Restriction map for pF1KB7665
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     AspEI       AspI        
AvaII       AviII       AvrII       BalI        BbeI        
BbrPI       BcgI        BciVI       BclI        BcnI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaWI       BseAI       BsiEI       
BsiWI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRV       EcoT22I     FbaI        FseI        FspI        
HapII       HindIII     HpaI        HpaII       HphI        
KasI        Ksp632I     MaeIII      MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MspI        MunI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RsrII       SalI        
SapI        SbfI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SwaI        Tru9I       Tsp45I      Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I        404  GGTACC               G^GTACC
AcsI          111  GAATTC               [GA]^AATT[CT]
AcyI          176  GGCGTC               G[GA]^CG[CT]C
ApoI          111  GAATTC               [GA]^AATT[CT]
Asp718I       404  GGTACC               G^GTACC
AspHI         411  GAGCTC               G[AT]GC[AT]^C
AvaI           78  CTCGGG               C^[CT]CG[GA]G
BamHI         438  GGATCC               G^GATCC
BanI          404  GGTACC               G^G[CT][GA]CC
BanII         411  GAGCTC               G[GA]GC[CT]^C
BbsI          215  GAAGAC               GAAGAC(2/6)
BbvCI         943  CCTCAGC              CCTCAGC(-5/-2)
BfaI           92  CTAG                 C^TAG
BglI          811  GCCACCGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          411  GAGCTC               G[AGT]GC[ACT]^C
BpmI          589  CTCCAG               CTGGAG(16/14)
BpuAI         215  GAAGAC               GAAGAC(2/6)
BsaHI         176  GGCGTC               G[GA]^CG[CT]C
BsaI           46  GAGACC               GGTCTC(1/5)
BsgI          821  CTGCAC               GTGCAG(16/14)
BsiHKAI       411  GAGCTC               G[AT]GC[AT]^C
BsmBI          19  GAGACG               CGTCTC(1/5)
BsoBI          78  CTCGGG               C^[CT]CG[GA]G
Bsp1286I      411  GAGCTC               G[AGT]GC[ACT]^C
BspHI         362  TCATGA               T^CATGA
BspMI         572  ACCTGC               ACCTGC(4/8)
BsrBI         535  CCGCTC               CCGCTC(-3/-3)
BstXI          50  CCATCGACGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoRI         111  GAATTC               G^AATTC
HaeII           5  AGCGCC               [GA]GCGC^[CT]
Hin1I         176  GGCGTC               G[GA]^CG[CT]C
HincII        618  GTCAAC               GT[CT]^[GA]AC
HindII        618  GTCAAC               GT[CT]^[GA]AC
KpnI          404  GGTACC               GGTAC^C
MaeI           92  CTAG                 C^TAG
MslI           51  CATCGACGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
RcaI          362  TCATGA               T^CATGA
SacI          411  GAGCTC               GAGCT^C
ScaI           88  AGTACT               AGT^ACT
SspI          273  AATATT               AAT^ATT
StuI          423  AGGCCT               AGG^CCT
TaqI           53  TCGA                 T^CGA
TthHB8I        53  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AfaI           89  GTAC                 GT^AC
              405  GTAC                
BsmFI         991  GGGAC                GGGAC(10/14)
              774  GTCCC               
BsmI          716  GAATGC               GAATGC(1/-1)
              853  GCATTC              
BsrI          551  ACTGG                ACTGG(1/-1)
              938  CCAGT               
BssKI         426  CCTGG                ^CC[ACGT]GG
              986  CCTGG               
BstNI         426  CCTGG                CC^[AT]GG
              986  CCTGG               
BstYI         247  GGATCT               [GA]^GATC[CT]
              438  GGATCC              
Cfr13I        519  GGCCC                G^G[ACGT]CC
              554  GGGCC               
EcoRII        426  CCTGG                ^CC[AT]GG
              986  CCTGG               
EcoT14I       353  CCATGG               C^C[AT][AT]GG
              515  CCATGG              
KspI          815  CCGCGG               CCGC^GG
              893  CCGCGG              
MboII         215  GAAGA                GAAGA(8/7)
              300  GAAGA               
MflI          247  GGATCT               [GA]^GATC[CT]
              438  GGATCC              
MvaI          426  CCTGG                CC^[AT]GG
              986  CCTGG               
NcoI          353  CCATGG               C^CATGG
              515  CCATGG              
NlaIV         404  GGTACC               GG[ACGT]^[ACGT]CC
              438  GGATCC              
PleI          430  GACTC                GAGTC(4/5)
              616  GAGTC               
RsaI           89  GTAC                 GT^AC
              405  GTAC                
SacII         815  CCGCGG               CCGC^GG
              893  CCGCGG              
Sau96I        519  GGCCC                G^G[ACGT]CC
              554  GGGCC               
ScrFI         426  CCTGG                CC^[ACGT]GG
              986  CCTGG               
SfaNI         303  GATGC                GCATC(5/9)
              546  GCATC               
StyI          353  CCATGG               C^C[AT][AT]GG
              515  CCATGG              
TfiI          374  GATTC                G^A[AT]TC
              703  GATTC               
Tsp509I        73  AATT                 ^AATT
              112  AATT                
XhoII         247  GGATCT               [GA]^GATC[CT]
              438  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        BbvI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BstF5I      BstUI       Cac8I       CfoI        DdeI        
DpnII       DsaI        EaeI        FauI        Fnu4HI      
FokI        HaeIII      HgaI        HhaI        HinP1I      
HinfI       ItaI        MaeII       MboI        MnlI        
MspA1I      MvnI        MwoI        NdeII       NlaIII      
Sau3AI      TseI        TspRI       
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