Restriction map for pF1KE0438
 (Linear) (Six-base) MAPSORT of: KIEE0438.seq  Check: 8997  from: 1  to: 984



KIEE0438.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     373     666     984 
   Size:        373     293     318

  Fragments arranged by size: 

                 373     318     293
 
AatII G_ACGT'C

Cuts at:      0     743     984 
   Size:        743     241
 
AccI GT'mk_AC

Cuts at:      0     601     984 
   Size:        601     383
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     874     984 
   Size:        874     110
 
AgeI A'CCGG_T

Cuts at:      0       6     984 
   Size:          6     978
 
AlwNI CAG_nnn'CTG

Cuts at:      0     796     984 
   Size:        796     188
 
ApaI G_GGCC'C

Cuts at:      0     156     984 
   Size:        156     828
 
ApoI r'AATT_y

Cuts at:      0     580     984 
   Size:        580     404
 
AvaI C'yCGr_G

Cuts at:      0      71     984 
   Size:         71     913
 
BamHI G'GATC_C

Cuts at:      0     197     290     984 
   Size:        197      93     694

  Fragments arranged by size: 

                 694     197      93
 
BanI G'GyrC_C

Cuts at:      0     551     984 
   Size:        551     433
 
BanII G_rGCy'C

Cuts at:      0     116     156     817     984 
   Size:        116      40     661     167

  Fragments arranged by size: 

                 661     167     116      40
 
BbeI G_GCGC'C

Cuts at:      0     555     984 
   Size:        555     429
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      35     984 
   Size:         35     949
 
BglI GCCn_nnn'nGGC

Cuts at:      0      76     432     984 
   Size:         76     356     552

  Fragments arranged by size: 

                 552     356      76
 
Bme1580I G_kGCm'C

Cuts at:      0     156     984 
   Size:        156     828
 
Bpu10I CC'TnA_GC

Cuts at:      0     608     984 
   Size:        608     376
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     961     984 
   Size:        961      23
 
BsaAI yAC'GTr

Cuts at:      0     186     984 
   Size:        186     798
 
BsaHI Gr'CG_yC

Cuts at:      0     510     552     740     984 
   Size:        510      42     188     244

  Fragments arranged by size: 

                 510     244     188      42
 
BsaWI w'CCGG_w

Cuts at:      0       6     984 
   Size:          6     978
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     163     984 
   Size:        163     821
 
BseYI C'CCAG_C

Cuts at:      0     480     984 
   Size:        480     504
 
BsiHKAI G_wGCw'C

Cuts at:      0     463     984 
   Size:        463     521
 
Bsp1286I G_dGCh'C

Cuts at:      0     116     156     463     817     984 
   Size:        116      40     307     354     167

  Fragments arranged by size: 

                 354     307     167     116      40
 
BspHI T'CATG_A

Cuts at:      0      17     984 
   Size:         17     967
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     373     666     984 
   Size:        373     293     318

  Fragments arranged by size: 

                 373     318     293
 
BsrDI GCAATG_nn'

Cuts at:      0     500     984 
   Size:        500     484
 
BsrFI r'CCGG_y

Cuts at:      0       6     371     984 
   Size:          6     365     613

  Fragments arranged by size: 

                 613     365       6
 
BsrGI T'GTAC_A

Cuts at:      0     542     984 
   Size:        542     442
 
BssSI C'ACGA_G

Cuts at:      0     417     984 
   Size:        417     567
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     246     984 
   Size:        246     738
 
BstYI r'GATC_y

Cuts at:      0     197     290     984 
   Size:        197      93     694

  Fragments arranged by size: 

                 694     197      93
 
Bsu36I CC'TnA_GG

Cuts at:      0     566     984 
   Size:        566     418
 
BtgI C'CryG_G

Cuts at:      0     156     984 
   Size:        156     828
 
BtsI GCAGTG_nn'

Cuts at:      0     103     823     891     984 
   Size:        103     720      68      93

  Fragments arranged by size: 

                 720     103      93      68
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     267     302     984 
   Size:        267      35     682

  Fragments arranged by size: 

                 682     267      35
 
EaeI y'GGCC_r

Cuts at:      0     773     984 
   Size:        773     211
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     874     984 
   Size:        874     110
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     763     984 
   Size:        763     221
 
EcoO109I rG'GnC_Cy

Cuts at:      0     152     984 
   Size:        152     832
 
EcoRI G'AATT_C

Cuts at:      0     580     984 
   Size:        580     404
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     705     984 
   Size:        705     279
 
HaeII r_GCGC'y

Cuts at:      0     555     984 
   Size:        555     429
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     583     615     984 
   Size:        583      32     369

  Fragments arranged by size: 

                 583     369      32
 
KasI G'GCGC_C

Cuts at:      0     551     984 
   Size:        551     433
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     984 
   Size:        203     781
 
MscI TGG'CCA

Cuts at:      0     775     984 
   Size:        775     209
 
MslI CAynn'nnrTG

Cuts at:      0      22     244     637     984 
   Size:         22     222     393     347

  Fragments arranged by size: 

                 393     347     222      22
 
MspA1I CmG'CkG

Cuts at:      0     577     712     793     984 
   Size:        577     135      81     191

  Fragments arranged by size: 

                 577     191     135      81
 
NarI GG'CG_CC

Cuts at:      0     552     984 
   Size:        552     432
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     771     984 
   Size:        771     213
 
PfoI T'CCnGG_A

Cuts at:      0     200     984 
   Size:        200     784
 
PmlI CAC'GTG

Cuts at:      0     186     984 
   Size:        186     798
 
PspOMI G'GGCC_C

Cuts at:      0     152     984 
   Size:        152     832
 
PstI C_TGCA'G

Cuts at:      0     213     984 
   Size:        213     771
 
PvuII CAG'CTG

Cuts at:      0     712     793     984 
   Size:        712      81     191

  Fragments arranged by size: 

                 712     191      81
 
SfcI C'TryA_G

Cuts at:      0     209     984 
   Size:        209     775
 
SfoI GGC'GCC

Cuts at:      0     553     984 
   Size:        553     431
 
SmaI CCC'GGG

Cuts at:      0      73     984 
   Size:         73     911
 
SmlI C'TyrA_G

Cuts at:      0     976     984 
   Size:        976       8
 
SrfI GCCC'GGGC

Cuts at:      0      73     984 
   Size:         73     911
 
StuI AGG'CCT

Cuts at:      0     564     769     984 
   Size:        564     205     215

  Fragments arranged by size: 

                 564     215     205
 
StyI C'CwwG_G

Cuts at:      0     125     770     984 
   Size:        125     645     214

  Fragments arranged by size: 

                 645     214     125
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     907     984 
   Size:        907      77
 
TatI w'GTAC_w

Cuts at:      0     542     984 
   Size:        542     442
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     791     823     984 
   Size:        791      32     161

  Fragments arranged by size: 

                 791     161      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     918     984 
   Size:        918      66
 
XmaI C'CCGG_G

Cuts at:      0      71     984 
   Size:         71     913
 
XmnI GAAnn'nnTTC

Cuts at:      0      25     520     984 
   Size:         25     495     464

  Fragments arranged by size: 

                 495     464      25
 
ZraI GAC'GTC

Cuts at:      0     741     984 
   Size:        741     243
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AgeI    AlwNI     ApaI     ApoI
     AvaI    BamHI     BanI    BanII     BbeI    BciVI     BglI Bme1580I
   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BseRI    BseYI  BsiHKAI
 Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BsrGI    BssSI    BstXI
    BstYI   Bsu36I     BtgI     BtsI    CspCI     EaeI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI    HaeII    Hin4I     KasI     MmeI     MscI
     MslI   MspA1I     NarI    PflMI     PfoI     PmlI   PspOMI     PstI
    PvuII     SfcI     SfoI     SmaI     SmlI     SrfI     StuI     StyI
    TaqII     TatI     TstI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII   AflIII     AhdI     AleI     AlfI
     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbsI
    BbvCI     BcgI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI     BsaI    BsaBI    BsaXI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BsrBI   BssHII   BstAPI    BstBI
   BstEII  BstZ17I    BtgZI     ClaI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoRV     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     NaeI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SspI     SwaI  Tth111I     XbaI     XhoI
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