  CCAGCCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              765  CCAGGAGCTGG         
XhoII         136  GGATCC               [GA]^GATC[CT]
              365  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AvaI        AvaII       BanI        BbvI        
BcnI        BglI        BmyI        BpmI        BsaJI       
BseRI       BsgI        BsiEI       BsiYI       BslI        
BsoBI       Bsp1286I    BsrFI       BssKI       BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DsaI        EaeI        EagI        
EclXI       Eco52I      EcoRII      FauI        Fnu4HI      
HaeIII      HapII       HhaI        HinP1I      HpaII       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       RsaI        
Sau3AI      Sau96I      ScrFI       SmaI        StuI        
TseI        Tsp45I      XmaCI       XmaI        
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