Restriction map for pF1KB7694
 (Linear) (Six-base) MAPSORT of: KIBB7694.seq  Check: 7324  from: 1  to: 1239



KIBB7694.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1034    1239 
   Size:       1034     205
 
AleI CACnn'nnGTG

Cuts at:      0    1216    1239 
   Size:       1216      23
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1078    1123    1239 
   Size:       1078      45     116

  Fragments arranged by size: 

                1078     116      45
 
ApaI G_GGCC'C

Cuts at:      0     496    1069    1105    1239 
   Size:        496     573      36     134

  Fragments arranged by size: 

                 573     496     134      36
 
ApaLI G'TGCA_C

Cuts at:      0     771    1239 
   Size:        771     468
 
AvaI C'yCGr_G

Cuts at:      0     147    1239 
   Size:        147    1092
 
BamHI G'GATC_C

Cuts at:      0      57     257     825    1239 
   Size:         57     200     568     414

  Fragments arranged by size: 

                 568     414     200      57
 
BanII G_rGCy'C

Cuts at:      0      41     271     308     427     496     535     955    1069 
   Size:         41     230      37     119      69      39     420     114

Cuts at:   1069    1105    1165    1177    1239 
   Size:         36      60      12      62

  Fragments arranged by size: 

                 420     230     119     114      69      62      60      41
                  39      37      36      12
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     415    1239 
   Size:        415     824
 
BbvCI CC'TCA_GC

Cuts at:      0     197    1239 
   Size:        197    1042
 
BglI GCCn_nnn'nGGC

Cuts at:      0     286    1239 
   Size:        286     953
 
BglII A'GATC_T

Cuts at:      0     366    1239 
   Size:        366     873
 
BlpI GC'TnA_GC

Cuts at:      0     421    1231    1239 
   Size:        421     810       8

  Fragments arranged by size: 

                 810     421       8
 
Bme1580I G_kGCm'C

Cuts at:      0     496     775    1069    1105    1239 
   Size:        496     279     294      36     134

  Fragments arranged by size: 

                 496     294     279     134      36
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      42     211    1072    1168    1239 
   Size:         42     169     861      96      71

  Fragments arranged by size: 

                 861     169      96      71      42
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     418     939    1239 
   Size:        418     521     300

  Fragments arranged by size: 

                 521     418     300
 
Bpu10I CC'TnA_GC

Cuts at:      0     197    1239 
   Size:        197    1042
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     196     226     618     648    1239 
   Size:        196      30     392      30     591

  Fragments arranged by size: 

                 591     392     196      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     338    1239 
   Size:        338     901
 
BseYI C'CCAG_C

Cuts at:      0     284    1012    1239 
   Size:        284     728     227

  Fragments arranged by size: 

                 728     284     227
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     434    1239 
   Size:        434     805
 
BsiHKAI G_wGCw'C

Cuts at:      0     154     775    1239 
   Size:        154     621     464

  Fragments arranged by size: 

                 621     464     154
 
Bsp1286I G_dGCh'C

Cuts at:      0      41     154     271     308     427     496     535     775 
   Size:         41     113     117      37     119      69      39     240

Cuts at:    775     955    1069    1105    1165    1177    1239 
   Size:        180     114      36      60      12      62

  Fragments arranged by size: 

                 240     180     119     117     114     113      69      62
                  60      41      39      37      36      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     456     751    1239 
   Size:        456     295     488

  Fragments arranged by size: 

                 488     456     295
 
BsrBI CCG'CTC

Cuts at:      0     417    1230    1239 
   Size:        417     813       9

  Fragments arranged by size: 

                 813     417       9
 
BsrDI GCAATG_nn'

Cuts at:      0     235     843    1239 
   Size:        235     608     396

  Fragments arranged by size: 

                 608     396     235
 
BsrFI r'CCGG_y

Cuts at:      0     280     875    1239 
   Size:        280     595     364

  Fragments arranged by size: 

                 595     364     280
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     680     779     842    1239 
   Size:        680      99      63     397

  Fragments arranged by size: 

                 680     397      99      63
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     396    1239 
   Size:        396     843
 
BstYI r'GATC_y

Cuts at:      0      57     257     366     825    1239 
   Size:         57     200     109     459     414

  Fragments arranged by size: 

                 459     414     200     109      57
 
Bsu36I CC'TnA_GG

Cuts at:      0     113    1239 
   Size:        113    1126
 
BtgI C'CryG_G

Cuts at:      0     359    1169    1239 
   Size:        359     810      70

  Fragments arranged by size: 

                 810     359      70
 
BtsI GCAGTG_nn'

Cuts at:      0     140     451    1239 
   Size:        140     311     788

  Fragments arranged by size: 

                 788     311     140
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     552     587    1239 
   Size:        552      35     652

  Fragments arranged by size: 

                 652     552      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     870    1239 
   Size:        870     369
 
EaeI y'GGCC_r

Cuts at:      0     877    1239 
   Size:        877     362
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     660     822    1239 
   Size:        660     162     417

  Fragments arranged by size: 

                 660     417     162
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     418     939    1034    1239 
   Size:        418     521      95     205

  Fragments arranged by size: 

                 521     418     205      95
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     186    1239 
   Size:        186    1053
 
EcoO109I rG'GnC_Cy

Cuts at:      0      20      29     445     492     493     716    1066    1102 
   Size:         20       9     416      47       1     223     350      36

Cuts at:   1102    1120    1189    1239 
   Size:         18      69      50

  Fragments arranged by size: 

                 416     350     223      69      50      47      36      20
                  18       9       1
 
HaeII r_GCGC'y

Cuts at:      0     741    1239 
   Size:        741     498
 
HindIII A'AGCT_T

Cuts at:      0     565    1239 
   Size:        565     674
 
MslI CAynn'nnrTG

Cuts at:      0    1216    1239 
   Size:       1216      23
 
MspA1I CmG'CkG

Cuts at:      0     163     169     991    1022    1239 
   Size:        163       6     822      31     217

  Fragments arranged by size: 

                 822     217     163      31       6
 
NaeI GCC'GGC

Cuts at:      0     282    1239 
   Size:        282     957
 
NdeI CA'TA_TG

Cuts at:      0     276    1239 
   Size:        276     963
 
NgoMIV G'CCGG_C

Cuts at:      0     280    1239 
   Size:        280     959
 
PasI CC'CwG_GG

Cuts at:      0      24     262     441    1239 
   Size:         24     238     179     798

  Fragments arranged by size: 

                 798     238     179      24
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      54     360     994    1098    1239 
   Size:         54     306     634     104     141

  Fragments arranged by size: 

                 634     306     141     104      54
 
PfoI T'CCnGG_A

Cuts at:      0     395    1239 
   Size:        395     844
 
PpuMI rG'GwC_Cy

Cuts at:      0      20      29     445    1239 
   Size:         20       9     416     794

  Fragments arranged by size: 

                 794     416      20       9
 
PspOMI G'GGCC_C

Cuts at:      0     492    1065    1101    1239 
   Size:        492     573      36     138

  Fragments arranged by size: 

                 573     492     138      36
 
PstI C_TGCA'G

Cuts at:      0     168    1239 
   Size:        168    1071
 
PvuII CAG'CTG

Cuts at:      0     169     991    1239 
   Size:        169     822     248

  Fragments arranged by size: 

                 822     248     169
 
SanDI GG'GwC_CC

Cuts at:      0      20    1239 
   Size:         20    1219
 
SexAI A'CCwGG_T

Cuts at:      0     866    1239 
   Size:        866     373
 
SfcI C'TryA_G

Cuts at:      0     164     186     204    1239 
   Size:        164      22      18    1035

  Fragments arranged by size: 

                1035     164      22      18
 
StuI AGG'CCT

Cuts at:      0     202    1055    1097    1239 
   Size:        202     853      42     142

  Fragments arranged by size: 

                 853     202     142      42
 
StyI C'CwwG_G

Cuts at:      0    1092    1239 
   Size:       1092     147
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1182    1239 
   Size:       1182      57
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     455     487     817     849    1239 
   Size:        455      32     330      32     390

  Fragments arranged by size: 

                 455     390     330      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     281    1215    1239 
   Size:        281     934      24

  Fragments arranged by size: 

                 934     281      24
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI     ApaI    ApaLI     AvaI    BamHI    BanII
     BbsI    BbvCI     BglI    BglII     BlpI Bme1580I     BmrI     BpmI
   Bpu10I    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI
    BsrBI    BsrDI    BsrFI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
     BtsI    CspCI   DraIII     EaeI     EciI  Eco57MI    EcoNI EcoO109I
    HaeII  HindIII     MslI   MspA1I     NaeI     NdeI   NgoMIV     PasI
    PflMI     PfoI    PpuMI   PspOMI     PstI    PvuII    SanDI    SexAI
     SfcI     StuI     StyI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI     BanI     BbeI     BcgI    BciVI     BclI    BfrBI
    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrGI
   BssHII    BssSI    BstBI   BstEII  BstZ17I    BtgZI     ClaI     DraI
     DrdI     EagI     EarI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NarI     NcoI     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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