Restriction map for pF1KE0438
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# NO CUT ENZYMES (  115 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AflIII      
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BbsI        BbvCI       BcgI        
BclI        BfrI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaBI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BsmI        
BspDI       BspEI       BspLU11I    BsrBI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     CelII       ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        Ksp632I     
KspI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PmeI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        Tth111I     XbaI        XhoI        


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# ONE CUT ENZYMES (   65 enzymes) #
###################################

AatII         739  GACGTC               GACGT^C
AccI          600  GTCTAC               GT^[AC][GT]AC
AccII          63  CGCG                 CG^CG
AcsI          580  GAATTC               [GA]^AATT[CT]
AgeI            6  ACCGGT               A^CCGGT
AlwNI         791  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          565  CCTGAGG              CC^T[ACGT]AGG
ApaI          152  GGGCCC               GGGCC^C
ApoI          580  GAATTC               [GA]^AATT[CT]
AspHI         459  GAGCAC               G[AT]GC[AT]^C
AvaI           71  CCCGGG               C^[CT]CG[GA]G
AvaII         413  GGACC                G^G[AT]CC
BalI          773  TGGCCA               TGG^CCA
BanI          551  GGCGCC               G^G[CT][GA]CC
BbeI          551  GGCGCC               GGCGC^C
BbrPI         184  CACGTG               CAC^GTG
BciVI          41  GGATAC               GTATCC(6/5)
BsaAI         184  CACGTG               [CT]AC^GT[GA]
BsaI            3  GAGACC               GGTCTC(1/5)
BsaWI           6  ACCGGT               [AT]^CCGG[AT]
BseRI         172  CTCCTC               GAGGAG(10/8)
BsiHKAI       459  GAGCAC               G[AT]GC[AT]^C
BsoBI          71  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      542  TGTACA               T^GTACA
BspHI          17  TCATGA               T^CATGA
BsrDI         501  CATTGC               GCAATG(2/0)
BsrGI         542  TGTACA               T^GTACA
BssSI         417  CTCGTG               CACGAG(-5/-1)
BstUI          63  CGCG                 CG^CG
BstXI         239  CCATGTCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        565  CCTGAGG              CC^T[ACGT]AGG
DraII         151  GGGGCCC              [GA]G^G[ACGT]CC[CT]
DsaI          156  CCACGG               C^C[GA][CT]GG
EaeI          773  TGGCCA               [CT]^GGCC[GA]
Eco81I        565  CCTGAGG              CC^T[ACGT]AGG
EcoNI         759  CCTGTACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      151  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         580  GAATTC               G^AATTC
HaeII         551  GGCGCC               [GA]GCGC^[CT]
HgaI          510  GCGTC                GACGC(5/10)
HphI           32  TCACC                GGTGA(8/7)
KasI          551  GGCGCC               G^GCGCC
MluNI         773  TGGCCA               TGG^CCA
MscI          773  TGGCCA               TGG^CCA
MseI          652  TTAA                 T^TAA
MvnI           63  CGCG                 CG^CG
NarI          551  GGCGCC               GG^CGCC
PflMI         765  CCAGGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI           6  ACCGGT               A^CCGGT
PmaCI         184  CACGTG               CAC^GTG
PmlI          184  CACGTG               CAC^GTG
PstI          209  CTGCAG               CTGCA^G
RcaI           17  TCATGA               T^CATGA
SfcI          209  CTGCAG               C^T[GA][CT]AG
SfoI          551  GGCGCC               GGC^GCC
SmaI           71  CCCGGG               CCC^GGG
SmlI          976  CTCAAG               C^T[CT][GA]AG
SspBI         542  TGTACA               T^GTACA
TfiI          867  GATTC                G^A[AT]TC
Tru9I         652  TTAA                 T^TAA
Tsp509I       581  AATT                 ^AATT
Van91I        765  CCAGGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          911  CCATGTCCTTGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI          71  CCCGGG               C^CCGGG
XmaI           71  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AfaI          543  GTAC                 GT^AC
              762  GTAC                
Asp700I        21  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              516  GAAACCATTC          
BamHI         197  GGATCC               G^GATCC
              290  GGATCC              
BcnI           71  CCCGG                CC^[GC]GG
               72  CCGGG               
BfaI          133  CTAG                 C^TAG
              968  CTAG                
BglI           70  GCCCGGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              426  GCCCCTCTGGC         
BsmAI           3  GAGAC                GTCTC(1/5)
              346  GTCTC               
BsmFI         101  GGGAC                GGGAC(10/14)
              669  GGGAC               
BspMI         364  ACCTGC               ACCTGC(4/8)
              657  ACCTGC              
BsrFI           6  ACCGGT               [GA]^CCGG[CT]
              371  GCCGGT              
BsrI          801  ACTGG                ACTGG(1/-1)
              776  CCAGT               
BstYI         197  GGATCC               [GA]^GATC[CT]
              290  GGATCC              
Cfr10I          6  ACCGGT               [GA]^CCGG[CT]
              371  GCCGGT              
DpnII         198  GATC                 ^GATC
              291  GATC                
EcoT14I       125  CCAAGG               C^C[AT][AT]GG
              770  CCTTGG              
MaeI          133  CTAG                 C^TAG
              968  CTAG                
MaeII         185  ACGT                 A^CGT
              740  ACGT                
MaeIII         31  GTCAC                ^GT[ACGT]AC
              808  GTGAC               
MboI          198  GATC                 ^GATC
              291  GATC                
MflI          197  GGATCC               [GA]^GATC[CT]
              290  GGATCC              
NciI           71  CCCGG                CC^[GC]GG
               72  CCGGG               
NdeII         198  GATC                 ^GATC
              291  GATC                
PvuII         710  CAGCTG               CAG^CTG
              791  CAGCTG              
RsaI          543  GTAC                 GT^AC
              762  GTAC                
Sau3AI        198  GATC                 ^GATC
              291  GATC                
StuI          562  AGGCCT               AGG^CCT
              767  AGGCCT              
StyI          125  CCAAGG               C^C[AT][AT]GG
              770  CCTTGG              
TaqI          254  TCGA                 T^CGA
              389  TCGA                
Tsp45I         31  GTCAC                ^GT[GC]AC
              808  GTGAC               
TthHB8I       254  TCGA                 T^CGA
              389  TCGA                
XhoII         197  GGATCC               [GA]^GATC[CT]
              290  GGATCC              
XmnI           21  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              516  GAAACCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AciI        AcyI        AluI        AlwI        BanII       
BbvI        BmyI        BsaHI       BsaJI       BsiYI       
BslI        Bsp1286I    BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        Hin1I       HinP1I      HinfI       HpaII       
ItaI        MboII       MnlI        MslI        MspA1I      
MspI        MvaI        MwoI        NlaIII      NlaIV       
PleI        Sau96I      ScrFI       SfaNI       TseI        
TspRI       
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