Restriction map for pF1KB7811
 (Linear) (Six-base) MAPSORT of: KIBB7811.seq  Check: 6497  from: 1  to: 1587



KIBB7811.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394    1203    1587 
   Size:        394     809     384

  Fragments arranged by size: 

                 809     394     384
 
AflIII A'CryG_T

Cuts at:      0     134     221    1587 
   Size:        134      87    1366

  Fragments arranged by size: 

                1366     134      87
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     551    1477    1587 
   Size:        551     926     110

  Fragments arranged by size: 

                 926     551     110
 
AlwNI CAG_nnn'CTG

Cuts at:      0     576    1416    1587 
   Size:        576     840     171

  Fragments arranged by size: 

                 840     576     171
 
ApaI G_GGCC'C

Cuts at:      0      19      35     715    1587 
   Size:         19      16     680     872

  Fragments arranged by size: 

                 872     680      19      16
 
ApaLI G'TGCA_C

Cuts at:      0    1396    1587 
   Size:       1396     191
 
AvaI C'yCGr_G

Cuts at:      0     114     708     923     943    1354    1587 
   Size:        114     594     215      20     411     233

  Fragments arranged by size: 

                 594     411     233     215     114      20
 
BamHI G'GATC_C

Cuts at:      0     602    1587 
   Size:        602     985
 
BanI G'GyrC_C

Cuts at:      0     947    1587 
   Size:        947     640
 
BanII G_rGCy'C

Cuts at:      0      19      35     115     715     902     922    1151    1325 
   Size:         19      16      80     600     187      20     229     174

Cuts at:   1325    1343    1492    1587 
   Size:         18     149      95

  Fragments arranged by size: 

                 600     229     187     174     149      95      80      20
                  19      18      16
 
BbeI G_GCGC'C

Cuts at:      0     951    1587 
   Size:        951     636
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     389    1587 
   Size:        389    1198
 
BbvCI CC'TCA_GC

Cuts at:      0    1120    1587 
   Size:       1120     467
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1324    1587 
   Size:       1324     263
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1347    1587 
   Size:       1347     240
 
BlpI GC'TnA_GC

Cuts at:      0     651    1204    1587 
   Size:        651     553     383

  Fragments arranged by size: 

                 651     553     383
 
Bme1580I G_kGCm'C

Cuts at:      0      19      35     715    1239    1274    1400    1587 
   Size:         19      16     680     524      35     126     187

  Fragments arranged by size: 

                 680     524     187     126      35      19      16
 
BmgBI CAC'GTC

Cuts at:      0     429     699    1587 
   Size:        429     270     888

  Fragments arranged by size: 

                 888     429     270
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     129     712     732    1587 
   Size:        129     583      20     855

  Fragments arranged by size: 

                 855     583     129      20
 
Bpu10I CC'TnA_GC

Cuts at:      0    1120    1587 
   Size:       1120     467
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355    1587 
   Size:        355    1232
 
BsaAI yAC'GTr

Cuts at:      0     135    1587 
   Size:        135    1452
 
BsaHI Gr'CG_yC

Cuts at:      0     948    1049    1587 
   Size:        948     101     538

  Fragments arranged by size: 

                 948     538     101
 
BsaWI w'CCGG_w

Cuts at:      0     233     599    1587 
   Size:        233     366     988

  Fragments arranged by size: 

                 988     366     233
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     730     760    1420    1450    1587 
   Size:        730      30     660      30     137

  Fragments arranged by size: 

                 730     660     137      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     910     946    1587 
   Size:        910      36     641

  Fragments arranged by size: 

                 910     641      36
 
BseYI C'CCAG_C

Cuts at:      0      34     539     732     904    1479    1587 
   Size:         34     505     193     172     575     108

  Fragments arranged by size: 

                 575     505     193     172     108      34
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     451     610    1587 
   Size:        451     159     977

  Fragments arranged by size: 

                 977     451     159
 
BsiEI CG_ry'CG

Cuts at:      0     983    1587 
   Size:        983     604
 
BsiHKAI G_wGCw'C

Cuts at:      0     698    1151    1325    1400    1587 
   Size:        698     453     174      75     187

  Fragments arranged by size: 

                 698     453     187     174      75
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     275    1587 
   Size:        275    1312
 
Bsp1286I G_dGCh'C

Cuts at:      0      19      35     115     698     715     902     922    1151 
   Size:         19      16      80     583      17     187      20     229

Cuts at:   1151    1239    1274    1325    1343    1400    1492    1587 
   Size:         88      35      51      18      57      92      95

  Fragments arranged by size: 

                 583     229     187      95      92      88      80      57
                  51      35      20      19      18      17      16
 
BspEI T'CCGG_A

Cuts at:      0     233    1587 
   Size:        233    1354
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1026    1199    1469    1587 
   Size:       1026     173     270     118

  Fragments arranged by size: 

                1026     270     173     118
 
BsrBI CCG'CTC

Cuts at:      0     119    1587 
   Size:        119    1468
 
BsrFI r'CCGG_y

Cuts at:      0     677    1076    1464    1587 
   Size:        677     399     388     123

  Fragments arranged by size: 

                 677     399     388     123
 
BssSI C'ACGA_G

Cuts at:      0      73    1587 
   Size:         73    1514
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1200    1395    1587 
   Size:       1200     195     192

  Fragments arranged by size: 

                1200     195     192
 
BstEII G'GTnAC_C

Cuts at:      0    1263    1587 
   Size:       1263     324
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     474     828    1587 
   Size:        474     354     759

  Fragments arranged by size: 

                 759     474     354
 
BstYI r'GATC_y

Cuts at:      0       3     392     602     623    1587 
   Size:          3     389     210      21     964

  Fragments arranged by size: 

                 964     389     210      21       3
 
BtgI C'CryG_G

Cuts at:      0      11     702     827     959    1212    1439    1587 
   Size:         11     691     125     132     253     227     148

  Fragments arranged by size: 

                 691     253     227     148     132     125      11
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     363    1579    1587 
   Size:        363    1216       8

  Fragments arranged by size: 

                1216     363       8
 
EaeI y'GGCC_r

Cuts at:      0     197     318     476     515     773    1307    1587 
   Size:        197     121     158      39     258     534     280

  Fragments arranged by size: 

                 534     280     258     197     158     121      39
 
EarI CTCTTCn'nnn_

Cuts at:      0     370     610    1587 
   Size:        370     240     977

  Fragments arranged by size: 

                 977     370     240
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     385    1587 
   Size:        385    1202
 
EcoICRI GAG'CTC

Cuts at:      0    1149    1323    1587 
   Size:       1149     174     264

  Fragments arranged by size: 

                1149     264     174
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     129     394     712     732    1203    1587 
   Size:        129     265     318      20     471     384

  Fragments arranged by size: 

                 471     384     318     265     129      20
 
EcoO109I rG'GnC_Cy

Cuts at:      0      16      31     712     860    1524    1572    1587 
   Size:         16      15     681     148     664      48      15

  Fragments arranged by size: 

                 681     664     148      48      16      15      15
 
FseI GG_CCGG'CC

Cuts at:      0    1080    1587 
   Size:       1080     507
 
HaeII r_GCGC'y

Cuts at:      0     951    1587 
   Size:        951     636
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     634     666     776     808    1312    1344    1420    1452 
   Size:        634      32     110      32     504      32      76      32

Cuts at:   1452    1587 
   Size:        135

  Fragments arranged by size: 

                 634     504     135     110      76      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     558    1587 
   Size:        558    1029
 
KasI G'GCGC_C

Cuts at:      0     947    1587 
   Size:        947     640
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     821    1202    1587 
   Size:        821     381     385

  Fragments arranged by size: 

                 821     385     381
 
MscI TGG'CCA

Cuts at:      0     199     320     478     517     775    1309    1587 
   Size:        199     121     158      39     258     534     278

  Fragments arranged by size: 

                 534     278     258     199     158     121      39
 
MslI CAynn'nnrTG

Cuts at:      0     491    1163    1587 
   Size:        491     672     424

  Fragments arranged by size: 

                 672     491     424
 
MspA1I CmG'CkG

Cuts at:      0     435    1392    1416    1587 
   Size:        435     957      24     171

  Fragments arranged by size: 

                 957     435     171      24
 
NaeI GCC'GGC

Cuts at:      0     679    1078    1587 
   Size:        679     399     509

  Fragments arranged by size: 

                 679     509     399
 
NarI GG'CG_CC

Cuts at:      0     948    1587 
   Size:        948     639
 
NcoI C'CATG_G

Cuts at:      0     827    1587 
   Size:        827     760
 
NgoMIV G'CCGG_C

Cuts at:      0     677    1076    1587 
   Size:        677     399     511

  Fragments arranged by size: 

                 677     511     399
 
NspI r_CATG'y

Cuts at:      0     225     538    1587 
   Size:        225     313    1049

  Fragments arranged by size: 

                1049     313     225
 
PciI A'CATG_T

Cuts at:      0     221    1587 
   Size:        221    1366
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      65    1587 
   Size:         65    1522
 
PmlI CAC'GTG

Cuts at:      0     135    1587 
   Size:        135    1452
 
PpuMI rG'GwC_Cy

Cuts at:      0    1524    1572    1587 
   Size:       1524      48      15

  Fragments arranged by size: 

                1524      48      15
 
PspOMI G'GGCC_C

Cuts at:      0      15      31     711    1587 
   Size:         15      16     680     876

  Fragments arranged by size: 

                 876     680      16      15
 
PstI C_TGCA'G

Cuts at:      0    1196    1587 
   Size:       1196     391
 
PvuII CAG'CTG

Cuts at:      0     435    1392    1587 
   Size:        435     957     195

  Fragments arranged by size: 

                 957     435     195
 
SacI G_AGCT'C

Cuts at:      0    1151    1325    1587 
   Size:       1151     174     262

  Fragments arranged by size: 

                1151     262     174
 
ScaI AGT'ACT

Cuts at:      0     178    1587 
   Size:        178    1409
 
SfcI C'TryA_G

Cuts at:      0     738    1192    1587 
   Size:        738     454     395

  Fragments arranged by size: 

                 738     454     395
 
SfoI GGC'GCC

Cuts at:      0     949    1587 
   Size:        949     638
 
SmaI CCC'GGG

Cuts at:      0     925     945    1587 
   Size:        925      20     642

  Fragments arranged by size: 

                 925     642      20
 
SmlI C'TyrA_G

Cuts at:      0     334    1587 
   Size:        334    1253
 
SphI G_CATG'C

Cuts at:      0     538    1587 
   Size:        538    1049
 
StuI AGG'CCT

Cuts at:      0    1350    1587 
   Size:       1350     237
 
StyI C'CwwG_G

Cuts at:      0     158     827    1568    1587 
   Size:        158     669     741      19

  Fragments arranged by size: 

                 741     669     158      19
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     317    1587 
   Size:        317    1270
 
TatI w'GTAC_w

Cuts at:      0     176    1587 
   Size:        176    1411
 
Tth111I GACn'n_nGTC

Cuts at:      0     406    1587 
   Size:        406    1181
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     876    1239    1587 
   Size:        876     363     348

  Fragments arranged by size: 

                 876     363     348
 
XmaI C'CCGG_G

Cuts at:      0     923     943    1587 
   Size:        923      20     644

  Fragments arranged by size: 

                 923     644      20
 
XmnI GAAnn'nnTTC

Cuts at:      0     127    1587 
   Size:        127    1460
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI    AlwNI     ApaI    ApaLI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BglI     BlpI
 Bme1580I    BmgBI     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrFI    BssSI   BstAPI   BstEII    BstXI
    BstYI     BtgI     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I     FseI    HaeII    Hin4I   HincII     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
    PflMI     PmlI    PpuMI   PspOMI     PstI    PvuII     SacI     ScaI
     SfcI     SfoI     SmaI     SmlI     SphI     StuI     StyI    TaqII
     TatI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BcgI     BclI    BfrBI    BglII     BmrI     BmtI     BplI
     BsaI    BsaBI    BsiWI     BsmI    BspHI    BsrDI    BsrGI   BssHII
    BstBI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     EagI    EcoNI    EcoRI    EcoRV     FalI     FspI    FspAI
  HindIII     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmeI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SspI     SwaI     TstI     XbaI     XhoI     ZraI
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