Restriction map for
pF1KB7326
(Circular) (Six-base) MAPSORT of: pF1KB7326.seq Check: 8485 from: 1 to: 6758
pF1KB7326 6758 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 364 3705 364
Size: 3341 3417
AccI GT'mk_AC
Cuts at: 128 3796 128
Size: 3668 3090
Acc65I G'GTAC_C
Cuts at: 3774 3774
Size: 6758
AclI AA'CG_TT
Cuts at: 6598 6598
Size: 6758
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3255 4583 5015 5762 6030 3255
Size: 1328 432 747 268 3983
Fragments arranged by size:
3983 1328 747 432 268
AflIII A'CryG_T
Cuts at: 1250 2358 4110 5214 6191 1250
Size: 1108 1752 1104 977 1817
Fragments arranged by size:
1817 1752 1108 1104 977
AgeI A'CCGG_T
Cuts at: 5146 5146
Size: 6758
AhdI GACnn_n'nnGTC
Cuts at: 1143 1254 1143
Size: 111 6647
AleI CACnn'nnGTG
Cuts at: 3013 3409 3013
Size: 396 6362
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 365 544 2628 365
Size: 179 2084 4495
Fragments arranged by size:
4495 2084 179
AlwNI CAG_nnn'CTG
Cuts at: 1939 2154 4063 5630 1939
Size: 215 1909 1567 3067
Fragments arranged by size:
3067 1909 1567 215
ApaLI G'TGCA_C
Cuts at: 1909 5528 1909
Size: 3619 3139
ApoI r'AATT_y
Cuts at: 261 785 976 1597 1767 1848 3762 6111 6367
Size: 524 191 621 170 81 1914 2349 256
Cuts at: 6367 261
Size: 652
Fragments arranged by size:
2349 1914 652 621 524 256 191 170
81
AseI AT'TA_AT
Cuts at: 20 2537 3169 20
Size: 2517 632 3609
Fragments arranged by size:
3609 2517 632
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6758
AvaI C'yCGr_G
Cuts at: 320 2694 3778 320
Size: 2374 1084 3300
Fragments arranged by size:
3300 2374 1084
BamHI G'GATC_C
Cuts at: 1101 3783 1101
Size: 2682 4076
BanI G'GyrC_C
Cuts at: 423 2157 3093 3774 4188 4436 4471 423
Size: 1734 936 681 414 248 35 2710
Fragments arranged by size:
2710 1734 936 681 414 248 35
BanII G_rGCy'C
Cuts at: 1209 3772 4802 1209
Size: 2563 1030 3165
Fragments arranged by size:
3165 2563 1030
BbeI G_GCGC'C
Cuts at: 4192 4440 4192
Size: 248 6510
BbsI GAAGACnn'nnnn_
Cuts at: 3339 3339
Size: 6758
BbvCI CC'TCA_GC
Cuts at: 2333 2372 2333
Size: 39 6719
BciVI GTATCCnnnnn_n'
Cuts at: 3057 3752 4656 5417 6758 3057
Size: 695 904 761 1341 3057
Fragments arranged by size:
3057 1341 904 761 695
BclI T'GATC_A
Cuts at: 1323 4278 1323
Size: 2955 3803
BglI GCCn_nnn'nGGC
Cuts at: 2166 3424 2166
Size: 1258 5500
BglII A'GATC_T
Cuts at: 4273 6310 4273
Size: 2037 4721
BlpI GC'TnA_GC
Cuts at: 3870 3870
Size: 6758
Bme1580I G_kGCm'C
Cuts at: 1913 3329 4383 4476 5532 1913
Size: 1416 1054 93 1056 3139
Fragments arranged by size:
3139 1416 1056 1054 93
BmgBI CAC'GTC
Cuts at: 2129 2129
Size: 6758
BmrI ACTGGGnnnn_n'
Cuts at: 2256 3572 3595 3970 4051 4386 6530 2256
Size: 1316 23 375 81 335 2144 2484
Fragments arranged by size:
2484 2144 1316 375 335 81 23
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5125 5125
Size: 6758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 659 1193 1637 659
Size: 534 444 5780
Fragments arranged by size:
5780 534 444
Bpu10I CC'TnA_GC
Cuts at: 398 2333 2372 398
Size: 1935 39 4784
Fragments arranged by size:
4784 1935 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1060 1334 3519 3934 4813 5305 5603 1060
Size: 274 2185 415 879 492 298 2215
Fragments arranged by size:
2215 2185 879 492 415 298 274
BsaI GGTCTCn'nnnn_
Cuts at: 1654 2898 6462 1654
Size: 1244 3564 1950
Fragments arranged by size:
3564 1950 1244
BsaAI yAC'GTr
Cuts at: 417 4111 417
Size: 3694 3064
BsaBI GATnn'nnATC
Cuts at: 6 4272 6309 6
Size: 4266 2037 455
Fragments arranged by size:
4266 2037 455
BsaHI Gr'CG_yC
Cuts at: 4189 4437 5139 6635 4189
Size: 248 702 1496 4312
Fragments arranged by size:
4312 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3947 4468 5146 5420 5567 5978 3947
Size: 521 678 274 147 411 4727
Fragments arranged by size:
4727 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 517 2862 517
Size: 2345 4413
BseYI C'CCAG_C
Cuts at: 473 1992 2082 4183 4941 5518 6014 473
Size: 1519 90 2101 758 577 496 1217
Fragments arranged by size:
2101 1519 1217 758 577 496 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 1016 2110 3482 439
Size: 577 1094 1372 3715
Fragments arranged by size:
3715 1372 1094 577
BsiEI CG_ry'CG
Cuts at: 104 4300 4346 5554 104
Size: 4196 46 1208 1308
Fragments arranged by size:
4196 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1209 1913 2812 3010 3772 4550 4740 5532 1209
Size: 704 899 198 762 778 190 792 2435
Fragments arranged by size:
2435 899 792 778 762 704 198 190
BsmI GAATG_Cn'
Cuts at: 901 3247 901
Size: 2346 4412
Bsp1286I G_dGCh'C
Cuts at: 1209 1913 2812 3010 3329 3772 4383 4476 4550
Size: 704 899 198 319 443 611 93 74
Cuts at: 4550 4740 4802 5532 1209
Size: 190 62 730 2435
Fragments arranged by size:
2435 899 730 704 611 443 319 198
190 93 74 62
BspEI T'CCGG_A
Cuts at: 5978 5978
Size: 6758
BspHI T'CATG_A
Cuts at: 3027 5934 3027
Size: 2907 3851
BspMI ACCTGCnnnn'nnnn_
Cuts at: 364 3705 3808 4022 4324 4705 364
Size: 3341 103 214 302 381 2417
Fragments arranged by size:
3341 2417 381 302 214 103
BsrBI CCG'CTC
Cuts at: 50 1299 5050 5104 6588 6753 50
Size: 1249 3751 54 1484 165 55
Fragments arranged by size:
3751 1484 1249 165 55 54
BsrDI GCAATG_nn'
Cuts at: 337 533 569 3683 4670 337
Size: 196 36 3114 987 2425
Fragments arranged by size:
3114 2425 987 196 36
BsrFI r'CCGG_y
Cuts at: 4756 4937 5146 4756
Size: 181 209 6368
Fragments arranged by size:
6368 209 181
BssSI C'ACGA_G
Cuts at: 5029 5387 5029
Size: 358 6400
BstAPI GCAn_nnn'nTGC
Cuts at: 1939 3415 3697 4135 1939
Size: 1476 282 438 4562
Fragments arranged by size:
4562 1476 438 282
BstBI TT'CG_AA
Cuts at: 5119 5119
Size: 6758
BstXI CCAn_nnnn'nTGG
Cuts at: 481 3427 3580 481
Size: 2946 153 3659
Fragments arranged by size:
3659 2946 153
BstYI r'GATC_y
Cuts at: 1 1101 2102 3292 3783 4250 4273 4608 4854
Size: 1100 1001 1190 491 467 23 335 246
Cuts at: 4854 5866 5952 5964 6310 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1190 1100 1012 1001 491 467 449 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2874 2874
Size: 6758
BtgI C'CryG_G
Cuts at: 107 229 1914 107
Size: 122 1685 4951
Fragments arranged by size:
4951 1685 122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 492 4708 4889 5052 492
Size: 4216 181 163 2198
Fragments arranged by size:
4216 2198 181 163
BtsI GCAGTG_nn'
Cuts at: 368 1028 2711 2733 3221 3416 4001 368
Size: 660 1683 22 488 195 585 3125
Fragments arranged by size:
3125 1683 660 585 488 195 22
DraI TTT'AAA
Cuts at: 3757 3757
Size: 6758
DrdI GACnn_nn'nnGTC
Cuts at: 4464 5322 4464
Size: 858 5900
EaeI y'GGCC_r
Cuts at: 732 1025 1148 1294 4343 4517 4908 4935 6280
Size: 293 123 146 3049 174 391 27 1345
Cuts at: 6280 732
Size: 1210
Fragments arranged by size:
3049 1345 1210 391 293 174 146 123
27
EagI C'GGCC_G
Cuts at: 4343 4343
Size: 6758
EarI CTCTTCn'nnn_
Cuts at: 1451 1587 2306 2445 2738 2921 2982 4781 4991
Size: 136 719 139 293 183 61 1799 210
Cuts at: 4991 1451
Size: 3218
Fragments arranged by size:
3218 1799 719 293 210 183 139 136
61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4966 5276 5422 6568 4966
Size: 310 146 1146 5156
Fragments arranged by size:
5156 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1207 3770 1207
Size: 2563 4195
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 659 1193 1637 3255 4583 5015 5762 6030 659
Size: 534 444 1618 1328 432 747 268 1387
Fragments arranged by size:
1618 1387 1328 747 534 444 432 268
EcoO109I rG'GnC_Cy
Cuts at: 469 2079 3897 469
Size: 1610 1818 3330
Fragments arranged by size:
3330 1818 1610
EcoRI G'AATT_C
Cuts at: 3762 3762
Size: 6758
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4777 4777
Size: 6758
FspI TGC'GCA
Cuts at: 4539 4539
Size: 6758
HaeII r_GCGC'y
Cuts at: 4192 4440 5462 6441 4192
Size: 248 1022 979 4509
Fragments arranged by size:
4509 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 532 564 1129 1161 1240 1272 1283 1315 1971
Size: 32 565 32 79 32 11 32 656
Cuts at: 1971 2003 2750 2782 4600 4630 4632 4662 532
Size: 32 747 32 1818 30 2 30 2628
Fragments arranged by size:
2628 1818 747 656 565 79 32 32
32 32 32 32 30 30 11 2
HincII GTy'rAC
Cuts at: 3797 3797
Size: 6758
HindIII A'AGCT_T
Cuts at: 2798 3341 2798
Size: 543 6215
KasI G'GCGC_C
Cuts at: 4188 4436 4188
Size: 248 6510
KpnI G_GTAC'C
Cuts at: 3778 3778
Size: 6758
MluI A'CGCG_T
Cuts at: 6191 6191
Size: 6758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 488 1417 2130 2455 2989 3580 3748 5429 5613
Size: 929 713 325 534 591 168 1681 184
Cuts at: 5613 488
Size: 1633
Fragments arranged by size:
1681 1633 929 713 591 534 325 184
168
MscI TGG'CCA
Cuts at: 734 1027 4519 734
Size: 293 3492 2973
Fragments arranged by size:
3492 2973 293
MslI CAynn'nnrTG
Cuts at: 1249 1263 1546 3013 3409 3578 4747 4874 1249
Size: 14 283 1467 396 169 1169 127 3133
Fragments arranged by size:
3133 1467 1169 396 283 169 127 14
MspA1I CmG'CkG
Cuts at: 1657 2086 2667 3076 3869 4183 4543 5556 5801
Size: 429 581 409 793 314 360 1013 245
Cuts at: 5801 1657
Size: 2614
Fragments arranged by size:
2614 1013 793 581 429 409 360 314
245
NaeI GCC'GGC
Cuts at: 4939 4939
Size: 6758
NarI GG'CG_CC
Cuts at: 4189 4437 4189
Size: 248 6510
NcoI C'CATG_G
Cuts at: 107 1914 107
Size: 1807 4951
NdeI CA'TA_TG
Cuts at: 138 3137 138
Size: 2999 3759
NgoMIV G'CCGG_C
Cuts at: 4937 4937
Size: 6758
NspI r_CATG'y
Cuts at: 1254 1537 2362 3418 3811 4311 5218 1254
Size: 283 825 1056 393 500 907 2794
Fragments arranged by size:
2794 1056 907 825 500 393 283
PciI A'CATG_T
Cuts at: 1250 2358 5214 1250
Size: 1108 2856 2794
Fragments arranged by size:
2856 2794 1108
PfoI T'CCnGG_A
Cuts at: 212 212
Size: 6758
PmeI GTTT'AAAC
Cuts at: 3757 3757
Size: 6758
PmlI CAC'GTG
Cuts at: 417 417
Size: 6758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5897 5929 5897
Size: 32 6726
PsiI TTA'TAA
Cuts at: 2279 3563 2279
Size: 1284 5474
PspXI vC'TCGA_Gb
Cuts at: 320 320
Size: 6758
PstI C_TGCA'G
Cuts at: 375 1931 2706 3216 3805 4490 375
Size: 1556 775 510 589 685 2643
Fragments arranged by size:
2643 1556 775 685 589 510
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6758
PvuII CAG'CTG
Cuts at: 1657 2086 3076 4183 4543 1657
Size: 429 990 1107 360 3872
Fragments arranged by size:
3872 1107 990 429 360
RsrII CG'GwC_CG
Cuts at: 4953 4953
Size: 6758
SacI G_AGCT'C
Cuts at: 1209 3772 1209
Size: 2563 4195
SalI G'TCGA_C
Cuts at: 3795 3795
Size: 6758
SapI GCTCTTCn'nnn_
Cuts at: 1451 2921 4781 4991 1451
Size: 1470 1860 210 3218
Fragments arranged by size:
3218 1860 1470 210
SbfI CC_TGCA'GG
Cuts at: 3805 3805
Size: 6758
ScaI AGT'ACT
Cuts at: 855 1830 2856 6275 855
Size: 975 1026 3419 1338
Fragments arranged by size:
3419 1338 1026 975
SfcI C'TryA_G
Cuts at: 33 371 1200 1927 2098 2702 3212 3801 4486
Size: 338 829 727 171 604 510 589 685
Cuts at: 4486 5479 5670 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 829 727 685 604 589 510
338 191 171
SfoI GGC'GCC
Cuts at: 4190 4438 4190
Size: 248 6510
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6758
SmaI CCC'GGG
Cuts at: 3780 3780
Size: 6758
SmlI C'TyrA_G
Cuts at: 320 1075 1349 3534 3913 4828 5320 5582 320
Size: 755 274 2185 379 915 492 262 1496
Fragments arranged by size:
2185 1496 915 755 492 379 274 262
SpeI A'CTAG_T
Cuts at: 67 3355 67
Size: 3288 3470
SphI G_CATG'C
Cuts at: 3418 3811 4311 3418
Size: 393 500 5865
Fragments arranged by size:
5865 500 393
SspI AAT'ATT
Cuts at: 1089 2820 1089
Size: 1731 5027
StyI C'CwwG_G
Cuts at: 107 1313 1914 3892 4246 107
Size: 1206 601 1978 354 2619
Fragments arranged by size:
2619 1978 1206 601 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2922 4341 4474 4933 5142 2922
Size: 1419 133 459 209 4538
Fragments arranged by size:
4538 1419 459 209 133
TatI w'GTAC_w
Cuts at: 660 853 1828 2854 3653 6273 660
Size: 193 975 1026 799 2620 1145
Fragments arranged by size:
2620 1145 1026 975 799 193
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 575 607 2087 2119 6275 6307 575
Size: 32 1480 32 4156 32 1026
Fragments arranged by size:
4156 1480 1026 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2130 4555 2130
Size: 2425 4333
XbaI T'CTAG_A
Cuts at: 1698 2787 3789 1698
Size: 1089 1002 4667
Fragments arranged by size:
4667 1089 1002
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4253 4253
Size: 6758
XhoI C'TCGA_G
Cuts at: 320 320
Size: 6758
XmaI C'CCGG_G
Cuts at: 3778 3778
Size: 6758
XmnI GAAnn'nnTTC
Cuts at: 899 1283 899
Size: 384 6374
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PfoI PmeI PmlI PpiI PsiI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AloI ApaI AscI AvrII BaeI
BcgI BfrBI BmtI BsaXI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NheI NotI NruI NsiI PacI
PasI PflMI PpuMI PshAI PspOMI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI ZraI