Restriction map for pF1KB7327
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       AclI        AcyI        AflII       AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       AscI        AspEI       AspHI       AvaI        
AviII       AvrII       BalI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BfrI        
BglI        BlnI        BlpI        BmyI        Bpu1102I    
BsaAI       BsaHI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsoBI       Bsp1286I    Bsp1407I    BspHI       
BspMI       BsrFI       BsrGI       BssHII      Bst1107I    
BstEII      BstPI       BstXI       BstZ17I     CelII       
CfoI        Cfr10I      CpoI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoT14I     EcoT22I     FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HpaI        HphI        KasI        MluI        MluNI       
MscI        NaeI        NarI        NcoI        NgoMIV      
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PflMI       PinAI       PmaCI       PmeI        
PmlI        Psp1406I    PvuI        RcaI        RsrII       
SacI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SmlI        
SnaBI       SphI        Sse8387I    SspBI       StuI        
StyI        SwaI        Van91I      XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccII          18  CGCG                 CG^CG
AccIII        605  TCCGGA               T^CCGGA
AflIII       1469  ACATGT               A^C[GA][CT]GT
AocI         2302  CCTAAGG              CC^T[ACGT]AGG
Asp700I       511  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          598  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbsI         1349  GAAGAC               GAAGAC(2/6)
BcnI           81  CCCGG                CC^[GC]GG
BpuAI        1349  GAAGAC               GAAGAC(2/6)
BsaBI        2380  GATAAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1013  GAGACC               GGTCTC(1/5)
BsaWI         605  TCCGGA               [AT]^CCGG[AT]
BseAI         605  TCCGGA               T^CCGGA
BsgI         1318  CTGCAC               GTGCAG(16/14)
BsiYI        2091  CCCCCAGTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI         2091  CCCCCAGTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI        1013  GAGAC                GTCTC(1/5)
BsmFI         961  GGGAC                GGGAC(10/14)
BspEI         605  TCCGGA               T^CCGGA
BspLU11I     1469  ACATGT               A^CATGT
BsrBI         133  GAGCGG               CCGCTC(-3/-3)
BssSI        2021  CACGAG               CACGAG(-5/-1)
BstAPI       1311  GCATATGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         534  TTCGAA               TT^CGAA
BstUI          18  CGCG                 CG^CG
Bsu36I       2302  CCTAAGG              CC^T[ACGT]AGG
DraI          318  TTTAAA               TTT^AAA
DraII         813  AGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII       1846  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI           17  CCGCGG               C^C[GA][CT]GG
EaeI           83  CGGCCA               [CT]^GGCC[GA]
Eco81I       2302  CCTAAGG              CC^T[ACGT]AGG
EcoO109I      813  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI         865  GAATTC               G^AATTC
EcoRV         886  GATATC               GAT^ATC
HindIII      1227  AAGCTT               A^AGCTT
KspI           17  CCGCGG               CCGC^GG
MamI         2380  GATAAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          605  TCCGGA               T^CCGGA
MvnI           18  CGCG                 CG^CG
NciI           81  CCCGG                CC^[GC]GG
NdeI         1312  CATATG               CA^TATG
NspV          534  TTCGAA               TT^CGAA
PflFI         598  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         813  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI        1352  GACTGAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII        2207  CAGCTG               CAG^CTG
SacII          17  CCGCGG               CCGC^GG
SfuI          534  TTCGAA               TT^CGAA
SpeI         1252  ACTAGT               A^CTAGT
Tsp45I       1844  GTCAC                ^GT[GC]AC
Tth111I       598  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI          511  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

Acc65I        191  GGTACC               G^GTACC
              674  GGTACC              
Asp718I       191  GGTACC               G^GTACC
              674  GGTACC              
BamHI        1477  GGATCC               G^GATCC
             1989  GGATCC              
BanI          191  GGTACC               G^G[CT][GA]CC
              674  GGTACC              
BpmI           41  CTCCAG               CTGGAG(16/14)
              656  CTGGAG              
BsmI         1817  GAATGC               GAATGC(1/-1)
             2430  GAATGC              
BspDI        1768  ATCGAT               AT^CGAT
             2182  ATCGAT              
ClaI         1768  ATCGAT               AT^CGAT
             2182  ATCGAT              
FauI           16  CCCGC                CCCGC(4/6)
               19  GCGGG               
HapII          82  CCGG                 C^CGG
              606  CCGG                
HincII        177  GTCGAC               GT[CT]^[GA]AC
             1517  GTCGAC              
HindII        177  GTCGAC               GT[CT]^[GA]AC
             1517  GTCGAC              
HpaII          82  CCGG                 C^CGG
              606  CCGG                
KpnI          191  GGTACC               GGTAC^C
              674  GGTACC              
MaeII         924  ACGT                 A^CGT
              991  ACGT                
MslI           66  CACTGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1887  CATTTTGATG          
MspI           82  CCGG                 C^CGG
              606  CCGG                
PstI         1678  CTGCAG               CTGCA^G
             2051  CTGCAG              
SalI          177  GTCGAC               G^TCGAC
             1517  GTCGAC              
SspI          409  AATATT               AAT^ATT
              943  AATATT              
XbaI         2329  TCTAGA               T^CTAGA
             2388  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AseI        AsnI        AvaII       
BbvI        BclI        BfaI        BglII       BsaJI       
BseRI       BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       EarI        EcoRII      FbaI        Fnu4HI      
FokI        HaeIII      HinfI       ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MfeI        
MflI        MnlI        MseI        MspA1I      MunI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PleI        PshBI       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TaqI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     XhoII       
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