Restriction map for pF1KB6563
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AgeI        
AhdI        Alw44I      AocI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BbrPI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmAI       BsmBI       
BsmI        BspDI       BspEI       BsrDI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MunI        NdeI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacI        
SalI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        Sse8387I    
SspI        StuI        SwaI        Tru9I       Tth111I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AatII         622  GACGTC               GACGT^C
AccI          244  GTCTAC               GT^[AC][GT]AC
AccII         118  CGCG                 CG^CG
AcsI          457  GAATTC               [GA]^AATT[CT]
Aor51HI       386  AGCGCT               AGC^GCT
ApoI          457  GAATTC               [GA]^AATT[CT]
Asp700I       252  GAACATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI          199  GGCGCC               GGCGC^C
BbsI         1063  GTCTTC               GAAGAC(2/6)
BbvCI         913  GCTGAGG              CCTCAGC(-5/-2)
BfaI          338  CTAG                 C^TAG
BglI          977  GCCCCGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI        1063  GTCTTC               GAAGAC(2/6)
BsaBI         183  GATGCGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          595  GTGCAG               GTGCAG(16/14)
BspHI         347  TCATGA               T^CATGA
BssSI         948  CACGAG               CACGAG(-5/-1)
BstUI         118  CGCG                 CG^CG
EaeI          398  CGGCCA               [CT]^GGCC[GA]
Eco47III      386  AGCGCT               AGC^GCT
EcoNI          39  CCTGACGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         457  GAATTC               G^AATTC
EcoT14I       287  CCATGG               C^C[AT][AT]GG
HphI          509  TCACC                GGTGA(8/7)
KasI          199  GGCGCC               G^GCGCC
KspI          117  CCGCGG               CCGC^GG
MaeI          338  CTAG                 C^TAG
MaeIII        370  GTGAC                ^GT[ACGT]AC
MamI          183  GATGCGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I        117  CCGCGG               C[AC]G^C[GT]G
MvnI          118  CGCG                 CG^CG
NarI          199  GGCGCC               GG^CGCC
NcoI          287  CCATGG               C^CATGG
NheI          337  GCTAGC               G^CTAGC
PleI         1081  GACTC                GAGTC(4/5)
PpuMI         356  AGGACCC              [GA]G^G[AT]CC[CT]
PstI          298  CTGCAG               CTGCA^G
RcaI          347  TCATGA               T^CATGA
SacII         117  CCGCGG               CCGC^GG
SapI          162  GAAGAGC              GCTCTTC(1/4)
ScaI          581  AGTACT               AGT^ACT
SfcI          298  CTGCAG               C^T[GA][CT]AG
SfoI          199  GGCGCC               GGC^GCC
SmaI           65  CCCGGG               CCC^GGG
SphI          557  GCATGC               GCATG^C
StyI          287  CCATGG               C^C[AT][AT]GG
Tsp45I        370  GTGAC                ^GT[GC]AC
Tsp509I       458  AATT                 ^AATT
XcmI          860  CCACCTCCCACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI          65  CCCGGG               C^CCGGG
XmaI           65  CCCGGG               C^CCGGG
XmnI          252  GAACATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AflIII        941  ACATGT               A^C[GA][CT]GT
             1030  ACATGT              
AlwNI         406  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              718  CAGTGCCTG           
ApaI          144  GGGCCC               GGGCC^C
              897  GGGCCC              
BamHI          89  GGATCC               G^GATCC
              656  GGATCC              
BanI           35  GGTGCC               G^G[CT][GA]CC
              199  GGCGCC              
BanII         144  GGGCCC               G[GA]GC[CT]^C
              897  GGGCCC              
Bsp1407I      944  TGTACA               T^GTACA
              956  TGTACA              
BspLU11I      941  ACATGT               A^CATGT
             1030  ACATGT              
BsrFI         202  GCCGGC               [GA]^CCGG[CT]
              424  GCCGGC              
BsrGI         944  TGTACA               T^GTACA
              956  TGTACA              
BstYI          89  GGATCC               [GA]^GATC[CT]
              656  GGATCC              
Cfr10I        202  GCCGGC               [GA]^CCGG[CT]
              424  GCCGGC              
DraIII        701  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
              960  CACCGGGTG           
EarI         1015  CTCTTC               CTCTTC(1/4)
              162  GAAGAG              
HgaI         1010  GACGC                GACGC(5/10)
              447  GCGTC               
Ksp632I      1015  CTCTTC               CTCTTC(1/4)
              162  GAAGAG              
MflI           89  GGATCC               [GA]^GATC[CT]
              656  GGATCC              
MslI          255  CATCTTCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              948  CACGAGGATG          
NaeI          202  GCCGGC               GCC^GGC
              424  GCCGGC              
NgoMIV        202  GCCGGC               G^CCGGC
              424  GCCGGC              
PflMI         401  CCATGCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              717  CCAGTGCCTGG         
SspBI         944  TGTACA               T^GTACA
              956  TGTACA              
TfiI          303  GATTC                G^A[AT]TC
              472  GATTC               
Van91I        401  CCATGCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              717  CCAGTGCCTGG         
XhoII          89  GGATCC               [GA]^GATC[CT]
              656  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AspHI       AvaI        AvaII       BbvI        BcnI        
BmyI        BpmI        BsaHI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HinfI       HpaII       ItaI        MaeII       MboI        
MboII       MnlI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
TaqI        TseI        TspRI       TthHB8I     
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