Restriction map for pF1KB3719
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaLI       AscI        AseI        AsnI        
AspEI       AviII       AvrII       BamHI       BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsiEI       BsiWI       BsmI        
Bsp1407I    BspDI       BspLU11I    BsrDI       BsrGI       
BssSI       Bst1107I    BstZ17I     CelII       ClaI        
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoRI       EcoRV       FbaI        
FseI        FspI        HindIII     HpaI        MamI        
MfeI        MluI        MunI        NdeI        NotI        
NruI        PacI        PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       PvuI        RsrII       
SalI        SbfI        ScaI        SexAI       SgrAI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
XbaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AatII        1534  GACGTC               GACGT^C
Acc65I       1565  GGTACC               G^GTACC
AccI         2306  GTAGAC               GT^[AC][GT]AC
AccIII        939  TCCGGA               T^CCGGA
AclI          368  AACGTT               AA^CGTT
AocI         2505  CCTGAGG              CC^T[ACGT]AGG
Asp700I       931  GAACCAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1565  GGTACC               G^GTACC
AspI         1177  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1898  TGGCCA               TGG^CCA
BfaI         1039  CTAG                 C^TAG
BsaWI         939  TCCGGA               [AT]^CCGG[AT]
BseAI         939  TCCGGA               T^CCGGA
BsmBI        2204  CGTCTC               CGTCTC(1/5)
BspEI         939  TCCGGA               T^CCGGA
BspHI        1274  TCATGA               T^CATGA
BsrBI        1570  CCGCTC               CCGCTC(-3/-3)
BssHII       1353  GCGCGC               G^CGCGC
BstAPI       2361  GCAGTCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1753  TTCGAA               TT^CGAA
BstXI         986  CCATCCTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       2505  CCTGAGG              CC^T[ACGT]AGG
Eco81I       2505  CCTGAGG              CC^T[ACGT]AGG
EcoNI        2217  CCTTCCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       585  ATGCAT               ATGCA^T
HincII        451  GTTGAC               GT[CT]^[GA]AC
HindII        451  GTTGAC               GT[CT]^[GA]AC
KpnI         1565  GGTACC               GGTAC^C
MaeI         1039  CTAG                 C^TAG
MluNI        1898  TGGCCA               TGG^CCA
MroI          939  TCCGGA               T^CCGGA
MscI         1898  TGGCCA               TGG^CCA
MseI          818  TTAA                 T^TAA
NaeI         2469  GCCGGC               GCC^GGC
NgoMIV       2469  GCCGGC               G^CCGGC
NheI         1038  GCTAGC               G^CTAGC
NsiI          585  ATGCAT               ATGCA^T
NspV         1753  TTCGAA               TT^CGAA
PaeR7I        325  CTCGAG               C^TCGAG
PflFI        1177  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I      368  AACGTT               AA^CGTT
RcaI         1274  TCATGA               T^CATGA
SfuI         1753  TTCGAA               TT^CGAA
SmlI          325  CTCGAG               C^T[CT][GA]AG
SnaBI        1495  TACGTA               TAC^GTA
SphI         1637  GCATGC               GCATG^C
Tru9I         818  TTAA                 T^TAA
Tth111I      1177  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          325  CTCGAG               C^TCGAG
XmnI          931  GAACCAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AcsI          534  GAATTT               [GA]^AATT[CT]
             1189  AAATTC              
ApoI          534  GAATTT               [GA]^AATT[CT]
             1189  AAATTC              
BsaAI        1171  TACGTG               [CT]AC^GT[GA]
             1495  TACGTA              
BsaI          263  GAGACC               GGTCTC(1/5)
              628  GAGACC              
BsrFI        1843  ACCGGC               [GA]^CCGG[CT]
             2469  GCCGGC              
BstEII        474  GGTGACC              G^GT[ACGT]ACC
              573  GGTAACC             
BstPI         474  GGTGACC              G^GT[ACGT]ACC
              573  GGTAACC             
Cfr10I       1843  ACCGGC               [GA]^CCGG[CT]
             2469  GCCGGC              
EaeI         1898  TGGCCA               [CT]^GGCC[GA]
             2033  TGGCCG              
EcoO65I       474  GGTGACC              G^GT[ACGT]ACC
              573  GGTAACC             
NcoI         2198  CCATGG               C^CATGG
             2315  CCATGG              
SacI          591  GAGCTC               GAGCT^C
             2397  GAGCTC              
SapI         1246  GAAGAGC              GCTCTTC(1/4)
             2262  GCTCTTC             
SfiI         1890  GGCCTGCCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             2025  GGCCCTGGTGGCC       
StuI         1889  AGGCCT               AGG^CCT
             2102  AGGCCT              
TfiI          186  GATTC                G^A[AT]TC
              676  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BanI        BanII       BbeI        BbvI        
BcnI        BglI        BmyI        BpmI        BsaHI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DraIII      DrdI        
DsaI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     KspI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NarI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SacII       Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SfoI        SmaI        StyI        
TaqI        TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII       XmaCI       
XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com