Restriction map for pF1KB7811
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AgeI        AhdI        AocI        Aor51HI     
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AviII       AvrII       BcgI        BclI        
BfaI        BfrI        BglII       BlnI        BsaBI       
BsaI        BsiWI       BsmI        Bsp1407I    BspDI       
BspHI       BsrDI       BsrGI       BssHII      Bst1107I    
BstBI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraIII      EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRI       
EcoRV       EcoT22I     FbaI        FspI        HindIII     
HpaI        KpnI        KspI        MaeI        MamI        
MfeI        MluI        MseI        MunI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        Tru9I       
Tsp509I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccIII        233  TCCGGA               T^CCGGA
AfaI          177  GTAC                 GT^AC
Alw44I       1396  GTGCAC               G^TGCAC
ApaLI        1396  GTGCAC               G^TGCAC
Asp700I       123  GAACAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          403  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         602  GGATCC               G^GATCC
BanI          947  GGCGCC               G^G[CT][GA]CC
BbeI          947  GGCGCC               GGCGC^C
BbrPI         133  CACGTG               CAC^GTG
BbsI          382  GAAGAC               GAAGAC(2/6)
BbvCI        1119  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1313  GTATCC               GTATCC(6/5)
BglI         1341  GCCCCCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         382  GAAGAC               GAAGAC(2/6)
BsaAI         133  CACGTG               [CT]AC^GT[GA]
BseAI         233  TCCGGA               T^CCGGA
BsiEI         980  CGACCG               CG[GA][CT]^CG
BsmBI         281  GAGACG               CGTCTC(1/5)
BspEI         233  TCCGGA               T^CCGGA
BspLU11I      221  ACATGT               A^CATGT
BsrBI         117  GAGCGG               CCGCTC(-3/-3)
BssSI          73  CTCGTG               CACGAG(-5/-1)
BstEII       1263  GGTGACC              G^GT[ACGT]ACC
BstPI        1263  GGTGACC              G^GT[ACGT]ACC
EcoO65I      1263  GGTGACC              G^GT[ACGT]ACC
FseI         1075  GGCCGGCC             GGCCGG^CC
HaeII         947  GGCGCC               [GA]GCGC^[CT]
HincII        556  GTCAAC               GT[CT]^[GA]AC
HindII        556  GTCAAC               GT[CT]^[GA]AC
KasI          947  GGCGCC               G^GCGCC
MroI          233  TCCGGA               T^CCGGA
NarI          947  GGCGCC               GG^CGCC
NcoI          827  CCATGG               C^CATGG
PflFI         403  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI          59  CCAAGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         133  CACGTG               CAC^GTG
PmlI          133  CACGTG               CAC^GTG
PstI         1192  CTGCAG               CTGCA^G
RsaI          177  GTAC                 GT^AC
ScaI          176  AGTACT               AGT^ACT
SfoI          947  GGCGCC               GGC^GCC
SmlI          334  CTTGAG               C^T[CT][GA]AG
SphI          534  GCATGC               GCATG^C
StuI         1348  AGGCCT               AGG^CCT
TfiI         1378  GATTC                G^A[AT]TC
Tth111I       403  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I         59  CCAAGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          123  GAACAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccII          24  CGCG                 CG^CG
               96  CGCG                
AcyI          947  GGCGCC               G[GA]^CG[CT]C
             1048  GACGCC              
AflIII        134  ACGTGT               A^C[GA][CT]GT
              221  ACATGT              
AlwNI         571  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1411  CAGCCGCTG           
BlpI          650  GCTCAGC              GC^T[ACGT]AGC
             1203  GCTGAGC             
Bpu1102I      650  GCTCAGC              GC^T[ACGT]AGC
             1203  GCTGAGC             
BsaHI         947  GGCGCC               G[GA]^CG[CT]C
             1048  GACGCC              
BsaWI         233  TCCGGA               [AT]^CCGG[AT]
              599  ACCGGA              
BseRI         895  GAGGAG               GAGGAG(10/8)
              931  GAGGAG              
BsgI          430  GTGCAG               GTGCAG(16/14)
              589  GTGCAG              
BstAPI       1194  GCAGACCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1389  GCAGCTGGTGC         
BstF5I        999  CATCC                GGATG(2/0)
             1164  GGATG               
BstUI          24  CGCG                 CG^CG
               96  CGCG                
BstXI         467  CCAAGCTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              821  CCAGCCCCATGG        
CelII         650  GCTCAGC              GC^T[ACGT]AGC
             1203  GCTGAGC             
DrdI          357  GACCAGTGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1573  GACCCCACTGTC        
EarI          375  GAAGAG               CTCTTC(1/4)
              615  GAAGAG              
FokI          999  CATCC                GGATG(9/13)
             1164  GGATG               
Hin1I         947  GGCGCC               G[GA]^CG[CT]C
             1048  GACGCC              
Ksp632I       375  GAAGAG               CTCTTC(1/4)
              615  GAAGAG              
MslI          487  CATGAGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1159  CACTTGGATG          
MvnI           24  CGCG                 CG^CG
               96  CGCG                
NaeI          677  GCCGGC               GCC^GGC
             1076  GCCGGC              
NgoMIV        677  GCCGGC               G^CCGGC
             1076  GCCGGC              
NspI          221  ACATGT               [GA]CATG^[CT]
              534  GCATGC              
PpuMI        1523  AGGACCC              [GA]G^G[AT]CC[CT]
             1571  AGGACCC             
PvuII         433  CAGCTG               CAG^CTG
             1390  CAGCTG              
SacI         1147  GAGCTC               GAGCT^C
             1321  GAGCTC              
SfcI          738  CTACAG               C^T[GA][CT]AG
             1192  CTGCAG              
SmaI          923  CCCGGG               CCC^GGG
              943  CCCGGG              
TaqI          140  TCGA                 T^CGA
              251  TCGA                
TthHB8I       140  TCGA                 T^CGA
              251  TCGA                
XcmI          869  CCAGCAGCCCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1232  CCAGTGCCCTGCTGG     
XmaCI         923  CCCGGG               C^CCGGG
              943  CCCGGG              
XmaI          923  CCCGGG               C^CCGGG
              943  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AciI        AluI        AlwI        ApaI        AspHI       
AvaI        AvaII       BalI        BanII       BbvI        
BcnI        BmyI        BpmI        BsaJI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstNI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TseI        Tsp45I      TspRI       
XhoII       
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