################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvrII BamHI BanI BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglII BlnI BsaAI BsaHI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FauI FbaI FseI HaeII Hin1I HindIII KasI KpnI KspI MflI MluI MroI MvnI NarI NciI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 649 GTCTAC GT^[AC][GT]AC AlwI 342 GATCC GGATC(4/5) AlwNI 178 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 415 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 47 GTGCTC G[AT]GC[AT]^C AviII 890 TGCGCA TGC^GCA BalI 1523 TGGCCA TGG^CCA BbsI 762 GAAGAC GAAGAC(2/6) BbvCI 582 CCTCAGC CCTCAGC(-5/-2) BglI 1525 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 175 GCTCAGC GC^T[ACGT]AGC BpmI 619 CTCCAG CTGGAG(16/14) Bpu1102I 175 GCTCAGC GC^T[ACGT]AGC BpuAI 762 GAAGAC GAAGAC(2/6) BsaBI 1044 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 1049 CGATCG CG[GA][CT]^CG BsiHKAI 47 GTGCTC G[AT]GC[AT]^C BsrBI 795 GAGCGG CCGCTC(-3/-3) BsrFI 2096 GCCGGC [GA]^CCGG[CT] BstXI 731 CCAATTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 175 GCTCAGC GC^T[ACGT]AGC Cfr10I 2096 GCCGGC [GA]^CCGG[CT] DraI 1368 TTTAAA TTT^AAA DraII 870 GGGACCT [GA]G^G[ACGT]CC[CT] EaeI 1523 TGGCCA [CT]^GGCC[GA] EcoO109I 870 GGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 345 CCATGG C^C[AT][AT]GG FspI 890 TGCGCA TGC^GCA HapII 2097 CCGG C^CGG HgaI 910 GACGC GACGC(5/10) HpaII 2097 CCGG C^CGG MamI 1044 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1523 TGGCCA TGG^CCA MscI 1523 TGGCCA TGG^CCA MspA1I 2056 CAGCTG C[AC]G^C[GT]G MspI 2097 CCGG C^CGG NaeI 2096 GCCGGC GCC^GGC NcoI 345 CCATGG C^CATGG NgoMIV 2096 GCCGGC G^CCGGC NspI 789 GCATGT [GA]CATG^[CT] PflMI 931 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 883 GACTC GAGTC(4/5) PpuMI 870 GGGACCT [GA]G^G[AT]CC[CT] PvuI 1049 CGATCG CGAT^CG PvuII 2056 CAGCTG CAG^CTG SmlI 757 CTCAAG C^T[CT][GA]AG StuI 1511 AGGCCT AGG^CCT StyI 345 CCATGG C^C[AT][AT]GG Van91I 931 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 415 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AciI 1493 CCGC CCGC(-3/-1) 797 GCGG AfaI 1570 GTAC GT^AC 1664 GTAC BanII 596 GAGCCC G[GA]GC[CT]^C 1425 GAGCCC BfaI 256 CTAG C^TAG 442 CTAG BsgI 1635 CTGCAC GTGCAG(16/14) 1450 GTGCAG BspHI 377 TCATGA T^CATGA 423 TCATGA BspMI 437 ACCTGC ACCTGC(4/8) 1297 GCAGGT BsrDI 470 CATTGC GCAATG(2/0) 693 CATTGC DsaI 345 CCATGG C^C[GA][CT]GG 866 CCACGG HincII 148 GTTAAC GT[CT]^[GA]AC 1833 GTTAAC HindII 148 GTTAAC GT[CT]^[GA]AC 1833 GTTAAC HpaI 148 GTTAAC GTT^AAC 1833 GTTAAC MaeI 256 CTAG C^TAG 442 CTAG MfeI 558 CAATTG C^AATTG 644 CAATTG MunI 558 CAATTG C^AATTG 644 CAATTG RcaI 377 TCATGA T^CATGA 423 TCATGA RsaI 1570 GTAC GT^AC 1664 GTAC TaqI 1821 TCGA T^CGA 2135 TCGA Tsp45I 265 GTCAC ^GT[GC]AC 1540 GTGAC TthHB8I 1821 TCGA T^CGA 2135 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AcsI AluI ApoI AvaII BbvI BmyI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII EcoRV Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PstI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp509I TspRI XcmI