Restriction map for pF1KB6468
##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BamHI       BanI        BanII       BbeI        BbrPI       
BciVI       BclI        BfrI        BglI        BlnI        
BlpI        BmyI        Bpu1102I    BsaAI       BsaHI       
BsaI        BsaWI       BseAI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoRV       
FauI        FbaI        FseI        FspI        HgaI        
Hin1I       KasI        KpnI        KspI        MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI          217  GTCTAC               GT^[AC][GT]AC
AfaI          406  GTAC                 GT^AC
AlwI          425  GATCC                GGATC(4/5)
BalI          606  TGGCCA               TGG^CCA
BbvCI         703  GCTGAGG              CCTCAGC(-5/-2)
BcnI          311  CCCGG                CC^[GC]GG
BfaI          367  CTAG                 C^TAG
BsaBI         156  GATGAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI          57  CGACCG               CG[GA][CT]^CG
BsmFI         264  GGGAC                GGGAC(10/14)
BsmI          725  GAATGC               GAATGC(1/-1)
BsrDI         243  CATTGC               GCAATG(2/0)
BstAPI        301  GCAGATGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI           17  GCGC                 GCG^C
Cfr13I        675  GGCCC                G^G[ACGT]CC
DsaI          260  CCATGG               C^C[GA][CT]GG
EcoNI         816  CCTTTTTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       727  ATGCAT               ATGCA^T
HaeII          16  AGCGCC               [GA]GCGC^[CT]
HapII         312  CCGG                 C^CGG
HhaI           17  GCGC                 GCG^C
HinP1I         17  GCGC                 G^CGC
HincII        846  GTTAAC               GT[CT]^[GA]AC
HindII        846  GTTAAC               GT[CT]^[GA]AC
HindIII       410  AAGCTT               A^AGCTT
HpaI          846  GTTAAC               GTT^AAC
HpaII         312  CCGG                 C^CGG
MaeI          367  CTAG                 C^TAG
MaeII          10  ACGT                 A^CGT
MamI          156  GATGAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         606  TGGCCA               TGG^CCA
MscI          606  TGGCCA               TGG^CCA
MspA1I        896  CAGCTG               C[AC]G^C[GT]G
MspI          312  CCGG                 C^CGG
NciI          311  CCCGG                CC^[GC]GG
NcoI          260  CCATGG               C^CATGG
NdeI          884  CATATG               CA^TATG
NlaIV         874  GGTTCC               GG[ACGT]^[ACGT]CC
NsiI          727  ATGCAT               ATGCA^T
PvuII         896  CAGCTG               CAG^CTG
RsaI          406  GTAC                 GT^AC
Sau96I        675  GGCCC                G^G[ACGT]CC
SfcI          219  CTACAG               C^T[GA][CT]AG
TaqI           56  TCGA                 T^CGA
Tsp45I        652  GTGAC                ^GT[GC]AC
TthHB8I        56  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

BbsI           22  GAAGAC               GAAGAC(2/6)
              537  GAAGAC              
BcgI          986  CGAAGAATGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
               47  GCAAGATGATCG        
BglII         924  AGATCT               A^GATCT
              953  AGATCT              
BpmI          359  CTCCAG               CTGGAG(16/14)
              122  CTGGAG              
BpuAI          22  GAAGAC               GAAGAC(2/6)
              537  GAAGAC              
BseRI         712  GAGGAG               GAGGAG(10/8)
              837  GAGGAG              
BsgI            6  CTGCAC               GTGCAG(16/14)
              994  GTGCAG              
BspHI         168  TCATGA               T^CATGA
              353  TCATGA              
BsrI          489  CCAGT                ACTGG(1/-1)
              915  CCAGT               
BstF5I        174  GGATG                GGATG(2/0)
              189  GGATG               
BstNI         296  CCAGG                CC^[AT]GG
              428  CCAGG               
Cac8I         331  GCTGGC               GC[ACGT]^[ACGT]GC
              412  GCTTGC              
EaeI           89  CGGCCA               [CT]^GGCC[GA]
              606  TGGCCA              
EarI          796  CTCTTC               CTCTTC(1/4)
              889  GAAGAG              
EcoRII        296  CCAGG                ^CC[AT]GG
              428  CCAGG               
EcoT14I       260  CCATGG               C^C[AT][AT]GG
              947  CCAAGG              
FokI          174  GGATG                GGATG(9/13)
              189  GGATG               
HphI          566  TCACC                GGTGA(8/7)
              968  TCACC               
Ksp632I       796  CTCTTC               CTCTTC(1/4)
              889  GAAGAG              
MaeIII        652  GTGAC                ^GT[ACGT]AC
             1015  GTTAC               
MslI          141  CACCATTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              169  CATGAGGATG          
MvaI          296  CCAGG                CC^[AT]GG
              428  CCAGG               
RcaI          168  TCATGA               T^CATGA
              353  TCATGA              
SfaNI         307  GATGC                GCATC(5/9)
             1009  GATGC               
StyI          260  CCATGG               C^C[AT][AT]GG
              947  CCAAGG              
Tsp509I       317  AATT                 ^AATT
              660  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AciI        AluI        BbvI        BsaJI       BsiYI       
BslI        BssKI       BstYI       DdeI        DpnII       
Fnu4HI      HaeIII      HinfI       ItaI        MboI        
MboII       MflI        MnlI        MseI        MwoI        
NdeII       NlaIII      PleI        Sau3AI      ScrFI       
TfiI        Tru9I       TseI        TspRI       XhoII       
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