Restriction map for pF1KB6414
 (Linear) (Six-base) MAPSORT of: KIBB6414.seq  Check: 1114  from: 1  to: 1005



KIBB6414.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     230     242     686    1005 
   Size:        230      12     444     319

  Fragments arranged by size: 

                 444     319     230      12
 
AfeI AGC'GCT

Cuts at:      0     372    1005 
   Size:        372     633
 
ApaLI G'TGCA_C

Cuts at:      0     137     305    1005 
   Size:        137     168     700

  Fragments arranged by size: 

                 700     168     137
 
ApoI r'AATT_y

Cuts at:      0     276     500     797    1005 
   Size:        276     224     297     208

  Fragments arranged by size: 

                 297     276     224     208
 
BanI G'GyrC_C

Cuts at:      0     117    1005 
   Size:        117     888
 
BanII G_rGCy'C

Cuts at:      0     129     626    1005 
   Size:        129     497     379

  Fragments arranged by size: 

                 497     379     129
 
BbvCI CC'TCA_GC

Cuts at:      0      17     602     908    1005 
   Size:         17     585     306      97

  Fragments arranged by size: 

                 585     306      97      17
 
BfrBI ATG'CAT

Cuts at:      0     346    1005 
   Size:        346     659
 
BglII A'GATC_T

Cuts at:      0     393    1005 
   Size:        393     612
 
BlpI GC'TnA_GC

Cuts at:      0      51    1005 
   Size:         51     954
 
Bme1580I G_kGCm'C

Cuts at:      0     120     141     309    1005 
   Size:        120      21     168     696

  Fragments arranged by size: 

                 696     168     120      21
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      66    1005 
   Size:         66     939
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     638    1005 
   Size:        638     367
 
Bpu10I CC'TnA_GC

Cuts at:      0      17     602     908    1005 
   Size:         17     585     306      97

  Fragments arranged by size: 

                 585     306      97      17
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     446    1005 
   Size:        167     279     559

  Fragments arranged by size: 

                 559     279     167
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      55      82     449    1005 
   Size:         55      27     367     556

  Fragments arranged by size: 

                 556     367      55      27
 
BseYI C'CCAG_C

Cuts at:      0     367    1005 
   Size:        367     638
 
BsiEI CG_ry'CG

Cuts at:      0      35     294    1005 
   Size:         35     259     711

  Fragments arranged by size: 

                 711     259      35
 
BsiHKAI G_wGCw'C

Cuts at:      0     141     309     626    1005 
   Size:        141     168     317     379

  Fragments arranged by size: 

                 379     317     168     141
 
Bsp1286I G_dGCh'C

Cuts at:      0     120     129     141     309     626    1005 
   Size:        120       9      12     168     317     379

  Fragments arranged by size: 

                 379     317     168     120      12       9
 
BstYI r'GATC_y

Cuts at:      0     393    1005 
   Size:        393     612
 
BtgI C'CryG_G

Cuts at:      0      35     121    1005 
   Size:         35      86     884

  Fragments arranged by size: 

                 884      86      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     136     475    1005 
   Size:        136     339     530

  Fragments arranged by size: 

                 530     339     136
 
EaeI y'GGCC_r

Cuts at:      0      32     895    1005 
   Size:         32     863     110

  Fragments arranged by size: 

                 863     110      32
 
EagI C'GGCC_G

Cuts at:      0      32    1005 
   Size:         32     973
 
EarI CTCTTCn'nnn_

Cuts at:      0     548    1005 
   Size:        548     457
 
EcoICRI GAG'CTC

Cuts at:      0     624    1005 
   Size:        624     381
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     230     242     638     686    1005 
   Size:        230      12     396      48     319

  Fragments arranged by size: 

                 396     319     230      48      12
 
EcoRI G'AATT_C

Cuts at:      0     276    1005 
   Size:        276     729
 
EcoRV GAT'ATC

Cuts at:      0     540    1005 
   Size:        540     465
 
HaeII r_GCGC'y

Cuts at:      0     374    1005 
   Size:        374     631
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     529     554     561     586    1005 
   Size:        529      25       7      25     419

  Fragments arranged by size: 

                 529     419      25      25       7
 
HincII GTy'rAC

Cuts at:      0     323    1005 
   Size:        323     682
 
HindIII A'AGCT_T

Cuts at:      0     734     881    1005 
   Size:        734     147     124

  Fragments arranged by size: 

                 734     147     124
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     182    1005 
   Size:        182     823
 
MscI TGG'CCA

Cuts at:      0     897    1005 
   Size:        897     108
 
MspA1I CmG'CkG

Cuts at:      0      25     912    1005 
   Size:         25     887      93

  Fragments arranged by size: 

                 887      93      25
 
NcoI C'CATG_G

Cuts at:      0     121    1005 
   Size:        121     884
 
NsiI A_TGCA'T

Cuts at:      0     348    1005 
   Size:        348     657
 
NspI r_CATG'y

Cuts at:      0     313     346     441    1005 
   Size:        313      33      95     564

  Fragments arranged by size: 

                 564     313      95      33
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     475    1005 
   Size:        475     530
 
PvuII CAG'CTG

Cuts at:      0      25     912    1005 
   Size:         25     887      93

  Fragments arranged by size: 

                 887      93      25
 
SacI G_AGCT'C

Cuts at:      0     626    1005 
   Size:        626     379
 
ScaI AGT'ACT

Cuts at:      0     819    1005 
   Size:        819     186
 
SfcI C'TryA_G

Cuts at:      0      26    1005 
   Size:         26     979
 
SmlI C'TyrA_G

Cuts at:      0     182     461    1005 
   Size:        182     279     544

  Fragments arranged by size: 

                 544     279     182
 
SphI G_CATG'C

Cuts at:      0     441    1005 
   Size:        441     564
 
SspI AAT'ATT

Cuts at:      0     519    1005 
   Size:        519     486
 
StyI C'CwwG_G

Cuts at:      0     121    1005 
   Size:        121     884
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     308    1005 
   Size:        308     697
 
TatI w'GTAC_w

Cuts at:      0     817    1005 
   Size:        817     188
 
XmnI GAAnn'nnTTC

Cuts at:      0     215     503     557     863    1005 
   Size:        215     288      54     306     142

  Fragments arranged by size: 

                 306     288     215     142      54
 

 Enzymes that do cut:

     AcuI     AfeI    ApaLI     ApoI     BanI    BanII    BbvCI    BfrBI
    BglII     BlpI Bme1580I     BplI     BpmI   Bpu10I    BpuEI    BseRI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BstYI     BtgI   DraIII     EaeI
     EagI     EarI  EcoICRI  Eco57MI    EcoRI    EcoRV    HaeII    Hin4I
   HincII  HindIII     MmeI     MscI   MspA1I     NcoI     NsiI     NspI
    PflMI    PvuII     SacI     ScaI     SfcI     SmlI     SphI     SspI
     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI     BcgI
    BciVI     BclI     BglI    BmgBI     BmrI     BmtI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EciI    EcoNI EcoO109I
     FalI     FseI     FspI    FspAI     HpaI     KasI     KpnI     MfeI
     MluI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     StuI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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