Restriction map for pF1KB6756
##################################
# NO CUT ENZYMES (  159 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbvCI       BciVI       BcnI        BfaI        
BfrI        BglII       BlnI        BpmI        BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Cfr10I      ClaI        CpoI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRI       EcoRV       FseI        FspI        HapII       
HgaI        Hin1I       HindIII     HpaI        HpaII       
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeIII      MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MspI        
MunI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tsp45I      
Tsp509I     Tth111I     Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccII         421  CGCG                 CG^CG
AfaI          228  GTAC                 GT^AC
AflIII        257  ACGTGT               A^C[GA][CT]GT
AlwNI         101  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          126  CCTCAGG              CC^T[ACGT]AGG
AspHI         133  GAGCTC               G[AT]GC[AT]^C
AvaII         390  GGACC                G^G[AT]CC
BanII         133  GAGCTC               G[GA]GC[CT]^C
BbsI          282  GAAGAC               GAAGAC(2/6)
BcgI          269  GCAGTGCCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          251  TGATCA               T^GATCA
BglI           80  GCCTTGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          297  GCTGAGC              GC^T[ACGT]AGC
BmyI          133  GAGCTC               G[AGT]GC[ACT]^C
Bpu1102I      297  GCTGAGC              GC^T[ACGT]AGC
BpuAI         282  GAAGAC               GAAGAC(2/6)
BsaI          321  GGTCTC               GGTCTC(1/5)
BsgI          233  GTGCAG               GTGCAG(16/14)
BsiHKAI       133  GAGCTC               G[AT]GC[AT]^C
BsiYI         312  CCCGCACAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          312  CCCGCACAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         426  GGGAC                GGGAC(10/14)
Bsp1286I      133  GAGCTC               G[AGT]GC[ACT]^C
BspMI         392  ACCTGC               ACCTGC(4/8)
BstUI         421  CGCG                 CG^CG
Bsu36I        126  CCTCAGG              CC^T[ACGT]AGG
CelII         297  GCTGAGC              GC^T[ACGT]AGC
CfoI           46  GCGC                 GCG^C
DpnII         252  GATC                 ^GATC
DraI          405  TTTAAA               TTT^AAA
DsaI           59  CCACGG               C^C[GA][CT]GG
Eco81I        126  CCTCAGG              CC^T[ACGT]AGG
EcoT14I        81  CCTTGG               C^C[AT][AT]GG
EcoT22I         1  ATGCAT               ATGCA^T
FauI          312  CCCGC                CCCGC(4/6)
FbaI          251  TGATCA               T^GATCA
HaeII          45  GGCGCT               [GA]GCGC^[CT]
HhaI           46  GCGC                 GCG^C
HinP1I         46  GCGC                 G^CGC
HphI          158  TCACC                GGTGA(8/7)
MaeII         257  ACGT                 A^CGT
MboI          252  GATC                 ^GATC
MboII         282  GAAGA                GAAGA(8/7)
MseI          406  TTAA                 T^TAA
MvnI          421  CGCG                 CG^CG
NdeII         252  GATC                 ^GATC
NlaIV         174  GGCTCC               GG[ACGT]^[ACGT]CC
NruI          420  TCGCGA               TCG^CGA
NsiI            1  ATGCAT               ATGCA^T
PpuMI         389  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI          57  GACCACGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          267  CTGCAG               CTGCA^G
RsaI          228  GTAC                 GT^AC
SacI          133  GAGCTC               GAGCT^C
Sau3AI        252  GATC                 ^GATC
StyI           81  CCTTGG               C^C[AT][AT]GG
Tru9I         406  TTAA                 T^TAA
XcmI          362  CCAAAATCGAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

BseRI          10  CTCCTC               GAGGAG(10/8)
              142  GAGGAG              
BsmAI         356  GAGAC                GTCTC(1/5)
              322  GTCTC               
BsrDI          66  CATTGC               GCAATG(2/0)
              185  GCAATG              
BsrI           33  ACTGG                ACTGG(1/-1)
              378  CCAGT               
BssKI         100  CCAGG                ^CC[ACGT]GG
              240  CCTGG               
BstF5I          4  CATCC                GGATG(2/0)
              293  GGATG               
BstNI         100  CCAGG                CC^[AT]GG
              240  CCTGG               
DraII         102  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              389  AGGACCT             
EcoO109I      102  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              389  AGGACCT             
EcoRII        100  CCAGG                ^CC[AT]GG
              240  CCTGG               
FokI            4  CATCC                GGATG(9/13)
              293  GGATG               
HincII         54  GTTGAC               GT[CT]^[GA]AC
              151  GTCAAC              
HindII         54  GTTGAC               GT[CT]^[GA]AC
              151  GTCAAC              
HinfI         244  GAATC                G^A[ACGT]TC
              305  GATTC               
MspA1I        218  CAGCTG               C[AC]G^C[GT]G
              326  CAGCTG              
MvaI          100  CCAGG                CC^[AT]GG
              240  CCTGG               
NspI          224  GCATGT               [GA]CATG^[CT]
              348  GCATGT              
PvuII         218  CAGCTG               CAG^CTG
              326  CAGCTG              
ScrFI         100  CCAGG                CC^[ACGT]GG
              240  CCTGG               
SfcI          119  CTACAG               C^T[GA][CT]AG
              267  CTGCAG              
TaqI          278  TCGA                 T^CGA
              368  TCGA                
TfiI          244  GAATC                G^A[AT]TC
              305  GATTC               
TspRI          32  CACTG                CA[GC]TG[ACGT][ACGT]^
              270  CAGTG               
TthHB8I       278  TCGA                 T^CGA
              368  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AciI        AluI        BbvI        BsaJI       BsrBI       
Cac8I       Cfr13I      DdeI        Fnu4HI      HaeIII      
ItaI        MnlI        MwoI        NlaIII      Sau96I      
SfaNI       TseI        
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