Restriction map for
pF1KB7821
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BcnI BfrI BlnI
BlpI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmAI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI BstEII
BstPI BstUI Bsu36I CelII CfoI
ClaI CpoI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MfeI MluI MroI MslI
MspA1I MunI MvnI NarI NciI
NcoI NdeI NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
Sse8387I SspBI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 1332 GTATAC GT^[AC][GT]AC
AciI 1337 CCGC CCGC(-3/-1)
Asp700I 139 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 395 GTGCTC G[AT]GC[AT]^C
AvaII 259 GGTCC G^G[AT]CC
BalI 96 TGGCCA TGG^CCA
BanII 617 GAGCCC G[GA]GC[CT]^C
BbsI 1139 GAAGAC GAAGAC(2/6)
BbvCI 30 CCTCAGC CCTCAGC(-5/-2)
BglI 89 GCCAGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1161 AGATCT A^GATCT
BpuAI 1139 GAAGAC GAAGAC(2/6)
BsaBI 664 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 712 CCAAGG C^C[ACGT][ACGT]GG
BsiHKAI 395 GTGCTC G[AT]GC[AT]^C
BsiYI 468 CCTTTGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 468 CCTTTGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 465 GTCCC GGGAC(10/14)
BsmI 1354 GCATTC GAATGC(1/-1)
BsrFI 21 GCCGGC [GA]^CCGG[CT]
Bst1107I 1332 GTATAC GTA^TAC
BstAPI 496 GCAAAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 981 CCAGCTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1332 GTATAC GTA^TAC
Cfr10I 21 GCCGGC [GA]^CCGG[CT]
Cfr13I 259 GGTCC G^G[ACGT]CC
EaeI 96 TGGCCA [CT]^GGCC[GA]
EarI 1285 GAAGAG CTCTTC(1/4)
EcoNI 468 CCTTTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 712 CCAAGG C^C[AT][AT]GG
HapII 22 CCGG C^CGG
HgaI 45 GACGC GACGC(5/10)
HpaII 22 CCGG C^CGG
HphI 80 TCACC GGTGA(8/7)
Ksp632I 1285 GAAGAG CTCTTC(1/4)
MamI 664 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 96 TGGCCA TGG^CCA
MscI 96 TGGCCA TGG^CCA
MspI 22 CCGG C^CGG
NaeI 21 GCCGGC GCC^GGC
NgoMIV 21 GCCGGC G^CCGGC
NspI 501 GCATGC [GA]CATG^[CT]
PstI 1104 CTGCAG CTGCA^G
Sau96I 259 GGTCC G^G[ACGT]CC
SpeI 1183 ACTAGT A^CTAGT
SphI 501 GCATGC GCATG^C
SspI 152 AATATT AAT^ATT
StuI 592 AGGCCT AGG^CCT
StyI 712 CCAAGG C^C[AT][AT]GG
Tsp45I 1021 GTGAC ^GT[GC]AC
XmnI 139 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwI 820 GATCC GGATC(4/5)
1015 GATCC
BmyI 395 GTGCTC G[AGT]GC[ACT]^C
617 GAGCCC
BpmI 84 CTGGAG CTGGAG(16/14)
1507 CTGGAG
BsgI 1571 CTGCAC GTGCAG(16/14)
1192 GTGCAG
Bsp1286I 395 GTGCTC G[AGT]GC[ACT]^C
617 GAGCCC
BstBI 12 TTCGAA TT^CGAA
524 TTCGAA
BstYI 1014 AGATCC [GA]^GATC[CT]
1161 AGATCT
DraI 579 TTTAAA TTT^AAA
705 TTTAAA
HindIII 177 AAGCTT A^AGCTT
1495 AAGCTT
MaeII 17 ACGT A^CGT
585 ACGT
MflI 1014 AGATCC [GA]^GATC[CT]
1161 AGATCT
NspV 12 TTCGAA TT^CGAA
524 TTCGAA
PleI 229 GACTC GAGTC(4/5)
463 GAGTC
SfaNI 276 GCATC GCATC(5/9)
1405 GCATC
SfuI 12 TTCGAA TT^CGAA
524 TTCGAA
XhoII 1014 AGATCC [GA]^GATC[CT]
1161 AGATCT
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BfaI BsrI BssKI BstF5I BstNI
Cac8I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HinfI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI ScrFI SfcI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I