Restriction map for
pF1KB6803
(Circular) (Six-base) MAPSORT of: pF1KB6803.seq Check: 2537 from: 1 to: 3590
pF1KB6803 3590 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 236 628 236
Size: 392 3198
Acc65I G'GTAC_C
Cuts at: 606 606
Size: 3590
AclI AA'CG_TT
Cuts at: 3430 3430
Size: 3590
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1415 1847 2594 2862 1415
Size: 432 747 268 2143
Fragments arranged by size:
2143 747 432 268
AfeI AGC'GCT
Cuts at: 182 182
Size: 3590
AflIII A'CryG_T
Cuts at: 942 2046 3023 942
Size: 1104 977 1509
Fragments arranged by size:
1509 1104 977
AgeI A'CCGG_T
Cuts at: 1978 1978
Size: 3590
AlwNI CAG_nnn'CTG
Cuts at: 408 895 2462 408
Size: 487 1567 1536
Fragments arranged by size:
1567 1536 487
ApaLI G'TGCA_C
Cuts at: 2360 2360
Size: 3590
ApoI r'AATT_y
Cuts at: 594 2943 3199 594
Size: 2349 256 985
Fragments arranged by size:
2349 985 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3590
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3590
AvaI C'yCGr_G
Cuts at: 580 610 580
Size: 30 3560
BamHI G'GATC_C
Cuts at: 501 615 501
Size: 114 3476
BanI G'GyrC_C
Cuts at: 169 424 561 606 1020 1268 1303 169
Size: 255 137 45 414 248 35 2456
Fragments arranged by size:
2456 414 255 248 137 45 35
BanII G_rGCy'C
Cuts at: 604 1634 604
Size: 1030 2560
BbeI G_GCGC'C
Cuts at: 428 1024 1272 428
Size: 596 248 2746
Fragments arranged by size:
2746 596 248
BciVI GTATCCnnnnn_n'
Cuts at: 1488 2249 3590 1488
Size: 761 1341 1488
Fragments arranged by size:
1488 1341 761
BclI T'GATC_A
Cuts at: 1110 1110
Size: 3590
BglII A'GATC_T
Cuts at: 1105 3142 1105
Size: 2037 1553
BlpI GC'TnA_GC
Cuts at: 520 702 520
Size: 182 3408
Bme1580I G_kGCm'C
Cuts at: 566 1215 1308 2364 566
Size: 649 93 1056 1792
Fragments arranged by size:
1792 1056 649 93
BmrI ACTGGGnnnn_n'
Cuts at: 567 802 883 1218 3362 567
Size: 235 81 335 2144 795
Fragments arranged by size:
2144 795 335 235 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1957 1957
Size: 3590
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 1645 2137 2435 766
Size: 879 492 298 1921
Fragments arranged by size:
1921 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3294 3294
Size: 3590
BsaAI yAC'GTr
Cuts at: 943 943
Size: 3590
BsaBI GATnn'nnATC
Cuts at: 6 1104 3141 6
Size: 1098 2037 455
Fragments arranged by size:
2037 1098 455
BsaHI Gr'CG_yC
Cuts at: 233 425 491 1021 1269 1971 3467 233
Size: 192 66 530 248 702 1496 356
Fragments arranged by size:
1496 702 530 356 248 192 66
BsaWI w'CCGG_w
Cuts at: 144 779 1300 1978 2252 2399 2810 144
Size: 635 521 678 274 147 411 924
Fragments arranged by size:
924 678 635 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 319 448 550 319
Size: 129 102 3359
Fragments arranged by size:
3359 129 102
BseYI C'CCAG_C
Cuts at: 565 1015 1773 2350 2846 565
Size: 450 758 577 496 1309
Fragments arranged by size:
1309 758 577 496 450
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 442 421
Size: 21 3569
BsiEI CG_ry'CG
Cuts at: 104 1132 1178 2386 104
Size: 1028 46 1208 1308
Fragments arranged by size:
1308 1208 1028 46
BsiHKAI G_wGCw'C
Cuts at: 604 1382 1572 2364 604
Size: 778 190 792 1830
Fragments arranged by size:
1830 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 566 604 1215 1308 1382 1572 1634 2364 566
Size: 38 611 93 74 190 62 730 1792
Fragments arranged by size:
1792 730 611 190 93 74 62 38
BspEI T'CCGG_A
Cuts at: 144 2810 144
Size: 2666 924
BspHI T'CATG_A
Cuts at: 2766 2766
Size: 3590
BspMI ACCTGCnnnn'nnnn_
Cuts at: 464 640 854 1156 1537 464
Size: 176 214 302 381 2517
Fragments arranged by size:
2517 381 302 214 176
BsrBI CCG'CTC
Cuts at: 50 1882 1936 3420 3585 50
Size: 1832 54 1484 165 55
Fragments arranged by size:
1832 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1502 1502
Size: 3590
BsrFI r'CCGG_y
Cuts at: 229 427 1588 1769 1978 229
Size: 198 1161 181 209 1841
Fragments arranged by size:
1841 1161 209 198 181
BssSI C'ACGA_G
Cuts at: 1861 2219 1861
Size: 358 3232
BstAPI GCAn_nnn'nTGC
Cuts at: 192 967 192
Size: 775 2815
BstBI TT'CG_AA
Cuts at: 475 1951 475
Size: 1476 2114
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3590
BstYI r'GATC_y
Cuts at: 1 332 501 615 1082 1105 1440 1686 2698
Size: 331 169 114 467 23 335 246 1012
Cuts at: 2698 2784 2796 3142 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 331 246 169
114 86 23 12
BtgI C'CryG_G
Cuts at: 126 126
Size: 3590
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 306 410 1540 1721 1884 306
Size: 104 1130 181 163 2012
Fragments arranged by size:
2012 1130 181 163 104
BtsI GCAGTG_nn'
Cuts at: 224 459 833 224
Size: 235 374 2981
Fragments arranged by size:
2981 374 235
DraI TTT'AAA
Cuts at: 589 589
Size: 3590
DrdI GACnn_nn'nnGTC
Cuts at: 1296 2154 1296
Size: 858 2732
EaeI y'GGCC_r
Cuts at: 129 1175 1349 1740 1767 3112 129
Size: 1046 174 391 27 1345 607
Fragments arranged by size:
1345 1046 607 391 174 27
EagI C'GGCC_G
Cuts at: 1175 1175
Size: 3590
EarI CTCTTCn'nnn_
Cuts at: 1613 1823 1613
Size: 210 3380
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1798 2108 2254 3400 1798
Size: 310 146 1146 1988
Fragments arranged by size:
1988 1146 310 146
EcoICRI GAG'CTC
Cuts at: 602 602
Size: 3590
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1415 1847 2594 2862 1415
Size: 432 747 268 2143
Fragments arranged by size:
2143 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 580 580
Size: 3590
EcoO109I rG'GnC_Cy
Cuts at: 729 729
Size: 3590
EcoRI G'AATT_C
Cuts at: 594 594
Size: 3590
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1609 1609
Size: 3590
FspI TGC'GCA
Cuts at: 1371 1371
Size: 3590
HaeII r_GCGC'y
Cuts at: 184 428 1024 1272 2294 3273 184
Size: 244 596 248 1022 979 501
Fragments arranged by size:
1022 979 596 501 248 244
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1432 1462 1464 1494 1432
Size: 30 2 30 3528
Fragments arranged by size:
3528 30 30 2
HincII GTy'rAC
Cuts at: 552 629 552
Size: 77 3513
HpaI GTT'AAC
Cuts at: 552 552
Size: 3590
KasI G'GCGC_C
Cuts at: 424 1020 1268 424
Size: 596 248 2746
Fragments arranged by size:
2746 596 248
KpnI G_GTAC'C
Cuts at: 610 610
Size: 3590
MluI A'CGCG_T
Cuts at: 3023 3023
Size: 3590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2261 2445 2261
Size: 184 3406
MscI TGG'CCA
Cuts at: 1351 1351
Size: 3590
MslI CAynn'nnrTG
Cuts at: 1579 1706 1579
Size: 127 3463
MspA1I CmG'CkG
Cuts at: 128 405 701 1015 1375 2388 2633 128
Size: 277 296 314 360 1013 245 1085
Fragments arranged by size:
1085 1013 360 314 296 277 245
NaeI GCC'GGC
Cuts at: 231 429 1771 231
Size: 198 1342 2050
Fragments arranged by size:
2050 1342 198
NarI GG'CG_CC
Cuts at: 425 1021 1269 425
Size: 596 248 2746
Fragments arranged by size:
2746 596 248
NgoMIV G'CCGG_C
Cuts at: 229 427 1769 229
Size: 198 1342 2050
Fragments arranged by size:
2050 1342 198
NspI r_CATG'y
Cuts at: 643 1143 2050 643
Size: 500 907 2183
Fragments arranged by size:
2183 907 500
PciI A'CATG_T
Cuts at: 2046 2046
Size: 3590
PmeI GTTT'AAAC
Cuts at: 589 589
Size: 3590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2729 2761 2729
Size: 32 3558
PstI C_TGCA'G
Cuts at: 332 637 1322 332
Size: 305 685 2600
Fragments arranged by size:
2600 685 305
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3590
PvuII CAG'CTG
Cuts at: 1015 1375 1015
Size: 360 3230
RsrII CG'GwC_CG
Cuts at: 1785 1785
Size: 3590
SacI G_AGCT'C
Cuts at: 604 604
Size: 3590
SacII CC_GC'GG
Cuts at: 129 129
Size: 3590
SalI G'TCGA_C
Cuts at: 627 627
Size: 3590
SapI GCTCTTCn'nnn_
Cuts at: 1613 1823 1613
Size: 210 3380
SbfI CC_TGCA'GG
Cuts at: 637 637
Size: 3590
ScaI AGT'ACT
Cuts at: 3107 3107
Size: 3590
SfcI C'TryA_G
Cuts at: 33 328 633 1318 2311 2502 33
Size: 295 305 685 993 191 1121
Fragments arranged by size:
1121 993 685 305 295 191
SfoI GGC'GCC
Cuts at: 426 1022 1270 426
Size: 596 248 2746
Fragments arranged by size:
2746 596 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3590
SgrAI Cr'CCGG_yG
Cuts at: 427 427
Size: 3590
SmaI CCC'GGG
Cuts at: 612 612
Size: 3590
SmlI C'TyrA_G
Cuts at: 745 1660 2152 2414 745
Size: 915 492 262 1921
Fragments arranged by size:
1921 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3590
SphI G_CATG'C
Cuts at: 643 1143 643
Size: 500 3090
StyI C'CwwG_G
Cuts at: 509 724 1078 509
Size: 215 354 3021
Fragments arranged by size:
3021 354 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 549 1173 1306 1765 1974 549
Size: 624 133 459 209 2165
Fragments arranged by size:
2165 624 459 209 133
TatI w'GTAC_w
Cuts at: 3105 3105
Size: 3590
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 153 185 3107 3139 153
Size: 32 2922 32 604
Fragments arranged by size:
2922 604 32 32
Tth111I GACn'n_nGTC
Cuts at: 1387 1387
Size: 3590
XbaI T'CTAG_A
Cuts at: 621 621
Size: 3590
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1085 1085
Size: 3590
XmaI C'CCGG_G
Cuts at: 610 610
Size: 3590
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI
SfiI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI