(Linear) (Six-base) MAPSORT of: KIEE0224.seq  Check: 1147  from: 1  to: 267



KIEE0224.orf

 With 182 enzymes: SgfI * 

                        July 23, 2008 09:45  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     267 
   Size:         31     236
 
AflIII A'CryG_T

Cuts at:      0     115     267 
   Size:        115     152
 
AlwNI CAG_nnn'CTG

Cuts at:      0     132     267 
   Size:        132     135
 
ApoI r'AATT_y

Cuts at:      0     138     267 
   Size:        138     129
 
BamHI G'GATC_C

Cuts at:      0     196     267 
   Size:        196      71
 
BanI G'GyrC_C

Cuts at:      0     169     267 
   Size:        169      98
 
BsiHKAI G_wGCw'C

Cuts at:      0      38     267 
   Size:         38     229
 
BsmI GAATG_Cn'

Cuts at:      0     225     267 
   Size:        225      42
 
Bsp1286I G_dGCh'C

Cuts at:      0      38     267 
   Size:         38     229
 
BstYI r'GATC_y

Cuts at:      0     196     267 
   Size:        196      71
 
BtsI GCAGTG_nn'

Cuts at:      0     107     267 
   Size:        107     160
 
DraI TTT'AAA

Cuts at:      0     236     267 
   Size:        236      31
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     267 
   Size:         31     236
 
HaeII r_GCGC'y

Cuts at:      0     132     267 
   Size:        132     135
 
NdeI CA'TA_TG

Cuts at:      0     207     267 
   Size:        207      60
 
NspI r_CATG'y

Cuts at:      0     119     267 
   Size:        119     148
 
PciI A'CATG_T

Cuts at:      0     115     267 
   Size:        115     152
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      84     267 
   Size:         84     183
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI    BamHI     BanI  BsiHKAI     BsmI
 Bsp1286I    BstYI     BtsI     DraI  Eco57MI    HaeII     NdeI     NspI
     PciI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI