Restriction map for pF1KB8297
##################################
# NO CUT ENZYMES (  163 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvrII       BalI        BamHI       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BfaI        BfrI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRI       
EcoRV       EcoT14I     EcoT22I     FseI        HaeII       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     MaeI        MaeII       MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RsrII       SacI        SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tru9I       
Tsp509I     Tth111I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AcyI          178  GACGCC               G[GA]^CG[CT]C
AlwNI          73  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaI           28  CCCGAG               C^[CT]CG[GA]G
AviII         413  TGCGCA               TGC^GCA
BanII         430  GGGCTC               G[GA]GC[CT]^C
BbsI            6  GAAGAC               GAAGAC(2/6)
BclI          404  TGATCA               T^GATCA
BcnI          246  CCGGG                CC^[GC]GG
BglI          113  GCCCCAGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         458  AGATCT               A^GATCT
BpuAI           6  GAAGAC               GAAGAC(2/6)
BsaHI         178  GACGCC               G[GA]^CG[CT]C
BsoBI          28  CCCGAG               C^[CT]CG[GA]G
BspHI         407  TCATGA               T^CATGA
BsrI          305  ACTGG                ACTGG(1/-1)
BstEII        270  GGTCACC              G^GT[ACGT]ACC
BstPI         270  GGTCACC              G^GT[ACGT]ACC
BstYI         458  AGATCT               [GA]^GATC[CT]
DdeI           59  CTGAG                C^T[ACGT]AG
DraII          41  AGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI          279  CCGCGG               C^C[GA][CT]GG
EcoO109I       41  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       270  GGTCACC              G^GT[ACGT]ACC
FauI          278  CCCGC                CCCGC(4/6)
FbaI          404  TGATCA               T^GATCA
FspI          413  TGCGCA               TGC^GCA
HapII         246  CCGG                 C^CGG
HgaI          178  GACGC                GACGC(5/10)
Hin1I         178  GACGCC               G[GA]^CG[CT]C
HinfI         344  GAATC                G^A[ACGT]TC
HpaII         246  CCGG                 C^CGG
HphI          272  TCACC                GGTGA(8/7)
KspI          279  CCGCGG               CCGC^GG
MflI          458  AGATCT               [GA]^GATC[CT]
MspI          246  CCGG                 C^CGG
NciI          246  CCGGG                CC^[GC]GG
NspI          563  ACATGC               [GA]CATG^[CT]
PpuMI          41  AGGACCT              [GA]G^G[AT]CC[CT]
PstI          553  CTGCAG               CTGCA^G
RcaI          407  TCATGA               T^CATGA
SacII         279  CCGCGG               CCGC^GG
SfcI          553  CTGCAG               C^T[GA][CT]AG
TfiI          344  GAATC                G^A[AT]TC
XcmI           11  CCAACAGCAAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         458  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AfaI           90  GTAC                 GT^AC
              321  GTAC                
AvaII          42  GGACC                G^G[AT]CC
              474  GGACC               
BanI          232  GGCACC               G^G[CT][GA]CC
              397  GGCACC              
BmyI          396  GGGCAC               G[AGT]GC[ACT]^C
              430  GGGCTC              
BsaJI          29  CCGAGG               C^C[ACGT][ACGT]GG
              279  CCGCGG              
BseRI         136  GAGGAG               GAGGAG(10/8)
              505  GAGGAG              
BsiYI         126  CCTCAACCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              477  CCAGGATAAGG         
BslI          126  CCTCAACCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              477  CCAGGATAAGG         
Bsp1286I      396  GGGCAC               G[AGT]GC[ACT]^C
              430  GGGCTC              
BstF5I        123  CATCC                GGATG(2/0)
              536  CATCC               
FokI          123  CATCC                GGATG(9/13)
              536  CATCC               
HaeIII         24  GGCC                 GG^CC
              309  GGCC                
MspA1I        117  CAGCGG               C[AC]G^C[GT]G
              279  CCGCGG              
RsaI           90  GTAC                 GT^AC
              321  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AccII       AciI        AluI        BbvI        BpmI        
BssKI       BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DpnII       EcoRII      Fnu4HI      HhaI        
HinP1I      ItaI        MaeIII      MboI        MboII       
MnlI        MvaI        MvnI        MwoI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        TseI        Tsp45I      TspRI       
TthHB8I     
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