Restriction map for
pF1KB8961
(Linear) (Six-base) MAPSORT of: KIBB8961.seq Check: 3910 from: 1 to: 1026
KIBB8961.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 109 1026
Size: 109 917
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 1026
Size: 754 272
AfeI AGC'GCT
Cuts at: 0 835 1026
Size: 835 191
AflIII A'CryG_T
Cuts at: 0 890 1026
Size: 890 136
ApoI r'AATT_y
Cuts at: 0 877 1026
Size: 877 149
AvaI C'yCGr_G
Cuts at: 0 348 461 557 583 902 1026
Size: 348 113 96 26 319 124
Fragments arranged by size:
348 319 124 113 96 26
BamHI G'GATC_C
Cuts at: 0 806 1026
Size: 806 220
BanI G'GyrC_C
Cuts at: 0 13 213 434 1026
Size: 13 200 221 592
Fragments arranged by size:
592 221 200 13
BanII G_rGCy'C
Cuts at: 0 305 695 1026
Size: 305 390 331
Fragments arranged by size:
390 331 305
BbeI G_GCGC'C
Cuts at: 0 438 1026
Size: 438 588
BbvCI CC'TCA_GC
Cuts at: 0 19 696 1026
Size: 19 677 330
Fragments arranged by size:
677 330 19
BglI GCCn_nnn'nGGC
Cuts at: 0 56 93 419 1026
Size: 56 37 326 607
Fragments arranged by size:
607 326 56 37
Bme1580I G_kGCm'C
Cuts at: 0 218 1026
Size: 218 808
BmrI ACTGGGnnnn_n'
Cuts at: 0 680 1026
Size: 680 346
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 177 1026
Size: 177 849
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 1026
Size: 889 137
Bpu10I CC'TnA_GC
Cuts at: 0 19 696 1026
Size: 19 677 330
Fragments arranged by size:
677 330 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 883 1026
Size: 883 143
BsaAI yAC'GTr
Cuts at: 0 33 315 1026
Size: 33 282 711
Fragments arranged by size:
711 282 33
BsaHI Gr'CG_yC
Cuts at: 0 435 1026
Size: 435 591
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 304 334 802 832 1026
Size: 304 30 468 30 194
Fragments arranged by size:
468 304 194 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 492 495 498 516 519 754 757 1026
Size: 492 3 3 18 3 235 3 269
Fragments arranged by size:
492 269 235 18 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 221 403 415 423 1026
Size: 221 182 12 8 603
Fragments arranged by size:
603 221 182 12 8
BsiEI CG_ry'CG
Cuts at: 0 140 188 309 1026
Size: 140 48 121 717
Fragments arranged by size:
717 140 121 48
BsiHKAI G_wGCw'C
Cuts at: 0 550 1026
Size: 550 476
BsiWI C'GTAC_G
Cuts at: 0 263 1026
Size: 263 763
BsmI GAATG_Cn'
Cuts at: 0 753 1026
Size: 753 273
Bsp1286I G_dGCh'C
Cuts at: 0 218 305 550 695 1026
Size: 218 87 245 145 331
Fragments arranged by size:
331 245 218 145 87
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 109 1026
Size: 109 917
BsrBI CCG'CTC
Cuts at: 0 248 466 1026
Size: 248 218 560
Fragments arranged by size:
560 248 218
BsrFI r'CCGG_y
Cuts at: 0 110 908 1026
Size: 110 798 118
Fragments arranged by size:
798 118 110
BssHII G'CGCG_C
Cuts at: 0 76 101 1026
Size: 76 25 925
Fragments arranged by size:
925 76 25
BssSI C'ACGA_G
Cuts at: 0 162 201 591 1026
Size: 162 39 390 435
Fragments arranged by size:
435 390 162 39
BstYI r'GATC_y
Cuts at: 0 806 1026
Size: 806 220
BtgI C'CryG_G
Cuts at: 0 209 396 482 551 1026
Size: 209 187 86 69 475
Fragments arranged by size:
475 209 187 86 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 256 1026
Size: 256 770
EaeI y'GGCC_r
Cuts at: 0 137 185 284 306 923 1026
Size: 137 48 99 22 617 103
Fragments arranged by size:
617 137 103 99 48 22
EagI C'GGCC_G
Cuts at: 0 137 185 306 1026
Size: 137 48 121 720
Fragments arranged by size:
720 137 121 48
EarI CTCTTCn'nnn_
Cuts at: 0 889 1026
Size: 889 137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 889 1026
Size: 754 135 137
Fragments arranged by size:
754 137 135
EcoNI CCTnn'n_nnAGG
Cuts at: 0 650 1026
Size: 650 376
EcoRI G'AATT_C
Cuts at: 0 877 1026
Size: 877 149
FseI GG_CCGG'CC
Cuts at: 0 114 1026
Size: 114 912
HaeII r_GCGC'y
Cuts at: 0 438 837 915 1026
Size: 438 399 78 111
Fragments arranged by size:
438 399 111 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 798 830 1026
Size: 798 32 196
Fragments arranged by size:
798 196 32
KasI G'GCGC_C
Cuts at: 0 434 1026
Size: 434 592
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 1026
Size: 668 358
MscI TGG'CCA
Cuts at: 0 925 1026
Size: 925 101
MspA1I CmG'CkG
Cuts at: 0 211 235 336 398 419 578 625 1026
Size: 211 24 101 62 21 159 47 401
Fragments arranged by size:
401 211 159 101 62 47 24 21
NaeI GCC'GGC
Cuts at: 0 112 1026
Size: 112 914
NarI GG'CG_CC
Cuts at: 0 435 1026
Size: 435 591
NgoMIV G'CCGG_C
Cuts at: 0 110 1026
Size: 110 916
NspI r_CATG'y
Cuts at: 0 894 1026
Size: 894 132
PasI CC'CwG_GG
Cuts at: 0 567 1026
Size: 567 459
PciI A'CATG_T
Cuts at: 0 890 1026
Size: 890 136
PfoI T'CCnGG_A
Cuts at: 0 322 1026
Size: 322 704
PstI C_TGCA'G
Cuts at: 0 120 611 1026
Size: 120 491 415
Fragments arranged by size:
491 415 120
RsrII CG'GwC_CG
Cuts at: 0 388 1026
Size: 388 638
SacII CC_GC'GG
Cuts at: 0 212 399 1026
Size: 212 187 627
Fragments arranged by size:
627 212 187
SbfI CC_TGCA'GG
Cuts at: 0 120 611 1026
Size: 120 491 415
Fragments arranged by size:
491 415 120
SfcI C'TryA_G
Cuts at: 0 116 607 1026
Size: 116 491 419
Fragments arranged by size:
491 419 116
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 93 419 1026
Size: 93 326 607
Fragments arranged by size:
607 326 93
SfoI GGC'GCC
Cuts at: 0 436 1026
Size: 436 590
SmaI CCC'GGG
Cuts at: 0 350 559 904 1026
Size: 350 209 345 122
Fragments arranged by size:
350 345 209 122
SmlI C'TyrA_G
Cuts at: 0 862 1026
Size: 862 164
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 802 834 1026
Size: 802 32 192
Fragments arranged by size:
802 192 32
XmaI C'CCGG_G
Cuts at: 0 348 557 902 1026
Size: 348 209 345 124
Fragments arranged by size:
348 345 209 124
XmnI GAAnn'nnTTC
Cuts at: 0 877 1026
Size: 877 149
Enzymes that do cut:
AarI AcuI AfeI AflIII ApoI AvaI BamHI BanI
BanII BbeI BbvCI BglI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspMI BsrBI BsrFI BssHII
BssSI BstYI BtgI BtgZI EaeI EagI EarI Eco57MI
EcoNI EcoRI FseI HaeII Hin4I KasI MmeI MscI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PfoI
PstI RsrII SacII SbfI SfcI SfiI SfoI SmaI
SmlI TstI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI BsaI BsaBI BsaWI BsgI BsmBI
BspEI BspHI BsrDI BsrGI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI
EciI EcoICRI EcoO109I EcoRV FalI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MslI NcoI NdeI
NheI NotI NruI NsiI PacI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII SacI SalI SanDI SapI ScaI SexAI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TatI Tth111I XbaI XcmI XhoI ZraI