Restriction map for
pF1KB8996
(Circular) (Six-base) MAPSORT of: pF1KB8996.seq Check: 2891 from: 1 to: 4334
pF1KB8996 4334 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 1372 1372
Size: 4334
Acc65I G'GTAC_C
Cuts at: 1350 1350
Size: 4334
AclI AA'CG_TT
Cuts at: 4174 4174
Size: 4334
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2159 2591 3338 3606 2159
Size: 432 747 268 2887
Fragments arranged by size:
2887 747 432 268
AflIII A'CryG_T
Cuts at: 1686 2790 3767 1686
Size: 1104 977 2253
Fragments arranged by size:
2253 1104 977
AgeI A'CCGG_T
Cuts at: 2722 2722
Size: 4334
AleI CACnn'nnGTG
Cuts at: 1165 1165
Size: 4334
AlwNI CAG_nnn'CTG
Cuts at: 618 782 879 1639 3206 618
Size: 164 97 760 1567 1746
Fragments arranged by size:
1746 1567 760 164 97
ApaLI G'TGCA_C
Cuts at: 3104 3104
Size: 4334
ApoI r'AATT_y
Cuts at: 1338 3687 3943 1338
Size: 2349 256 1729
Fragments arranged by size:
2349 1729 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4334
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
AvaI C'yCGr_G
Cuts at: 249 501 572 1354 249
Size: 252 71 782 3229
Fragments arranged by size:
3229 782 252 71
BamHI G'GATC_C
Cuts at: 244 1359 244
Size: 1115 3219
BanI G'GyrC_C
Cuts at: 505 888 965 1350 1764 2012 2047 505
Size: 383 77 385 414 248 35 2792
Fragments arranged by size:
2792 414 385 383 248 77 35
BanII G_rGCy'C
Cuts at: 193 308 1348 2378 193
Size: 115 1040 1030 2149
Fragments arranged by size:
2149 1040 1030 115
BbeI G_GCGC'C
Cuts at: 509 1768 2016 509
Size: 1259 248 2827
Fragments arranged by size:
2827 1259 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 955 989 955
Size: 34 4300
BciVI GTATCCnnnnn_n'
Cuts at: 2232 2993 4334 2232
Size: 761 1341 2232
Fragments arranged by size:
2232 1341 761
BclI T'GATC_A
Cuts at: 1854 1854
Size: 4334
BglI GCCn_nnn'nGGC
Cuts at: 814 1052 1126 814
Size: 238 74 4022
Fragments arranged by size:
4022 238 74
BglII A'GATC_T
Cuts at: 1849 3886 1849
Size: 2037 2297
BlpI GC'TnA_GC
Cuts at: 1237 1446 1237
Size: 209 4125
Bme1580I G_kGCm'C
Cuts at: 1298 1959 2052 3108 1298
Size: 661 93 1056 2524
Fragments arranged by size:
2524 1056 661 93
BmgBI CAC'GTC
Cuts at: 855 1293 855
Size: 438 3896
BmrI ACTGGGnnnn_n'
Cuts at: 1546 1627 1962 4106 1546
Size: 81 335 2144 1774
Fragments arranged by size:
2144 1774 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2701 2701
Size: 4334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 694 1009 352
Size: 342 315 3677
Fragments arranged by size:
3677 342 315
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 375 754 1510 2389 2881 3179 375
Size: 379 756 879 492 298 1530
Fragments arranged by size:
1530 879 756 492 379 298
BsaI GGTCTCn'nnnn_
Cuts at: 4038 4038
Size: 4334
BsaAI yAC'GTr
Cuts at: 1687 1687
Size: 4334
BsaBI GATnn'nnATC
Cuts at: 6 1848 3885 6
Size: 1842 2037 455
Fragments arranged by size:
2037 1842 455
BsaHI Gr'CG_yC
Cuts at: 211 506 1765 2013 2715 4211 211
Size: 295 1259 248 702 1496 334
Fragments arranged by size:
1496 1259 702 334 295 248
BsaWI w'CCGG_w
Cuts at: 1523 2044 2722 2996 3143 3554 1523
Size: 521 678 274 147 411 2303
Fragments arranged by size:
2303 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 316 558 1140 1327 316
Size: 242 582 187 3323
Fragments arranged by size:
3323 582 242 187
BseYI C'CCAG_C
Cuts at: 132 171 951 1116 1759 2517 3094 3590 132
Size: 39 780 165 643 758 577 496 876
Fragments arranged by size:
876 780 758 643 577 496 165 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 877 961 421
Size: 456 84 3794
Fragments arranged by size:
3794 456 84
BsiEI CG_ry'CG
Cuts at: 104 126 164 312 465 1876 1922 3130 104
Size: 22 38 148 153 1411 46 1208 1308
Fragments arranged by size:
1411 1308 1208 153 148 46 38 22
BsiHKAI G_wGCw'C
Cuts at: 308 1243 1348 2126 2316 3108 308
Size: 935 105 778 190 792 1534
Fragments arranged by size:
1534 935 792 778 190 105
BsmI GAATG_Cn'
Cuts at: 971 1223 971
Size: 252 4082
Bsp1286I G_dGCh'C
Cuts at: 193 308 1243 1298 1348 1959 2052 2126 2316
Size: 115 935 55 50 611 93 74 190
Cuts at: 2316 2378 3108 193
Size: 62 730 1419
Fragments arranged by size:
1419 935 730 611 190 115 93 74
62 55 50
BspEI T'CCGG_A
Cuts at: 3554 3554
Size: 4334
BspHI T'CATG_A
Cuts at: 3510 3510
Size: 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1384 1598 1900 2281 1384
Size: 214 302 381 3437
Fragments arranged by size:
3437 381 302 214
BsrBI CCG'CTC
Cuts at: 50 828 837 2626 2680 4164 4329 50
Size: 778 9 1789 54 1484 165 55
Fragments arranged by size:
1789 1484 778 165 55 54 9
BsrDI GCAATG_nn'
Cuts at: 2246 2246
Size: 4334
BsrFI r'CCGG_y
Cuts at: 163 377 686 891 923 1081 2332 2513 2722
Size: 214 309 205 32 158 1251 181 209
Cuts at: 2722 163
Size: 1775
Fragments arranged by size:
1775 1251 309 214 209 205 181 158
32
BssSI C'ACGA_G
Cuts at: 2605 2963 2605
Size: 358 3976
BstAPI GCAn_nnn'nTGC
Cuts at: 399 1711 399
Size: 1312 3022
BstBI TT'CG_AA
Cuts at: 2695 2695
Size: 4334
BstYI r'GATC_y
Cuts at: 1 244 473 1359 1826 1849 2184 2430 3442
Size: 243 229 886 467 23 335 246 1012
Cuts at: 3442 3528 3540 3886 1
Size: 86 12 346 449
Fragments arranged by size:
1012 886 467 449 346 335 246 243
229 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 179 404 179
Size: 225 4109
BtgI C'CryG_G
Cuts at: 107 413 729 884 107
Size: 306 316 155 3557
Fragments arranged by size:
3557 316 306 155
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1010 2284 2465 2628 1010
Size: 1274 181 163 2716
Fragments arranged by size:
2716 1274 181 163
BtsI GCAGTG_nn'
Cuts at: 865 1577 865
Size: 712 3622
DraI TTT'AAA
Cuts at: 1333 1333
Size: 4334
DraIII CAC_nnn'GTG
Cuts at: 399 399
Size: 4334
DrdI GACnn_nn'nnGTC
Cuts at: 2040 2898 2040
Size: 858 3476
EaeI y'GGCC_r
Cuts at: 161 295 309 462 489 513 881 999 1919
Size: 134 14 153 27 24 368 118 920
Cuts at: 1919 2093 2484 2511 3856 161
Size: 174 391 27 1345 639
Fragments arranged by size:
1345 920 639 391 368 174 153 134
118 27 27 24 14
EagI C'GGCC_G
Cuts at: 161 309 462 1919 161
Size: 148 153 1457 2576
Fragments arranged by size:
2576 1457 153 148
EarI CTCTTCn'nnn_
Cuts at: 751 908 2357 2567 751
Size: 157 1449 210 2518
Fragments arranged by size:
2518 1449 210 157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 568 705 1033 2542 2852 2998 4144 568
Size: 137 328 1509 310 146 1146 758
Fragments arranged by size:
1509 1146 758 328 310 146 137
EcoICRI GAG'CTC
Cuts at: 306 1346 306
Size: 1040 3294
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 694 1009 2159 2591 3338 3606 352
Size: 342 315 1150 432 747 268 1080
Fragments arranged by size:
1150 1080 747 432 342 315 268
EcoNI CCTnn'n_nnAGG
Cuts at: 676 676
Size: 4334
EcoO109I rG'GnC_Cy
Cuts at: 175 1134 1473 175
Size: 959 339 3036
Fragments arranged by size:
3036 959 339
EcoRI G'AATT_C
Cuts at: 1338 1338
Size: 4334
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2353 2353
Size: 4334
FseI GG_CCGG'CC
Cuts at: 381 381
Size: 4334
FspI TGC'GCA
Cuts at: 430 2115 430
Size: 1685 2649
HaeII r_GCGC'y
Cuts at: 133 509 1768 2016 3038 4017 133
Size: 376 1259 248 1022 979 450
Fragments arranged by size:
1259 1022 979 450 376 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2176 2206 2208 2238 2176
Size: 30 2 30 4272
Fragments arranged by size:
4272 30 30 2
HincII GTy'rAC
Cuts at: 1373 1373
Size: 4334
KasI G'GCGC_C
Cuts at: 505 1764 2012 505
Size: 1259 248 2827
Fragments arranged by size:
2827 1259 248
KpnI G_GTAC'C
Cuts at: 1354 1354
Size: 4334
MluI A'CGCG_T
Cuts at: 3767 3767
Size: 4334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 623 3005 3189 623
Size: 2382 184 1768
Fragments arranged by size:
2382 1768 184
MscI TGG'CCA
Cuts at: 2095 2095
Size: 4334
MslI CAynn'nnrTG
Cuts at: 1165 1249 1306 2323 2450 1165
Size: 84 57 1017 127 3049
Fragments arranged by size:
3049 1017 127 84 57
MspA1I CmG'CkG
Cuts at: 779 869 1112 1445 1759 2119 3132 3377 779
Size: 90 243 333 314 360 1013 245 1736
Fragments arranged by size:
1736 1013 360 333 314 245 243 90
NaeI GCC'GGC
Cuts at: 165 379 688 2515 165
Size: 214 309 1827 1984
Fragments arranged by size:
1984 1827 309 214
NarI GG'CG_CC
Cuts at: 506 1765 2013 506
Size: 1259 248 2827
Fragments arranged by size:
2827 1259 248
NcoI C'CATG_G
Cuts at: 107 413 107
Size: 306 4028
NgoMIV G'CCGG_C
Cuts at: 163 377 686 2513 163
Size: 214 309 1827 1984
Fragments arranged by size:
1984 1827 309 214
NspI r_CATG'y
Cuts at: 1188 1387 1887 2794 1188
Size: 199 500 907 2728
Fragments arranged by size:
2728 907 500 199
PciI A'CATG_T
Cuts at: 2790 2790
Size: 4334
PflMI CCAn_nnn'nTGG
Cuts at: 641 879 641
Size: 238 4096
PfoI T'CCnGG_A
Cuts at: 329 671 716 329
Size: 342 45 3947
Fragments arranged by size:
3947 342 45
PmeI GTTT'AAAC
Cuts at: 1333 1333
Size: 4334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3505 3473
Size: 32 4302
PpuMI rG'GwC_Cy
Cuts at: 1134 1134
Size: 4334
PstI C_TGCA'G
Cuts at: 368 1381 2066 368
Size: 1013 685 2636
Fragments arranged by size:
2636 1013 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4334
PvuII CAG'CTG
Cuts at: 869 1112 1759 2119 869
Size: 243 647 360 3084
Fragments arranged by size:
3084 647 360 243
RsrII CG'GwC_CG
Cuts at: 2529 2529
Size: 4334
SacI G_AGCT'C
Cuts at: 308 1348 308
Size: 1040 3294
SalI G'TCGA_C
Cuts at: 1371 1371
Size: 4334
SanDI GG'GwC_CC
Cuts at: 1134 1134
Size: 4334
SapI GCTCTTCn'nnn_
Cuts at: 2357 2567 2357
Size: 210 4124
SbfI CC_TGCA'GG
Cuts at: 1381 1381
Size: 4334
ScaI AGT'ACT
Cuts at: 3851 3851
Size: 4334
SfcI C'TryA_G
Cuts at: 33 364 1377 2062 3055 3246 33
Size: 331 1013 685 993 191 1121
Fragments arranged by size:
1121 1013 993 685 331 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 814 814
Size: 4334
SfoI GGC'GCC
Cuts at: 507 1766 2014 507
Size: 1259 248 2827
Fragments arranged by size:
2827 1259 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
SmaI CCC'GGG
Cuts at: 251 503 574 1356 251
Size: 252 71 782 3229
Fragments arranged by size:
3229 782 252 71
SmlI C'TyrA_G
Cuts at: 354 769 1489 2404 2896 3158 354
Size: 415 720 915 492 262 1530
Fragments arranged by size:
1530 915 720 492 415 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4334
SphI G_CATG'C
Cuts at: 1188 1387 1887 1188
Size: 199 500 3635
Fragments arranged by size:
3635 500 199
SrfI GCCC'GGGC
Cuts at: 503 503
Size: 4334
StuI AGG'CCT
Cuts at: 184 817 986 184
Size: 633 169 3532
Fragments arranged by size:
3532 633 169
StyI C'CwwG_G
Cuts at: 107 413 1468 1822 107
Size: 306 1055 354 2619
Fragments arranged by size:
2619 1055 354 306
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 210 1092 1917 2050 2509 2718 210
Size: 882 825 133 459 209 1826
Fragments arranged by size:
1826 882 825 459 209 133
TatI w'GTAC_w
Cuts at: 710 1256 1271 3849 710
Size: 546 15 2578 1195
Fragments arranged by size:
2578 1195 546 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1086 1118 3851 3883 1086
Size: 32 2733 32 1537
Fragments arranged by size:
2733 1537 32 32
Tth111I GACn'n_nGTC
Cuts at: 2131 2131
Size: 4334
XbaI T'CTAG_A
Cuts at: 1365 1365
Size: 4334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1305 1829 1305
Size: 524 3810
XmaI C'CCGG_G
Cuts at: 249 501 572 1354 249
Size: 252 71 782 3229
Fragments arranged by size:
3229 782 252 71
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmtI Bpu10I
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI EcoRV FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SexAI SgrAI SnaBI
SspI SwaI XhoI XmnI ZraI