Restriction map for pF1KE0342
##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

Acc65I      AccI        AclI        AcsI        AflII       
AhdI        ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvrII       BamHI       
BbeI        BbrPI       BcgI        BciVI       BclI        
BfrI        BlnI        BsaAI       BsaI        BsgI        
BsiWI       Bsp1407I    BspDI       BspHI       BspLU11I    
BsrGI       BssHII      Bst1107I    BstBI       BstZ17I     
ClaI        CpoI        DrdI        Eam1105I    EarI        
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MluI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TfiI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII         262  GACGTC               GACGT^C
AccII         379  CGCG                 CG^CG
AgeI         1169  ACCGGT               A^CCGGT
Alw44I        835  GTGCAC               G^TGCAC
Aor51HI       352  AGCGCT               AGC^GCT
ApaLI         835  GTGCAC               G^TGCAC
Asp700I       867  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII         953  TGCGCA               TGC^GCA
BbsI         1035  GAAGAC               GAAGAC(2/6)
BbvCI         163  CCTCAGC              CCTCAGC(-5/-2)
BglI         1133  GCCAGGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         212  AGATCT               A^GATCT
BpuAI        1035  GAAGAC               GAAGAC(2/6)
BsaBI        1743  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        1690  CGGCCG               CG[GA][CT]^CG
BsrBI         553  GAGCGG               CCGCTC(-3/-3)
BsrDI         984  CATTGC               GCAATG(2/0)
BstEII        513  GGTGACC              G^GT[ACGT]ACC
BstPI         513  GGTGACC              G^GT[ACGT]ACC
BstUI         379  CGCG                 CG^CG
DraI          217  TTTAAA               TTT^AAA
EagI         1690  CGGCCG               C^GGCCG
EclXI        1690  CGGCCG               C^GGCCG
Eco47III      352  AGCGCT               AGC^GCT
Eco52I       1690  CGGCCG               C^GGCCG
EcoNI         114  CCTGGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       513  GGTGACC              G^GT[ACGT]ACC
FspI          953  TGCGCA               TGC^GCA
HinfI        1125  GACTC                G^A[ACGT]TC
MamI         1743  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1285  CAATTG               C^AATTG
MseI          218  TTAA                 T^TAA
MunI         1285  CAATTG               C^AATTG
MvnI          379  CGCG                 CG^CG
NspI         1155  GCATGT               [GA]CATG^[CT]
PaeR7I        640  CTCGAG               C^TCGAG
PflMI         972  CCAGGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1169  ACCGGT               A^CCGGT
PleI         1125  GACTC                GAGTC(4/5)
SexAI        1472  ACCTGGT              A^CC[AT]GGT
Tru9I         218  TTAA                 T^TAA
Tsp509I      1286  AATT                 ^AATT
Van91I        972  CCAGGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          640  CTCGAG               C^TCGAG
XmnI          867  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccIII       1205  TCCGGA               T^CCGGA
             1571  TCCGGA              
AflIII        805  ACGTGT               A^C[GA][CT]GT
             1313  ACGTGT              
AlwI         1560  GATCC                GGATC(4/5)
             1208  GGATC               
AocI         1015  CCTGAGG              CC^T[ACGT]AGG
             1587  CCTCAGG             
BlpI          541  GCTGAGC              GC^T[ACGT]AGC
             1008  GCTGAGC             
Bpu1102I      541  GCTGAGC              GC^T[ACGT]AGC
             1008  GCTGAGC             
BseAI        1205  TCCGGA               T^CCGGA
             1571  TCCGGA              
BsmI         1548  GCATTC               GAATGC(1/-1)
             1679  GCATTC              
BspEI        1205  TCCGGA               T^CCGGA
             1571  TCCGGA              
BstAPI        231  GCAGAACGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              576  GCAGGTGCTGC         
BstXI         854  CCAAGCAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1334  CCATTGGCCTGG        
BstYI         212  AGATCT               [GA]^GATC[CT]
             1208  GGATCT              
Bsu36I       1015  CCTGAGG              CC^T[ACGT]AGG
             1587  CCTCAGG             
CelII         541  GCTGAGC              GC^T[ACGT]AGC
             1008  GCTGAGC             
Eco81I       1015  CCTGAGG              CC^T[ACGT]AGG
             1587  CCTCAGG             
HgaI         1405  GACGC                GACGC(5/10)
              743  GCGTC               
MboII        1035  GAAGA                GAAGA(8/7)
             1226  TCTTC               
MflI          212  AGATCT               [GA]^GATC[CT]
             1208  GGATCT              
MroI         1205  TCCGGA               T^CCGGA
             1571  TCCGGA              
MslI         1482  CACCCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1771  CACCCCAATG          
PvuII         562  CAGCTG               CAG^CTG
              943  CAGCTG              
SmlI          550  CTTGAG               C^T[CT][GA]AG
              640  CTCGAG              
StuI          665  AGGCCT               AGG^CCT
              869  AGGCCT              
XcmI          851  CCACCAAGCAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1553  CCAAGGTGATCCTGG     
XhoII         212  AGATCT               [GA]^GATC[CT]
             1208  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwNI       
ApaI        AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbvI        BcnI        BfaI        
BmyI        BpmI        BsaHI       BsaJI       BsaWI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrFI       BsrI        BssKI       BssSI       BstF5I      
BstNI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DraIII      DsaI        
EaeI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        Hin1I       HinP1I      HpaII       HphI        
ItaI        MaeI        MaeII       MaeIII      MboI        
MluNI       MnlI        MscI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       PpuMI       PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        StyI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     XmaCI       XmaI        
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