Restriction map for pF1KB3719
 (Linear) (Six-base) MAPSORT of: KIBB3719.seq  Check: 7075  from: 1  to: 2598



KIBB3719.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     497    1967    2598 
   Size:        497    1470     631

  Fragments arranged by size: 

                1470     631     497
 
AatII G_ACGT'C

Cuts at:      0    1538    2598 
   Size:       1538    1060
 
AccI GT'mk_AC

Cuts at:      0    2307    2598 
   Size:       2307     291
 
Acc65I G'GTAC_C

Cuts at:      0    1565    2598 
   Size:       1565    1033
 
AclI AA'CG_TT

Cuts at:      0     369    2598 
   Size:        369    2229
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     420     843    1144    1494    2598 
   Size:        420     423     301     350    1104

  Fragments arranged by size: 

                1104     423     420     350     301
 
AleI CACnn'nnGTG

Cuts at:      0    1225    2002    2598 
   Size:       1225     777     596

  Fragments arranged by size: 

                1225     777     596
 
AlwNI CAG_nnn'CTG

Cuts at:      0     696     866    1203    1614    2277    2598 
   Size:        696     170     337     411     663     321

  Fragments arranged by size: 

                 696     663     411     337     321     170
 
ApaI G_GGCC'C

Cuts at:      0      41    2028    2054    2591    2598 
   Size:         41    1987      26     537       7

  Fragments arranged by size: 

                1987     537      41      26       7
 
ApoI r'AATT_y

Cuts at:      0     534    1189    2598 
   Size:        534     655    1409

  Fragments arranged by size: 

                1409     655     534
 
AvaI C'yCGr_G

Cuts at:      0      33      81     325    1683    1967    2124    2167    2413 
   Size:         33      48     244    1358     284     157      43     246

Cuts at:   2413    2488    2553    2598 
   Size:         75      65      45

  Fragments arranged by size: 

                1358     284     246     244     157      75      65      48
                  45      43      33
 
BanI G'GyrC_C

Cuts at:      0      88    1386    1489    1557    1565    1860    2065    2598 
   Size:         88    1298     103      68       8     295     205     533

  Fragments arranged by size: 

                1298     533     295     205     103      88      68       8
            
 
BanII G_rGCy'C

Cuts at:      0      41     595    1003    1684    2028    2054    2401    2591 
   Size:         41     554     408     681     344      26     347     190

Cuts at:   2591    2598 
   Size:          7

  Fragments arranged by size: 

                 681     554     408     347     344     190      41      26
                   7
 
BbeI G_GCGC'C

Cuts at:      0      92    1390    1561    1864    2598 
   Size:         92    1298     171     303     734

  Fragments arranged by size: 

                1298     734     303     171      92
 
BfrBI ATG'CAT

Cuts at:      0     587    2598 
   Size:        587    2011
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1395    1688    1897    2032    2598 
   Size:       1395     293     209     135     566

  Fragments arranged by size: 

                1395     566     293     209     135
 
Bme1580I G_kGCm'C

Cuts at:      0      41    2028    2054    2591    2598 
   Size:         41    1987      26     537       7

  Fragments arranged by size: 

                1987     537      41      26       7
 
BmgBI CAC'GTC

Cuts at:      0    1179    2229    2598 
   Size:       1179    1050     369

  Fragments arranged by size: 

                1179    1050     369
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1191    2585    2598 
   Size:       1191    1394      13

  Fragments arranged by size: 

                1394    1191      13
 
BmtI G_CTAG'C

Cuts at:      0    1042    2598 
   Size:       1042    1556
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1335    1899    2598 
   Size:       1335     564     699

  Fragments arranged by size: 

                1335     699     564
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     655     911    1053    1636    2376    2500    2518    2598 
   Size:        655     256     142     583     740     124      18      80

  Fragments arranged by size: 

                 740     655     583     256     142     124      80      18
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     594    2598 
   Size:        594    2004
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     257     622    2598 
   Size:        257     365    1976

  Fragments arranged by size: 

                1976     365     257
 
BsaAI yAC'GTr

Cuts at:      0    1173    1497    2598 
   Size:       1173     324    1101

  Fragments arranged by size: 

                1173    1101     324
 
BsaHI Gr'CG_yC

Cuts at:      0      89     308    1387    1535    1558    1861    2203    2598 
   Size:         89     219    1079     148      23     303     342     395

  Fragments arranged by size: 

                1079     395     342     303     219     148      89      23
            
 
BsaWI w'CCGG_w

Cuts at:      0     939    2598 
   Size:        939    1659
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1922    1952    2598 
   Size:       1922      30     646

  Fragments arranged by size: 

                1922     646      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     201    1603    1834    1943    2032    2362    2365    2455 
   Size:        201    1402     231     109      89     330       3      90

Cuts at:   2455    2464    2523    2598 
   Size:          9      59      75

  Fragments arranged by size: 

                1402     330     231     201     109      90      89      75
                  59       9       3
 
BseYI C'CCAG_C

Cuts at:      0      45     131    1042    2115    2131    2558    2598 
   Size:         45      86     911    1073      16     427      40

  Fragments arranged by size: 

                1073     911     427      86      45      40      16
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     132     223     353     862    2389    2598 
   Size:        132      91     130     509    1527     209

  Fragments arranged by size: 

                1527     509     209     132     130      91
 
BsiHKAI G_wGCw'C

Cuts at:      0     560     595     743    1342    2009    2240    2401    2598 
   Size:        560      35     148     599     667     231     161     197

  Fragments arranged by size: 

                 667     599     560     231     197     161     148      35
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2210    2598 
   Size:       2210     388
 
Bsp1286I G_dGCh'C

Cuts at:      0      41     560     595     743    1003    1342    1684    2009 
   Size:         41     519      35     148     260     339     342     325

Cuts at:   2009    2028    2054    2240    2401    2591    2598 
   Size:         19      26     186     161     190       7

  Fragments arranged by size: 

                 519     342     339     325     260     190     186     161
                 148      41      35      26      19       7
 
BspEI T'CCGG_A

Cuts at:      0     939    2598 
   Size:        939    1659
 
BspHI T'CATG_A

Cuts at:      0    1274    2598 
   Size:       1274    1324
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     175     233     497     765    1247    1682    1722    1967 
   Size:        175      58     264     268     482     435      40     245

Cuts at:   1967    2598 
   Size:        631

  Fragments arranged by size: 

                 631     482     435     268     264     245     175      58
                  40
 
BsrBI CCG'CTC

Cuts at:      0    1572    2598 
   Size:       1572    1026
 
BsrFI r'CCGG_y

Cuts at:      0    1843    2469    2598 
   Size:       1843     626     129

  Fragments arranged by size: 

                1843     626     129
 
BssHII G'CGCG_C

Cuts at:      0    1353    2598 
   Size:       1353    1245
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2367    2598 
   Size:       2367     231
 
BstBI TT'CG_AA

Cuts at:      0    1754    2598 
   Size:       1754     844
 
BstEII G'GTnAC_C

Cuts at:      0     474     573    2598 
   Size:        474      99    2025

  Fragments arranged by size: 

                2025     474      99
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     993    2598 
   Size:        993    1605
 
BstYI r'GATC_y

Cuts at:      0     230     692     845    2598 
   Size:        230     462     153    1753

  Fragments arranged by size: 

                1753     462     230     153
 
Bsu36I CC'TnA_GG

Cuts at:      0    2506    2598 
   Size:       2506      92
 
BtgI C'CryG_G

Cuts at:      0     275     623    1344    1410    1421    1561    2036    2159 
   Size:        275     348     721      66      11     140     475     123

Cuts at:   2159    2198    2315    2598 
   Size:         39     117     283

  Fragments arranged by size: 

                 721     475     348     283     275     140     123     117
                  66      39      11
 
BtsI GCAGTG_nn'

Cuts at:      0     295    1436    1825    1943    2598 
   Size:        295    1141     389     118     655

  Fragments arranged by size: 

                1141     655     389     295     118
 
DraIII CAC_nnn'GTG

Cuts at:      0     447     668     840    2598 
   Size:        447     221     172    1758

  Fragments arranged by size: 

                1758     447     221     172
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1534    1732    1912    2598 
   Size:       1534     198     180     686

  Fragments arranged by size: 

                1534     686     198     180
 
EaeI y'GGCC_r

Cuts at:      0    1898    2033    2598 
   Size:       1898     135     565

  Fragments arranged by size: 

                1898     565     135
 
EarI CTCTTCn'nnn_

Cuts at:      0     767    1241    1802    2155    2269    2432    2441    2598 
   Size:        767     474     561     353     114     163       9     157

  Fragments arranged by size: 

                 767     561     474     353     163     157     114       9
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2524    2598 
   Size:       2524      74
 
EcoICRI GAG'CTC

Cuts at:      0     593    2399    2598 
   Size:        593    1806     199

  Fragments arranged by size: 

                1806     593     199
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     420     655     843     911    1053    1144    1494    1636 
   Size:        420     235     188      68     142      91     350     142

Cuts at:   1636    2376    2500    2518    2598 
   Size:        740     124      18      80

  Fragments arranged by size: 

                 740     420     350     235     188     142     142     124
                  91      80      68      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2221    2598 
   Size:       2221     377
 
EcoO109I rG'GnC_Cy

Cuts at:      0      37      38     222     513     609    1428    1611    2024 
   Size:         37       1     184     291      96     819     183     413

Cuts at:   2024    2025    2051    2457    2493    2587    2598 
   Size:          1      26     406      36      94      11

  Fragments arranged by size: 

                 819     413     406     291     184     183      96      94
                  37      36      26      11       1       1
 
HaeII r_GCGC'y

Cuts at:      0      92     121    1372    1390    1561    1864    1984    2132 
   Size:         92      29    1251      18     171     303     120     148

Cuts at:   2132    2598 
   Size:        466

  Fragments arranged by size: 

                1251     466     303     171     148     120      92      29
                  18
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     177     209     379     411     779     811    1780    1812 
   Size:        177      32     170      32     368      32     969      32

Cuts at:   1812    2598 
   Size:        786

  Fragments arranged by size: 

                 969     786     368     177     170      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     453    2598 
   Size:        453    2145
 
KasI G'GCGC_C

Cuts at:      0      88    1386    1557    1860    2598 
   Size:         88    1298     171     303     738

  Fragments arranged by size: 

                1298     738     303     171      88
 
KpnI G_GTAC'C

Cuts at:      0    1569    2598 
   Size:       1569    1029
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     815    2598 
   Size:        815    1783
 
MscI TGG'CCA

Cuts at:      0    1900    2598 
   Size:       1900     698
 
MslI CAynn'nnrTG

Cuts at:      0     622    1225    1450    2002    2197    2428    2598 
   Size:        622     603     225     552     195     231     170

  Fragments arranged by size: 

                 622     603     552     231     225     195     170
 
MspA1I CmG'CkG

Cuts at:      0      28     182     237     354     648     866    1122    1346 
   Size:         28     154      55     117     294     218     256     224

Cuts at:   1346    1523    1563    1739    1911    2038    2112    2358    2520 
   Size:        177      40     176     172     127      74     246     162

Cuts at:   2520    2526    2541    2598 
   Size:          6      15      57

  Fragments arranged by size: 

                 294     256     246     224     218     177     176     172
                 162     154     127     117      74      57      55      40
                  28      15       6
 
NaeI GCC'GGC

Cuts at:      0    2471    2598 
   Size:       2471     127
 
NarI GG'CG_CC

Cuts at:      0      89    1387    1558    1861    2598 
   Size:         89    1298     171     303     737

  Fragments arranged by size: 

                1298     737     303     171      89
 
NcoI C'CATG_G

Cuts at:      0    2198    2315    2598 
   Size:       2198     117     283

  Fragments arranged by size: 

                2198     283     117
 
NgoMIV G'CCGG_C

Cuts at:      0    2469    2598 
   Size:       2469     129
 
NheI G'CTAG_C

Cuts at:      0    1038    2598 
   Size:       1038    1560
 
NsiI A_TGCA'T

Cuts at:      0     589    2598 
   Size:        589    2009
 
NspI r_CATG'y

Cuts at:      0     747     951    1641    2598 
   Size:        747     204     690     957

  Fragments arranged by size: 

                 957     747     690     204
 
PasI CC'CwG_GG

Cuts at:      0    2292    2461    2598 
   Size:       2292     169     137

  Fragments arranged by size: 

                2292     169     137
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     447    1614    2001    2598 
   Size:        447    1167     387     597

  Fragments arranged by size: 

                1167     597     447     387
 
PfoI T'CCnGG_A

Cuts at:      0     104    1460    1607    1721    1836    2392    2598 
   Size:        104    1356     147     114     115     556     206

  Fragments arranged by size: 

                1356     556     206     147     115     114     104
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1756    1788    2598 
   Size:       1756      32     810

  Fragments arranged by size: 

                1756     810      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     222     513     609    1428    1611    2457    2493    2598 
   Size:        222     291      96     819     183     846      36     105

  Fragments arranged by size: 

                 846     819     291     222     183     105      96      36
            
 
PspOMI G'GGCC_C

Cuts at:      0      37    2024    2050    2587    2598 
   Size:         37    1987      26     537      11

  Fragments arranged by size: 

                1987     537      37      26      11
 
PspXI vC'TCGA_Gb

Cuts at:      0     325    2598 
   Size:        325    2273
 
PstI C_TGCA'G

Cuts at:      0     230     302     353    1443    1964    2305    2546    2598 
   Size:        230      72      51    1090     521     341     241      52

  Fragments arranged by size: 

                1090     521     341     241     230      72      52      51
            
 
PvuII CAG'CTG

Cuts at:      0     182     237     354     648     866    1523    2526    2541 
   Size:        182      55     117     294     218     657    1003      15

Cuts at:   2541    2598 
   Size:         57

  Fragments arranged by size: 

                1003     657     294     218     182     117      57      55
                  15
 
SacI G_AGCT'C

Cuts at:      0     595    2401    2598 
   Size:        595    1806     197

  Fragments arranged by size: 

                1806     595     197
 
SacII CC_GC'GG

Cuts at:      0    1347    1564    2039    2598 
   Size:       1347     217     475     559

  Fragments arranged by size: 

                1347     559     475     217
 
SanDI GG'GwC_CC

Cuts at:      0     513     609    2598 
   Size:        513      96    1989

  Fragments arranged by size: 

                1989     513      96
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1241    2269    2598 
   Size:       1241    1028     329

  Fragments arranged by size: 

                1241    1028     329
 
SfcI C'TryA_G

Cuts at:      0     226     298     349    1439    1960    2301    2542    2598 
   Size:        226      72      51    1090     521     341     241      56

  Fragments arranged by size: 

                1090     521     341     241     226      72      56      51
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1897    2032    2598 
   Size:       1897     135     566

  Fragments arranged by size: 

                1897     566     135
 
SfoI GGC'GCC

Cuts at:      0      90    1388    1559    1862    2598 
   Size:         90    1298     171     303     736

  Fragments arranged by size: 

                1298     736     303     171      90
 
SmaI CCC'GGG

Cuts at:      0      35      83    1685    1969    2126    2598 
   Size:         35      48    1602     284     157     472

  Fragments arranged by size: 

                1602     472     284     157      48      35
 
SmlI C'TyrA_G

Cuts at:      0     325    2598 
   Size:        325    2273
 
SnaBI TAC'GTA

Cuts at:      0    1497    2598 
   Size:       1497    1101
 
SphI G_CATG'C

Cuts at:      0    1641    2598 
   Size:       1641     957
 
SrfI GCCC'GGGC

Cuts at:      0    1685    2126    2598 
   Size:       1685     441     472

  Fragments arranged by size: 

                1685     472     441
 
StuI AGG'CCT

Cuts at:      0    1891    2104    2598 
   Size:       1891     213     494

  Fragments arranged by size: 

                1891     494     213
 
StyI C'CwwG_G

Cuts at:      0    2181    2198    2315    2598 
   Size:       2181      17     117     283

  Fragments arranged by size: 

                2181     283     117      17
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     268     948    1272    2598 
   Size:        268     680     324    1326

  Fragments arranged by size: 

                1326     680     324     268
 
TatI w'GTAC_w

Cuts at:      0     962    1595    2598 
   Size:        962     633    1003

  Fragments arranged by size: 

                1003     962     633
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     772     804    1406    1438    1922    1954    2598 
   Size:        772      32     602      32     484      32     644

  Fragments arranged by size: 

                 772     644     602     484      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1180    2598 
   Size:       1180    1418
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     621     993    1908    2598 
   Size:        621     372     915     690

  Fragments arranged by size: 

                 915     690     621     372
 
XhoI C'TCGA_G

Cuts at:      0     325    2598 
   Size:        325    2273
 
XmaI C'CCGG_G

Cuts at:      0      33      81    1683    1967    2124    2598 
   Size:         33      48    1602     284     157     474

  Fragments arranged by size: 

                1602     474     284     157      48      33
 
XmnI GAAnn'nnTTC

Cuts at:      0     935    2598 
   Size:        935    1663
 
ZraI GAC'GTC

Cuts at:      0    1536    2598 
   Size:       1536    1062
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AleI    AlwNI
     ApaI     ApoI     AvaI     BanI    BanII     BbeI    BfrBI     BglI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI
   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    HaeII    Hin4I   HincII     KasI     KpnI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NsiI
     NspI     PasI    PflMI     PfoI     PpiI    PpuMI   PspOMI    PspXI
     PstI    PvuII     SacI    SacII    SanDI     SapI     SfcI     SfiI
     SfoI     SmaI     SmlI    SnaBI     SphI     SrfI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AfeI    AflII   AflIII     AgeI     AhdI     AlfI     AloI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI
     BcgI    BciVI     BclI    BglII     BlpI    BpuEI    BsaBI    BsiEI
    BsiWI     BsmI    BsrDI    BsrGI    BssSI  BstZ17I    BtgZI     ClaI
    CspCI     DraI     EagI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     HpaI     MfeI     MluI     NdeI     NotI     NruI
     PacI     PciI     PmeI     PmlI    PshAI     PsiI     PsrI     PvuI
    RsrII     SalI     SbfI     ScaI    SexAI     SgfI    SgrAI     SpeI
     SspI     SwaI     XbaI
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