################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspHI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BlnI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI Ksp632I KspI MaeII MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 80 GGTACC G^GTACC AcsI 898 AAATTC [GA]^AATT[CT] AflIII 764 ACATGT A^C[GA][CT]GT AlwI 413 GGATC GGATC(4/5) AlwNI 797 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 898 AAATTC [GA]^AATT[CT] Asp700I 699 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 80 GGTACC G^GTACC AvaII 641 GGACC G^G[AT]CC BalI 224 TGGCCA TGG^CCA BfaI 149 CTAG C^TAG BglII 659 AGATCT A^GATCT BlpI 345 GCTGAGC GC^T[ACGT]AGC BmyI 574 GTGCCC G[AGT]GC[ACT]^C BpmI 733 CTGGAG CTGGAG(16/14) Bpu1102I 345 GCTGAGC GC^T[ACGT]AGC BseRI 200 GAGGAG GAGGAG(10/8) BsiYI 130 CCAACCCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 130 CCAACCCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 574 GTGCCC G[AGT]GC[ACT]^C BspLU11I 764 ACATGT A^CATGT BspMI 77 GCAGGT ACCTGC(4/8) BsrDI 816 CATTGC GCAATG(2/0) BstXI 644 CCACGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 659 AGATCT [GA]^GATC[CT] CelII 345 GCTGAGC GC^T[ACGT]AGC Cfr13I 641 GGACC G^G[ACGT]CC DraI 475 TTTAAA TTT^AAA EcoRV 670 GATATC GAT^ATC EcoT14I 694 CCTTGG C^C[AT][AT]GG EcoT22I 498 ATGCAT ATGCA^T HgaI 391 GCGTC GACGC(5/10) HincII 603 GTCAAC GT[CT]^[GA]AC HindII 603 GTCAAC GT[CT]^[GA]AC KpnI 80 GGTACC GGTAC^C MaeI 149 CTAG C^TAG MflI 659 AGATCT [GA]^GATC[CT] MluNI 224 TGGCCA TGG^CCA MscI 224 TGGCCA TGG^CCA MspA1I 569 CAGCGG C[AC]G^C[GT]G NdeI 597 CATATG CA^TATG NheI 148 GCTAGC G^CTAGC NsiI 498 ATGCAT ATGCA^T PstI 8 CTGCAG CTGCA^G Sau96I 641 GGACC G^G[ACGT]CC SfcI 8 CTGCAG C^T[GA][CT]AG StyI 694 CCTTGG C^C[AT][AT]GG TfiI 300 GATTC G^A[AT]TC XhoII 659 AGATCT [GA]^GATC[CT] XmnI 699 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AluI 60 AGCT AG^CT 801 AGCT BcnI 245 CCGGG CC^[GC]GG 709 CCCGG BsmI 178 GAATGC GAATGC(1/-1) 699 GAATGC Cac8I 148 GCTAGC GC[ACGT]^[ACGT]GC 286 GCAGGC DdeI 346 CTGAG C^T[ACGT]AG 567 CTCAG DsaI 293 CCGTGG C^C[GA][CT]GG 644 CCACGG EaeI 224 TGGCCA [CT]^GGCC[GA] 492 TGGCCG HapII 245 CCGG C^CGG 710 CCGG HpaII 245 CCGG C^CGG 710 CCGG MaeIII 332 GTGAC ^GT[ACGT]AC 624 GTCAC MboII 883 GAAGA GAAGA(8/7) 401 TCTTC MseI 476 TTAA T^TAA 872 TTAA MslI 525 CACCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 645 CACGGGTGTG MspI 245 CCGG C^CGG 710 CCGG NciI 245 CCGGG CC^[GC]GG 709 CCCGG NspI 273 GCATGT [GA]CATG^[CT] 764 ACATGT PleI 203 GAGTC GAGTC(4/5) 876 GAGTC SmlI 156 CTTGAG C^T[CT][GA]AG 304 CTTGAG Tru9I 476 TTAA T^TAA 872 TTAA Tsp45I 332 GTGAC ^GT[GC]AC 624 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI AfaI BanI BbvI BsaJI BsgI BsmFI BsrI BssKI BstF5I BstNI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MboI MnlI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI SfaNI TseI Tsp509I TspRI