Restriction map for pF1KB6308
 (Linear) (Six-base) MAPSORT of: KIBB6308.seq  Check: 4096  from: 1  to: 840



KIBB6308.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     704     799     840 
   Size:        704      95      41

  Fragments arranged by size: 

                 704      95      41
 
AflIII A'CryG_T

Cuts at:      0     806     840 
   Size:        806      34
 
AgeI A'CCGG_T

Cuts at:      0     444     840 
   Size:        444     396
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     434     466     840 
   Size:        434      32     374

  Fragments arranged by size: 

                 434     374      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     460     840 
   Size:        460     380
 
ApaLI G'TGCA_C

Cuts at:      0     688     840 
   Size:        688     152
 
ApoI r'AATT_y

Cuts at:      0     196     570     840 
   Size:        196     374     270

  Fragments arranged by size: 

                 374     270     196
 
AvaI C'yCGr_G

Cuts at:      0     266     840 
   Size:        266     574
 
AvrII C'CTAG_G

Cuts at:      0     415     840 
   Size:        415     425
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     283     496     809     840 
   Size:        283     213     313      31

  Fragments arranged by size: 

                 313     283     213      31
 
BglII A'GATC_T

Cuts at:      0     618     840 
   Size:        618     222
 
Bme1580I G_kGCm'C

Cuts at:      0     692     840 
   Size:        692     148
 
BmgBI CAC'GTC

Cuts at:      0     807     840 
   Size:        807      33
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     231     840 
   Size:        231     609
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     348     840 
   Size:        348     492
 
BsaWI w'CCGG_w

Cuts at:      0     444     840 
   Size:        444     396
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     164     840 
   Size:        164     676
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      72     840 
   Size:         72     768
 
BsiHKAI G_wGCw'C

Cuts at:      0     692     840 
   Size:        692     148
 
BsmI GAATG_Cn'

Cuts at:      0     294     840 
   Size:        294     546
 
Bsp1286I G_dGCh'C

Cuts at:      0     692     840 
   Size:        692     148
 
BspHI T'CATG_A

Cuts at:      0     798     840 
   Size:        798      42
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      94     840 
   Size:         94     746
 
BsrFI r'CCGG_y

Cuts at:      0     444     840 
   Size:        444     396
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      21     840 
   Size:         21     819
 
BstYI r'GATC_y

Cuts at:      0     456     618     840 
   Size:        456     162     222

  Fragments arranged by size: 

                 456     222     162
 
BtgI C'CryG_G

Cuts at:      0     347     840 
   Size:        347     493
 
DraI TTT'AAA

Cuts at:      0     615     840 
   Size:        615     225
 
EarI CTCTTCn'nnn_

Cuts at:      0     493     840 
   Size:        493     347
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     704     799     840 
   Size:        704      95      41

  Fragments arranged by size: 

                 704      95      41
 
EcoO109I rG'GnC_Cy

Cuts at:      0      29     840 
   Size:         29     811
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     135     840 
   Size:        135     705
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     235     267     343     375     840 
   Size:        235      32      76      32     465

  Fragments arranged by size: 

                 465     235      76      32      32
 
HindIII A'AGCT_T

Cuts at:      0      73     112     840 
   Size:         73      39     728

  Fragments arranged by size: 

                 728      73      39
 
MslI CAynn'nnrTG

Cuts at:      0     406     840 
   Size:        406     434
 
NspI r_CATG'y

Cuts at:      0     162     840 
   Size:        162     678
 
PstI C_TGCA'G

Cuts at:      0     222     840 
   Size:        222     618
 
SfcI C'TryA_G

Cuts at:      0     218     712     840 
   Size:        218     494     128

  Fragments arranged by size: 

                 494     218     128
 
SmlI C'TyrA_G

Cuts at:      0     266     840 
   Size:        266     574
 
SspI AAT'ATT

Cuts at:      0     762     840 
   Size:        762      78
 
StuI AGG'CCT

Cuts at:      0     751     840 
   Size:        751      89
 
StyI C'CwwG_G

Cuts at:      0     415     840 
   Size:        415     425
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     667     840 
   Size:        667     173
 
XhoI C'TCGA_G

Cuts at:      0     266     840 
   Size:        266     574
 
XmnI GAAnn'nnTTC

Cuts at:      0      43     187     840 
   Size:         43     144     653

  Fragments arranged by size: 

                 653     144      43
 

 Enzymes that do cut:

     AcuI   AflIII     AgeI     AloI    AlwNI    ApaLI     ApoI     AvaI
    AvrII     BbsI    BglII Bme1580I    BmgBI     BplI     BsaI    BsaWI
    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrFI
    BstXI    BstYI     BtgI     DraI     EarI  Eco57MI EcoO109I     FalI
    Hin4I  HindIII     MslI     NspI     PstI     SfcI     SmlI     SspI
     StuI     StyI    TaqII     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AhdI
     AleI     AlfI     ApaI     AscI     AseI    AsiSI     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI     BmrI     BmtI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaXI    BseYI    BsiEI    BsiWI    BsmBI    BspEI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com