Restriction map for pF1KB7665
 (Linear) (Six-base) MAPSORT of: KIBB7665.seq  Check: 2262  from: 1  to: 1011



KIBB7665.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     581    1011 
   Size:        581     430
 
Acc65I G'GTAC_C

Cuts at:      0     404    1011 
   Size:        404     607
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     629    1011 
   Size:        292     337     382

  Fragments arranged by size: 

                 382     337     292
 
ApoI r'AATT_y

Cuts at:      0     111    1011 
   Size:        111     900
 
AvaI C'yCGr_G

Cuts at:      0      78    1011 
   Size:         78     933
 
BamHI G'GATC_C

Cuts at:      0     438    1011 
   Size:        438     573
 
BanI G'GyrC_C

Cuts at:      0     404    1011 
   Size:        404     607
 
BanII G_rGCy'C

Cuts at:      0     415    1011 
   Size:        415     596
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     222    1011 
   Size:        222     789
 
BbvCI CC'TCA_GC

Cuts at:      0     944    1011 
   Size:        944      67
 
BglI GCCn_nnn'nGGC

Cuts at:      0     817    1011 
   Size:        817     194
 
BmgBI CAC'GTC

Cuts at:      0      57     935    1011 
   Size:         57     878      76

  Fragments arranged by size: 

                 878      76      57
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     561    1011 
   Size:        561     450
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      42    1011 
   Size:         42     969
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     574    1011 
   Size:        574     437
 
Bpu10I CC'TnA_GC

Cuts at:      0     409     944    1011 
   Size:        409     535      67

  Fragments arranged by size: 

                 535     409      67
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      40    1011 
   Size:         40     971
 
BsaHI Gr'CG_yC

Cuts at:      0     177    1011 
   Size:        177     834
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     397     427    1011 
   Size:        397      30     584

  Fragments arranged by size: 

                 584     397      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      31     423     667    1011 
   Size:         31     392     244     344

  Fragments arranged by size: 

                 392     344     244      31
 
BseYI C'CCAG_C

Cuts at:      0     485    1011 
   Size:        485     526
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     806    1011 
   Size:        806     205
 
BsiHKAI G_wGCw'C

Cuts at:      0     415    1011 
   Size:        415     596
 
BsmI GAATG_Cn'

Cuts at:      0     722     853    1011 
   Size:        722     131     158

  Fragments arranged by size: 

                 722     158     131
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      13    1011 
   Size:         13     998
 
Bsp1286I G_dGCh'C

Cuts at:      0     415    1011 
   Size:        415     596
 
BspHI T'CATG_A

Cuts at:      0     362    1011 
   Size:        362     649
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     581    1011 
   Size:        581     430
 
BsrBI CCG'CTC

Cuts at:      0     537    1011 
   Size:        537     474
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      57    1011 
   Size:         57     954
 
BstYI r'GATC_y

Cuts at:      0     247     438    1011 
   Size:        247     191     573

  Fragments arranged by size: 

                 573     247     191
 
BtgI C'CryG_G

Cuts at:      0     353     416     515     663     725     815     893    1011 
   Size:        353      63      99     148      62      90      78     118

  Fragments arranged by size: 

                 353     148     118      99      90      78      63      62
            
 
BtsI GCAGTG_nn'

Cuts at:      0     893     963    1011 
   Size:        893      70      48

  Fragments arranged by size: 

                 893      70      48
 
EaeI y'GGCC_r

Cuts at:      0     500     683     890     896    1011 
   Size:        500     183     207       6     115

  Fragments arranged by size: 

                 500     207     183     115       6
 
EcoICRI GAG'CTC

Cuts at:      0     413    1011 
   Size:        413     598
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     574     629    1011 
   Size:        292     282      55     382

  Fragments arranged by size: 

                 382     292     282      55
 
EcoRI G'AATT_C

Cuts at:      0     111    1011 
   Size:        111     900
 
HaeII r_GCGC'y

Cuts at:      0       9    1011 
   Size:          9    1002
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     276     308    1011 
   Size:        276      32     703

  Fragments arranged by size: 

                 703     276      32
 
HincII GTy'rAC

Cuts at:      0     620    1011 
   Size:        620     391
 
KpnI G_GTAC'C

Cuts at:      0     408    1011 
   Size:        408     603
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     981    1011 
   Size:        142     839      30

  Fragments arranged by size: 

                 839     142      30
 
MslI CAynn'nnrTG

Cuts at:      0      55    1011 
   Size:         55     956
 
MspA1I CmG'CkG

Cuts at:      0     444     489     817     895    1011 
   Size:        444      45     328      78     116

  Fragments arranged by size: 

                 444     328     116      78      45
 
NcoI C'CATG_G

Cuts at:      0     353     515    1011 
   Size:        353     162     496

  Fragments arranged by size: 

                 496     353     162
 
PasI CC'CwG_GG

Cuts at:      0     986    1011 
   Size:        986      25
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      61      93    1011 
   Size:         61      32     918

  Fragments arranged by size: 

                 918      61      32
 
SacI G_AGCT'C

Cuts at:      0     415    1011 
   Size:        415     596
 
SacII CC_GC'GG

Cuts at:      0     818     896    1011 
   Size:        818      78     115

  Fragments arranged by size: 

                 818     115      78
 
ScaI AGT'ACT

Cuts at:      0      90    1011 
   Size:         90     921
 
SspI AAT'ATT

Cuts at:      0     275    1011 
   Size:        275     736
 
StuI AGG'CCT

Cuts at:      0     425    1011 
   Size:        425     586
 
StyI C'CwwG_G

Cuts at:      0     353     515    1011 
   Size:        353     162     496

  Fragments arranged by size: 

                 496     353     162
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     499    1011 
   Size:        499     512
 
TatI w'GTAC_w

Cuts at:      0      88    1011 
   Size:         88     923
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     815     847     924     956    1011 
   Size:        815      32      77      32      55

  Fragments arranged by size: 

                 815      77      55      32      32
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     ApoI     AvaI    BamHI     BanI    BanII
     BbsI    BbvCI     BglI    BmgBI     BmrI     BplI     BpmI   Bpu10I
     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BstXI    BstYI     BtgI
     BtsI     EaeI  EcoICRI  Eco57MI    EcoRI    HaeII    Hin4I   HincII
     KpnI     MmeI     MslI   MspA1I     NcoI     PasI     PpiI     SacI
    SacII     ScaI     SspI     StuI     StyI    TaqII     TatI     TstI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI     BbeI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI Bme1580I     BmtI    BpuEI    BsaAI    BsaBI    BsaWI
    BsiEI    BsiWI    BspEI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI
     MfeI     MluI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI     PfoI
     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SalI    SanDI     SapI     SbfI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SwaI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI
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