Restriction map for pF1KE0342
 (Linear) (Six-base) MAPSORT of: KIEE0342.seq  Check: 412  from: 1  to: 1875



KIEE0342.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     567     849    1875 
   Size:        567     282    1026

  Fragments arranged by size: 

                1026     567     282
 
AatII G_ACGT'C

Cuts at:      0     266    1875 
   Size:        266    1609
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     240    1054    1875 
   Size:        240     814     821

  Fragments arranged by size: 

                 821     814     240
 
AfeI AGC'GCT

Cuts at:      0     354    1875 
   Size:        354    1521
 
AflIII A'CryG_T

Cuts at:      0     805    1313    1875 
   Size:        805     508     562

  Fragments arranged by size: 

                 805     562     508
 
AgeI A'CCGG_T

Cuts at:      0    1169    1875 
   Size:       1169     706
 
AleI CACnn'nnGTG

Cuts at:      0    1486    1875 
   Size:       1486     389
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     608    1875 
   Size:        608    1267
 
AlwNI CAG_nnn'CTG

Cuts at:      0     582    1008    1396    1875 
   Size:        582     426     388     479

  Fragments arranged by size: 

                 582     479     426     388
 
ApaI G_GGCC'C

Cuts at:      0      28    1284    1799    1875 
   Size:         28    1256     515      76

  Fragments arranged by size: 

                1256     515      76      28
 
ApaLI G'TGCA_C

Cuts at:      0     835    1875 
   Size:        835    1040
 
AvaI C'yCGr_G

Cuts at:      0     169     640    1409    1619    1875 
   Size:        169     471     769     210     256

  Fragments arranged by size: 

                 769     471     256     210     169
 
BanI G'GyrC_C

Cuts at:      0      90     391    1324    1330    1476    1875 
   Size:         90     301     933       6     146     399

  Fragments arranged by size: 

                 933     399     301     146      90       6
 
BanII G_rGCy'C

Cuts at:      0      28     176    1015    1284    1659    1799    1875 
   Size:         28     148     839     269     375     140      76

  Fragments arranged by size: 

                 839     375     269     148     140      76      28
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1042    1875 
   Size:       1042     833
 
BbvCI CC'TCA_GC

Cuts at:      0     164    1875 
   Size:        164    1711
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1139    1875 
   Size:       1139     736
 
BglII A'GATC_T

Cuts at:      0     212    1875 
   Size:        212    1663
 
BlpI GC'TnA_GC

Cuts at:      0     542    1009    1875 
   Size:        542     467     866

  Fragments arranged by size: 

                 866     542     467
 
Bme1580I G_kGCm'C

Cuts at:      0      28      69      95     396     536     839    1284    1481 
   Size:         28      41      26     301     140     303     445     197

Cuts at:   1481    1799    1875 
   Size:        318      76

  Fragments arranged by size: 

                 445     318     303     301     197     140      76      41
                  28      26
 
BmgBI CAC'GTC

Cuts at:      0     510     806    1314    1875 
   Size:        510     296     508     561

  Fragments arranged by size: 

                 561     510     508     296
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1287    1865    1875 
   Size:       1287     578      10

  Fragments arranged by size: 

                1287     578      10
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     244     586     700    1171    1793    1875 
   Size:        136     108     342     114     471     622      82

  Fragments arranged by size: 

                 622     471     342     136     114     108      82
 
Bpu10I CC'TnA_GC

Cuts at:      0      70     164    1875 
   Size:         70      94    1711

  Fragments arranged by size: 

                1711      94      70
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     571    1875 
   Size:        571    1304
 
BsaBI GATnn'nnATC

Cuts at:      0    1747    1875 
   Size:       1747     128
 
BsaHI Gr'CG_yC

Cuts at:      0     263     743    1406    1875 
   Size:        263     480     663     469

  Fragments arranged by size: 

                 663     480     469     263
 
BsaWI w'CCGG_w

Cuts at:      0     887    1169    1205    1571    1875 
   Size:        887     282      36     366     304

  Fragments arranged by size: 

                 887     366     304     282      36
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     133     474    1033    1123    1875 
   Size:        133     341     559      90     752

  Fragments arranged by size: 

                 752     559     341     133      90
 
BseYI C'CCAG_C

Cuts at:      0     139     701    1162    1505    1875 
   Size:        139     562     461     343     370

  Fragments arranged by size: 

                 562     461     370     343     139
 
BsiEI CG_ry'CG

Cuts at:      0    1693    1875 
   Size:       1693     182
 
BsiHKAI G_wGCw'C

Cuts at:      0     613     839    1259    1772    1875 
   Size:        613     226     420     513     103

  Fragments arranged by size: 

                 613     513     420     226     103
 
BsmI GAATG_Cn'

Cuts at:      0    1548    1679    1875 
   Size:       1548     131     196

  Fragments arranged by size: 

                1548     196     131
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      90     913    1611    1875 
   Size:         90     823     698     264

  Fragments arranged by size: 

                 823     698     264      90
 
Bsp1286I G_dGCh'C

Cuts at:      0      28      69      95     176     396     536     613     839 
   Size:         28      41      26      81     220     140      77     226

Cuts at:    839    1015    1259    1284    1481    1659    1772    1799    1875 
   Size:        176     244      25     197     178     113      27      76

  Fragments arranged by size: 

                 244     226     220     197     178     176     140     113
                  81      77      76      41      28      27      26      25
            
 
BspEI T'CCGG_A

Cuts at:      0    1205    1571    1875 
   Size:       1205     366     304

  Fragments arranged by size: 

                1205     366     304
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     567     849    1099    1196    1875 
   Size:        567     282     250      97     679

  Fragments arranged by size: 

                 679     567     282     250      97
 
BsrBI CCG'CTC

Cuts at:      0     555    1875 
   Size:        555    1320
 
BsrDI GCAATG_nn'

Cuts at:      0     983    1875 
   Size:        983     892
 
BsrFI r'CCGG_y

Cuts at:      0    1169    1327    1692    1875 
   Size:       1169     158     365     183

  Fragments arranged by size: 

                1169     365     183     158
 
BssSI C'ACGA_G

Cuts at:      0     497    1180    1513    1875 
   Size:        497     683     333     362

  Fragments arranged by size: 

                 683     497     362     333
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     237     582    1875 
   Size:        237     345    1293

  Fragments arranged by size: 

                1293     345     237
 
BstEII G'GTnAC_C

Cuts at:      0     513    1875 
   Size:        513    1362
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     861    1341    1875 
   Size:        861     480     534

  Fragments arranged by size: 

                 861     534     480
 
BstYI r'GATC_y

Cuts at:      0     212    1208    1875 
   Size:        212     996     667

  Fragments arranged by size: 

                 996     667     212
 
Bsu36I CC'TnA_GG

Cuts at:      0    1016    1588    1875 
   Size:       1016     572     287

  Fragments arranged by size: 

                1016     572     287
 
BtgI C'CryG_G

Cuts at:      0      35     449     770     977    1628    1875 
   Size:         35     414     321     207     651     247

  Fragments arranged by size: 

                 651     414     321     247     207      35
 
BtsI GCAGTG_nn'

Cuts at:      0      69    1452    1715    1875 
   Size:         69    1383     263     160

  Fragments arranged by size: 

                1383     263     160      69
 
DraI TTT'AAA

Cuts at:      0     219    1875 
   Size:        219    1656
 
DraIII CAC_nnn'GTG

Cuts at:      0     418    1515    1537    1875 
   Size:        418    1097      22     338

  Fragments arranged by size: 

                1097     418     338      22
 
EaeI y'GGCC_r

Cuts at:      0      48     452     503     980    1517    1565    1690    1875 
   Size:         48     404      51     477     537      48     125     185

  Fragments arranged by size: 

                 537     477     404     185     125      51      48      48
            
 
EagI C'GGCC_G

Cuts at:      0    1690    1875 
   Size:       1690     185
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     240     244     586     700    1054    1171    1793 
   Size:        136     104       4     342     114     354     117     622

Cuts at:   1793    1875 
   Size:         82

  Fragments arranged by size: 

                 622     354     342     136     117     114     104      82
                   4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     118    1875 
   Size:        118    1757
 
EcoO109I rG'GnC_Cy

Cuts at:      0      24     160     372    1140    1185    1470    1795    1796 
   Size:         24     136     212     768      45     285     325       1

Cuts at:   1796    1828    1859    1875 
   Size:         32      31      16

  Fragments arranged by size: 

                 768     325     285     212     136      45      32      31
                  24      16       1
 
FspI TGC'GCA

Cuts at:      0     955    1875 
   Size:        955     920
 
FspAI rTGC'GCAy

Cuts at:      0     955    1875 
   Size:        955     920
 
HaeII r_GCGC'y

Cuts at:      0     140     273     356    1875 
   Size:        140     133      83    1519

  Fragments arranged by size: 

                1519     140     133      83
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     699     731    1875 
   Size:        699      32    1144

  Fragments arranged by size: 

                1144     699      32
 
MfeI C'AATT_G

Cuts at:      0    1285    1875 
   Size:       1285     590
 
MscI TGG'CCA

Cuts at:      0      50     454     982    1519    1567    1875 
   Size:         50     404     528     537      48     308

  Fragments arranged by size: 

                 537     528     404     308      50      48
 
MslI CAynn'nnrTG

Cuts at:      0    1486    1775    1875 
   Size:       1486     289     100

  Fragments arranged by size: 

                1486     289     100
 
MspA1I CmG'CkG

Cuts at:      0     564     678     945    1875 
   Size:        564     114     267     930

  Fragments arranged by size: 

                 930     564     267     114
 
NcoI C'CATG_G

Cuts at:      0      35     449     977    1628    1875 
   Size:         35     414     528     651     247

  Fragments arranged by size: 

                 651     528     414     247      35
 
NspI r_CATG'y

Cuts at:      0    1159    1875 
   Size:       1159     716
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     978    1875 
   Size:        978     897
 
PfoI T'CCnGG_A

Cuts at:      0     875    1271    1523    1875 
   Size:        875     396     252     352

  Fragments arranged by size: 

                 875     396     352     252
 
PpuMI rG'GwC_Cy

Cuts at:      0    1185    1470    1828    1859    1875 
   Size:       1185     285     358      31      16

  Fragments arranged by size: 

                1185     358     285      31      16
 
PspOMI G'GGCC_C

Cuts at:      0      24    1280    1795    1875 
   Size:         24    1256     515      80

  Fragments arranged by size: 

                1256     515      80      24
 
PspXI vC'TCGA_Gb

Cuts at:      0     640    1875 
   Size:        640    1235
 
PstI C_TGCA'G

Cuts at:      0     102     233     296     360     587    1654    1875 
   Size:        102     131      63      64     227    1067     221

  Fragments arranged by size: 

                1067     227     221     131     102      64      63
 
PvuII CAG'CTG

Cuts at:      0     564     945    1875 
   Size:        564     381     930

  Fragments arranged by size: 

                 930     564     381
 
SexAI A'CCwGG_T

Cuts at:      0    1472    1875 
   Size:       1472     403
 
SfcI C'TryA_G

Cuts at:      0      98     149     177     229     292     356     583    1650 
   Size:         98      51      28      52      63      64     227    1067

Cuts at:   1650    1875 
   Size:        225

  Fragments arranged by size: 

                1067     227     225      98      64      63      52      51
                  28
 
SmaI CCC'GGG

Cuts at:      0     171    1411    1621    1875 
   Size:        171    1240     210     254

  Fragments arranged by size: 

                1240     254     210     171
 
SmlI C'TyrA_G

Cuts at:      0     550     640    1875 
   Size:        550      90    1235

  Fragments arranged by size: 

                1235     550      90
 
SrfI GCCC'GGGC

Cuts at:      0     171    1875 
   Size:        171    1704
 
StuI AGG'CCT

Cuts at:      0     667     871    1875 
   Size:        667     204    1004

  Fragments arranged by size: 

                1004     667     204
 
StyI C'CwwG_G

Cuts at:      0      35     437     449     596     977    1553    1628    1875 
   Size:         35     402      12     147     381     576      75     247

  Fragments arranged by size: 

                 576     402     381     247     147      75      35      12
            
 
TatI w'GTAC_w

Cuts at:      0     791    1875 
   Size:        791    1084
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     858    1560    1875 
   Size:        858     702     315

  Fragments arranged by size: 

                 858     702     315
 
XhoI C'TCGA_G

Cuts at:      0     640    1875 
   Size:        640    1235
 
XmaI C'CCGG_G

Cuts at:      0     169    1409    1619    1875 
   Size:        169    1240     210     256

  Fragments arranged by size: 

                1240     256     210     169
 
XmnI GAAnn'nnTTC

Cuts at:      0     871    1875 
   Size:        871    1004
 
ZraI GAC'GTC

Cuts at:      0     264    1875 
   Size:        264    1611
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI   AflIII     AgeI     AleI     AlfI
    AlwNI     ApaI    ApaLI     AvaI     BanI    BanII     BbsI    BbvCI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
    BpuEI    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI
     DraI   DraIII     EaeI     EagI  Eco57MI    EcoNI EcoO109I     FspI
    FspAI    HaeII    Hin4I     MfeI     MscI     MslI   MspA1I     NcoI
     NspI    PflMI     PfoI    PpuMI   PspOMI    PspXI     PstI    PvuII
    SexAI     SfcI     SmaI     SmlI     SrfI     StuI     StyI     TatI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII     AhdI     AloI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BcgI    BciVI
     BclI    BfrBI     BmtI     BplI     BsaI    BsaAI    BsaXI     BsgI
    BsiWI    BspHI    BsrGI   BssHII    BstBI  BstZ17I    BtgZI     ClaI
    CspCI     DrdI     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI
     FseI   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SspI     SwaI    TaqII     TstI  Tth111I     XbaI
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